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Add authors to all workflows #291

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merged 6 commits into from
Nov 23, 2023

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lldelisle
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I ran for f in $(find $(pwd) -name ".dockstore.yml"); do echo $f; cd $(dirname $f); planemo dockstore_init .; done and then manually check if it was a real change or just a reordering of workflows and manually update version and CHANGELOG.md.

  • I still have the sars-cov-2 to do where all *test.yml should be *tests.yml to be discovered by dockstore.
  • It seems that all VGP also needs to be updated.

@lldelisle lldelisle marked this pull request as draft November 20, 2023 15:04
@lldelisle
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@mvdbeek do you have a script to do a minor release with an automatic message to put in CHANGELOG.md?

## [0.1.7] 2023-11-20

- Fix author in dockstore

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There is no changelog for 0.1.6 @mvdbeek @Delphine-L

@lldelisle lldelisle marked this pull request as ready for review November 20, 2023 21:06
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@mvdbeek do you have a script to do a minor release with an automatic message to put in CHANGELOG.md?

I did what I tell my biologist not to do... I did it manually like a monkey...

Anyhow, it is done.

@lldelisle
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Maybe I need to split this PR into pieces because the deployment will probably be skipped because lint will fail as well as other tests, no?

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mvdbeek commented Nov 21, 2023

There is https://github.com/galaxyproject/iwc/blob/main/bump_version.py for bumping versions. This slipped past review though, we don't support the unreleased section in planemo and it would appear in the changelogs if not removed.

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mvdbeek commented Nov 21, 2023

Maybe I need to split this PR into pieces because the deployment will probably be skipped because lint will fail as well as other tests, no?

Right ... maybe we should skip tests on merge ?

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Here is the report of tests:

Test Results (powered by Planemo)

Test Summary

Test State Count
Total 22
Passed 16
Error 6
Failure 0
Skipped 0
Errored Tests
  • ❌ gromacs-mmgbsa.ga_0

    Execution Problem:

    • Unexpected HTTP status code: 400: {"err_msg": "Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2021.3+galaxy1 (version 2021.3+galaxy1)", "err_code": 0, "traceback": "Traceback (most recent call last):\n  File \"/tmp/tmpr1qf14tn/galaxy-dev/lib/galaxy/web/framework/decorators.py\", line 337, in decorator\n    rval = func(self, trans, *args, **kwargs)\n  File \"/tmp/tmpr1qf14tn/galaxy-dev/lib/galaxy/webapps/galaxy/api/workflows.py\", line 759, in invoke\n    raise exceptions.MessageException(missing_tools_message)\ngalaxy.exceptions.MessageException: Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/chemteam/gmx_solvate/gmx_solvate/2021.3+galaxy1 (version 2021.3+galaxy1)\n"}
      
  • ❌ ont-artic-variation.ga_0

    Execution Problem:

    • Failed to upload data, upload state is [error].
      
  • ❌ pe-artic-variation.ga_0

    Execution Problem:

    • Failed to upload data, upload state is [error].
      
  • ❌ pe-wgs-ivar-analysis.ga_0

    Execution Problem:

    • Unexpected HTTP status code: 400: {"err_msg": "Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/3.1.14+galaxy0 (version 3.1.14+galaxy0)", "err_code": 0, "traceback": "Traceback (most recent call last):\n  File \"/tmp/tmp4kta3c32/galaxy-dev/lib/galaxy/web/framework/decorators.py\", line 337, in decorator\n    rval = func(self, trans, *args, **kwargs)\n  File \"/tmp/tmp4kta3c32/galaxy-dev/lib/galaxy/webapps/galaxy/api/workflows.py\", line 759, in invoke\n    raise exceptions.MessageException(missing_tools_message)\ngalaxy.exceptions.MessageException: Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/3.1.14+galaxy0 (version 3.1.14+galaxy0)\n"}
      
  • ❌ pe-wgs-variation.ga_0

    Execution Problem:

    • Failed to upload data, upload state is [error].
      
  • ❌ se-wgs-variation.ga_0

    Execution Problem:

    • Failed to upload data, upload state is [error].
      

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mvdbeek commented Nov 21, 2023

The error in the gromacs workflow is real, for the rest it's SRA data that isn't available anymore.

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Indeed I was making the list:

@lldelisle
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For pe-wgs-ivar-analysis, the version 3.1.14+galaxy0 with changeset_revision fe3e8506112c has disappeared from the toolshed (probably number 10) as another file with the same version has been uploaded but this version is not a valid tool: https://toolshed.g2.bx.psu.edu/repository?repository_id=aaec941c4dfce850&changeset_revision=073de3fb84a0
I guess this is also the reason why there is no update anymore.

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mvdbeek commented Nov 21, 2023

yep, if you go to https://toolshed.g2.bx.psu.edu/repository?repository_id=aaec941c4dfce850&changeset_revision=fe3e8506112c you can see this is a broken revision

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mvdbeek commented Nov 21, 2023

(though I think you should still be able to trigger an installation for a changeset revision that is not installable, in which case you should get the next installable revision)

@lldelisle
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But it is installed on org:
image

@lldelisle
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Should I put all sars-cov2 fastqs on zenodo?

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mvdbeek commented Nov 21, 2023

I was working on a script to do that, but maybe we should just do that manually until they've actually clarified which API should be used for that.

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(I put the whole fastq from the SRA, right?)

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mvdbeek commented Nov 21, 2023

yes

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I am closer:

Test Results (powered by Planemo)

Test Summary

Test State Count
Total 22
Passed 19
Error 2
Failure 1
Skipped 0
Errored Tests
  • ❌ pe-wgs-ivar-analysis.ga_0

    Execution Problem:

    • Unexpected HTTP status code: 400: {"err_msg": "Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/3.1.14+galaxy0 (version 3.1.14+galaxy0)", "err_code": 0, "traceback": "Traceback (most recent call last):\n  File \"/tmp/tmpvq20aobg/galaxy-dev/lib/galaxy/web/framework/decorators.py\", line 337, in decorator\n    rval = func(self, trans, *args, **kwargs)\n  File \"/tmp/tmpvq20aobg/galaxy-dev/lib/galaxy/webapps/galaxy/api/workflows.py\", line 759, in invoke\n    raise exceptions.MessageException(missing_tools_message)\ngalaxy.exceptions.MessageException: Workflow was not invoked; the following required tools are not installed: toolshed.g2.bx.psu.edu/repos/iuc/pangolin/pangolin/3.1.14+galaxy0 (version 3.1.14+galaxy0)\n"}
      
  • ❌ se-wgs-variation.ga_0

    Execution Problem:

    • Failed to upload data, upload state is [error].
      
Failed Tests
  • ❌ pe-artic-variation.ga_0

    Problems:

    • Output with path /tmp/tmp66myyl1m/Final (SnpEff-) annotated variants with strand-bias soft filter applied__4fff629b-af3f-45b2-b5f8-0c8669cd20bc different than expected, difference (using diff):
      ( /home/runner/work/iwc/iwc/workflows/sars-cov-2-variant-calling/sars-cov-2-pe-illumina-artic-variant-calling/test-data/final_snpeff_annotated_variants.vcf v. /tmp/tmpbis5pie0final_snpeff_annotated_variants.vcf )
      --- local_file
      +++ history_data
      @@ -1,7 +1,7 @@
       ##fileformat=VCFv4.0
       ##FILTER=<ID=PASS,Description="All filters passed">
      -##fileDate=20220208
      -##source=lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter --no-default-filter -r NC_045512.2:1-7475 -o /data/jwd/main/040/985/40985665/tmp/lofreq2_call_parallelfwy160pl/0.vcf.gz reads.bam
      +##fileDate=20231121
      +##source=lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter --no-default-filter -r NC_045512.2:1-14951 -o /tmp/tmpjh25ezu9/tmp/lofreq2_call_paralleljz94xt5b/0.vcf.gz reads.bam 
       ##reference=reference.fa
       ##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw Depth">
       ##INFO=<ID=AF,Number=1,Type=Float,Description="Lofreq Allele Frequency; Fraction of variant-supporting bases with q > --min-bq among all bases at the site">
      @@ -17,10 +17,10 @@
       ##contig=<ID=NC_045512.2>
       ##INFO=<ID=AmpliconBias,Number=0,Type=Flag,Description="Indicates that the AF value of the variant could not be corrected for potential amplicon bias.">
       ##bcftools_annotateVersion=1.10.2+htslib-1.10.2
      -##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites -AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Feb  8 11:30:28 2022
      -##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites +AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Feb  8 11:33:07 2022
      +##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites -AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Nov 21 22:22:42 2023
      +##bcftools_annotateCommand=annotate --columns QUAL,INFO --annotations annotations.vcf.gz --mark-sites +AmpliconBias --output-type v --threads 1 input.vcf.gz; Date=Tue Nov 21 22:22:53 2023
       ##SnpEffVersion="4.5covid19 (build 2020-04-15 22:26), by Pablo Cingolani"
      -##SnpEffCmd="SnpEff  -i vcf -o vcf -formatEff -classic -no-downstream -no-intergenic -no-upstream -stats /data/jwd/main/040/985/40985835/outputs/galaxy_dataset_6f12bbaa-aa2a-4071-ab5d-da54e3a4f14a.dat NC_045512.2 /data/dnb05/galaxy_db/files/6/2/f/dataset_62f5f01c-2e71-49f6-9ec8-206545e560b1.dat "
      +##SnpEffCmd="SnpEff  -i vcf -o vcf -formatEff -classic -no-downstream -no-intergenic -no-upstream -stats /tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293.dat NC_045512.2 /tmp/tmpjh25ezu9/files/2/b/6/dataset_2b6b0315-3f83-4e60-9650-eea0be108e37.dat "
       ##INFO=<ID=EFF,Number=.,Type=String,Description="Predicted effects for this variant.Format: 'Effect ( Effect_Impact | Functional_Class | Codon_Change | Amino_Acid_Change| Amino_Acid_length | Gene_Name | Transcript_BioType | Gene_Coding | Transcript_ID | Exon_Rank  | Genotype [ | ERRORS | WARNINGS ] )' ">
       ##INFO=<ID=LOF,Number=.,Type=String,Description="Predicted loss of function effects for this variant. Format: 'Gene_Name | Gene_ID | Number_of_transcripts_in_gene | Percent_of_transcripts_affected'">
       ##INFO=<ID=NMD,Number=.,Type=String,Description="Predicted nonsense mediated decay effects for this variant. Format: 'Gene_Name | Gene_ID | Number_of_transcripts_in_gene | Percent_of_transcripts_affected'">
      @@ -32,7 +32,7 @@
       NC_045512.2	490	.	T	A	218	PASS	DP=108;AF=0.092593;SB=0;DP4=63,35,6,4;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaT/gaA|D75E|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaT/gaA|D75E|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|A|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaT/gaA|D75E|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|A|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaT/gaA|D75E|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A)
       NC_045512.2	732	.	A	T	86	sb_fdr	DP=16752;AF=0.001552;SB=441;DP4=14805,1896,0,47;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D156V|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D156V|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D156V|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D156V|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T)
       NC_045512.2	754	.	TA	T	89	PASS	DP=19291;AF=0.00057;SB=1;DP4=16545,2735,9,2;INDEL;HRUN=3;EFF=FRAME_SHIFT(HIGH||aaa/|K164|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|T|INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|T|WARNING_TRANSCRIPT_NO_STOP_CODON&INFO_REALIGN_3_PRIME),FRAME_SHIFT(HIGH||aaa/|K164|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|T|INFO_REALIGN_3_PRIME);LOF=(ORF1ab|GU280_gp01|28|0.07)
      -NC_045512.2	802	.	A	G	74	PASS	DP=29038;AF=0.001825;SB=18;DP4=21564,6726,55,30;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G)
      +NC_045512.2	802	.	A	G	74	PASS	DP=29046;AF=0.001825;SB=18;DP4=21572,6726,55,30;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G)
       NC_045512.2	829	.	C	A	1172	sb_fdr	DP=29782;AF=0.007253;SB=492;DP4=16581,12865,289,18;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N188K|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N188K|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|A),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N8K|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aaC/aaA|N8K|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|A|WARNING_TRANSCRIPT_NO_START_CODON)
       NC_045512.2	840	.	G	C	156	sb_fdr	DP=28966;AF=0.002037;SB=204;DP4=15043,13776,9,96;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G192A|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G192A|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G12A|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gCc|G12A|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON)
       NC_045512.2	844	.	T	C	146	sb_fdr	DP=29542;AF=0.001625;SB=155;DP4=14184,15238,86,12;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P193|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P193|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|C),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P13|637|ORF1ab|protein_coding|CODING|YP_009725298.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON),SYNONYMOUS_CODING(LOW|SILENT|ccT/ccC|P13|637|ORF1ab|protein_coding|CODING|YP_009742609.1|1|C|WARNING_TRANSCRIPT_NO_START_CODON)
      
      

    Workflow invocation details

    • Invocation Messages

    • Steps
      • Step 1: Paired Collection:

        • step_state: scheduled
      • Step 2: NC_045512.2 FASTA sequence of SARS-CoV-2:

        • step_state: scheduled
      • Step 11: toolshed.g2.bx.psu.edu/repos/iuc/compose_text_param/compose_text_param/0.1.1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • cd ../; python _evaluate_expression_.py

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              components [{"__index__": 0, "param_type": {"__current_case__": 0, "component_value": "( DP > ", "select_param_type": "text"}}, {"__index__": 1, "param_type": {"__current_case__": 1, "component_value": "1", "select_param_type": "integer"}}, {"__index__": 2, "param_type": {"__current_case__": 0, "component_value": " ) & ( ( AF * DP ) >= ( ", "select_param_type": "text"}}, {"__index__": 3, "param_type": {"__current_case__": 1, "component_value": "10", "select_param_type": "integer"}}, {"__index__": 4, "param_type": {"__current_case__": 0, "component_value": " - 0.5 ) )", "select_param_type": "text"}}]
              dbkey "?"
      • Step 12: toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa_mem/0.7.17.2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • set -o | grep -q pipefail && set -o pipefail;  ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' 'localref.fa' && bwa index 'localref.fa' &&    bwa mem  -t "${GALAXY_SLOTS:-1}" -v 1                 'localref.fa' '/tmp/tmpjh25ezu9/files/6/6/7/dataset_6677d982-521c-43d0-bea8-238c3c90ce3a.dat' '/tmp/tmpjh25ezu9/files/b/d/9/dataset_bd98e280-1ab3-4214-b195-3d88db763ac0.dat'  | samtools sort -@${GALAXY_SLOTS:-2} -T "${TMPDIR:-.}" -O bam -o '/tmp/tmpjh25ezu9/job_working_directory/000/93/outputs/dataset_bf2517d4-987b-464d-8868-150de1124074.dat'

            Exit Code:

            • 0

            Standard Error:

            • [bwa_index] Pack FASTA... 0.00 sec
              [bwa_index] Construct BWT for the packed sequence...
              [bwa_index] 0.00 seconds elapse.
              [bwa_index] Update BWT... 0.00 sec
              [bwa_index] Pack forward-only FASTA... 0.00 sec
              [bwa_index] Construct SA from BWT and Occ... 0.00 sec
              [main] Version: 0.7.17-r1188
              [main] CMD: bwa index localref.fa
              [main] Real time: 0.011 sec; CPU: 0.009 sec
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (188, 240, 293)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
              [M::mem_pestat] mean and std.dev: (236.09, 72.41)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (188, 239, 293)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
              [M::mem_pestat] mean and std.dev: (235.71, 72.44)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (186, 239, 293)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
              [M::mem_pestat] mean and std.dev: (235.25, 72.64)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (187, 240, 293)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
              [M::mem_pestat] mean and std.dev: (235.77, 72.25)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (186, 238, 292)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
              [M::mem_pestat] mean and std.dev: (234.79, 72.73)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [M::mem_pestat] skip orientation FF as there are not enough pairs
              [M::mem_pestat] analyzing insert size distribution for orientation FR...
              [M::mem_pestat] (25, 50, 75) percentile: (185, 238, 292)
              [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
              [M::mem_pestat] mean and std.dev: (234.12, 72.68)
              [M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
              [M::mem_pestat] skip orientation RF as there are not enough pairs
              [M::mem_pestat] skip orientation RR as there are not enough pairs
              [main] Version: 0.7.17-r1188
              [main] CMD: bwa mem -t 1 -v 1 localref.fa /tmp/tmpjh25ezu9/files/6/6/7/dataset_6677d982-521c-43d0-bea8-238c3c90ce3a.dat /tmp/tmpjh25ezu9/files/b/d/9/dataset_bd98e280-1ab3-4214-b195-3d88db763ac0.dat
              [main] Real time: 11.835 sec; CPU: 10.722 sec
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "fasta"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              analysis_type {"__current_case__": 0, "analysis_type_selector": "illumina"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              fastq_input {"__current_case__": 2, "fastq_input1": {"values": [{"id": 149, "src": "dce"}]}, "fastq_input_selector": "paired_collection", "iset_stats": ""}
              output_sort "coordinate"
              reference_source {"__current_case__": 1, "index_a": "auto", "ref_file": {"values": [{"id": 173, "src": "hda"}]}, "reference_source_selector": "history"}
              rg {"__current_case__": 3, "rg_selector": "do_not_set"}
      • Step 13: toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.9+galaxy2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • addthreads=${GALAXY_SLOTS:-1} && (( addthreads-- )) &&     ln -s '/tmp/tmpjh25ezu9/files/b/f/2/dataset_bf2517d4-987b-464d-8868-150de1124074.dat' infile && ln -s '/tmp/tmpjh25ezu9/files/_metadata_files/b/f/a/metadata_bfab25e4-b38c-4529-9947-f4093b513876.dat' infile.bai &&         samtools view -@ $addthreads -b  -q 20 -f 1 -F 268    -o outfile      infile

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "bam"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              addref_cond {"__current_case__": 0, "addref_select": "no"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              mode {"__current_case__": 1, "filter_config": {"cigarcons": null, "cond_region": {"__current_case__": 0, "select_region": "no"}, "cond_rg": {"__current_case__": 0, "select_rg": "no"}, "exclusive_filter": ["4", "8", "256"], "exclusive_filter_all": null, "inclusive_filter": ["1"], "library": "", "quality": "20"}, "output_options": {"__current_case__": 0, "adv_output": {"collapsecigar": false, "readtags": []}, "complementary_output": false, "output_format": {"__current_case__": 2, "oformat": "bam"}, "reads_report_type": "retained"}, "outtype": "selected_reads", "subsample_config": {"subsampling_mode": {"__current_case__": 0, "factor": "1.0", "seed": null, "select_subsample": "fraction"}}}
      • Step 14: toolshed.g2.bx.psu.edu/repos/iuc/lofreq_viterbi/lofreq_viterbi/2.1.5+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' reference.fa && lofreq faidx reference.fa 2>&1 || echo "Error running samtools faidx for indexing fasta reference for lofreq" >&2 &&  lofreq viterbi --ref 'reference.fa'  --defqual 2 --out tmp.bam '/tmp/tmpjh25ezu9/files/9/e/f/dataset_9ef8fc80-9960-49da-b05e-54eab0e06cbb.dat' &&  samtools sort --no-PG -T "${TMPDIR:-.}" -@ ${GALAXY_SLOTS:-1} -O BAM -o '/tmp/tmpjh25ezu9/job_working_directory/000/95/outputs/dataset_32a3da80-ca12-447c-b2cb-280399890e0e.dat' tmp.bam

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              adv_options {"bq2_handling": {"__current_case__": 0, "defqual": "2", "replace_bq2": "keep"}, "keepflags": false}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              reference_source {"__current_case__": 1, "ref": {"values": [{"id": 173, "src": "hda"}]}, "ref_selector": "history"}
      • Step 15: toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.2+galaxy2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • addthreads=${GALAXY_SLOTS:-1} && (( addthreads-- )) &&   ln -s '/tmp/tmpjh25ezu9/files/9/e/f/dataset_9ef8fc80-9960-49da-b05e-54eab0e06cbb.dat' infile && ln -s '/tmp/tmpjh25ezu9/files/_metadata_files/0/9/8/metadata_09800823-5192-4396-9ce1-49fad9bcfd95.dat' infile.bai &&    samtools stats       -@ $addthreads infile   > '/tmp/tmpjh25ezu9/job_working_directory/000/96/outputs/dataset_f15aaf73-bc37-4ef7-b293-14cadb50dff2.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "bam"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              addref_cond {"__current_case__": 0, "addref_select": "no"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              cond_region {"__current_case__": 0, "select_region": "no"}
              cov_threshold None
              coverage_cond {"__current_case__": 0, "coverage_select": "no"}
              dbkey "?"
              filter_by_flags {"__current_case__": 1, "filter_flags": "nofilter"}
              gc_depth None
              insert_size None
              most_inserts None
              read_length None
              remove_dups false
              remove_overlaps false
              sparse false
              split_output_cond {"__current_case__": 0, "split_output_selector": "no"}
              trim_quality None
      • Step 16: toolshed.g2.bx.psu.edu/repos/iuc/lofreq_indelqual/lofreq_indelqual/2.1.5+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' reference.fa && lofreq faidx reference.fa 2>&1 || echo "Error running samtools faidx for indexing fasta reference for lofreq" >&2 &&  lofreq indelqual --dindel --ref reference.fa -o output.bam /tmp/tmpjh25ezu9/files/3/2/a/dataset_32a3da80-ca12-447c-b2cb-280399890e0e.dat

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              strategy {"__current_case__": 1, "reference_source": {"__current_case__": 1, "ref": {"values": [{"id": 173, "src": "hda"}]}, "ref_selector": "history"}, "selector": "dindel"}
      • Step 17: toolshed.g2.bx.psu.edu/repos/iuc/ivar_trim/ivar_trim/1.3.1+galaxy2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • cp '/tmp/tmpjh25ezu9/files/0/f/e/dataset_0fe859b3-49b9-45e8-92ca-f5ed526f76a8.dat' bed.bed && python '/tmp/shed_dir/toolshed.g2.bx.psu.edu/repos/iuc/ivar_trim/c092052ed673/ivar_trim/sanitize_bed.py' bed.bed && ln -s '/tmp/tmpjh25ezu9/files/2/d/b/dataset_2dbd7a96-50ca-4a34-9548-be57064cdc4f.dat' amplicon_info_raw.tsv && python '/tmp/shed_dir/toolshed.g2.bx.psu.edu/repos/iuc/ivar_trim/c092052ed673/ivar_trim/prepare_amplicon_info.py' bed.bed amplicon_info_raw.tsv amplicon_info.tsv && ln -s '/tmp/tmpjh25ezu9/files/2/0/6/dataset_206ce7fa-32c8-414d-afa9-8ce0d3b60463.dat' sorted.bam && ln -s '/tmp/tmpjh25ezu9/files/_metadata_files/5/5/7/metadata_557c51b6-3f98-4d5d-8e21-34eaf7df5227.dat' sorted.bam.bai &&  ivar trim -i sorted.bam -b bed.bed -f amplicon_info.tsv -x 0 -e -m 1 -q 0 -s 4 -p trimmed && samtools sort -@ ${GALAXY_SLOTS:-1} -o trimmed.sorted.bam trimmed.bam

            Exit Code:

            • 0

            Standard Output:

            • Found 218 primers in BED file
              Amplicons detected: 
              [30, 410] max = 29866
              [320, 726] max = 29866
              [642, 1028] max = 29866
              [943, 1337] max = 29866
              [1242, 1651] max = 29866
              [1573, 1964] max = 29866
              [1868, 2269] max = 29866
              [2181, 2592] max = 29866
              [2504, 2904] max = 29866
              [2826, 3210] max = 29866
              [3144, 3531] max = 29866
              [3460, 3853] max = 29866
              [3771, 4164] max = 29866
              [4044, 4450] max = 29866
              [4294, 4696] max = 29866
              [4636, 5017] max = 29866
              [4939, 5321] max = 29866
              [5230, 5644] max = 29866
              [5563, 5957] max = 29866
              [5867, 6272] max = 29866
              [6167, 6550] max = 29866
              [6466, 6873] max = 29866
              [6718, 7117] max = 29866
              [7035, 7415] max = 29866
              [7305, 7694] max = 29866
              [7626, 8019] max = 29866
              [7943, 8341] max = 29866
              [8249, 8661] max = 29866
              [8595, 8983] max = 29866
              [8888, 9271] max = 29866
              [9204, 9585] max = 29866
              [9477, 9858] max = 29866
              [9784, 10171] max = 29866
              [10076, 10459] max = 29866
              [10362, 10763] max = 29866
              [10666, 11074] max = 29866
              [10999, 11394] max = 29866
              [11306, 11693] max = 29866
              [11555, 11949] max = 29866
              [11863, 12256] max = 29866
              [12110, 12490] max = 29866
              [12417, 12802] max = 29866
              [12710, 13096] max = 29866
              [13005, 13400] max = 29866
              [13307, 13699] max = 29866
              [13599, 13984] max = 29866
              [13918, 14299] max = 29866
              [14207, 14601] max = 29866
              [14545, 14926] max = 29866
              [14865, 15246] max = 29866
              [15171, 15560] max = 29866
              [15481, 15886] max = 29866
              [15827, 16209] max = 29866
              [16118, 16510] max = 29866
              [16416, 16833] max = 29866
              [16748, 17152] max = 29866
              [17065, 17452] max = 29866
              [17381, 17761] max = 29866
              [17674, 18062] max = 29866
              [17966, 18348] max = 29866
              [18253, 18672] max = 29866
              [18596, 18979] max = 29866
              [18896, 19297] max = 29866
              [19204, 19616] max = 29866
              [19548, 19939] max = 29866
              [19844, 20255] max = 29866
              [20172, 20572] max = 29866
              [20472, 20890] max = 29866
              [20786, 21169] max = 29866
              [21075, 21455] max = 29866
              [21357, 21743] max = 29866
              [21658, 22038] max = 29866
              [21961, 22346] max = 29866
              [22262, 22650] max = 29866
              [22516, 22903] max = 29866
              [22797, 23214] max = 29866
              [23122, 23522] max = 29866
              [23443, 23847] max = 29866
              [23789, 24169] max = 29866
              [24078, 24467] max = 29866
              [24391, 24789] max = 29866
              [24696, 25076] max = 29866
              [24978, 25369] max = 29866
              [25279, 25673] max = 29866
              [25601, 25994] max = 29866
              [25902, 26315] max = 29866
              [26197, 26590] max = 29866
              [26520, 26913] max = 29866
              [26835, 27227] max = 29866
              [27141, 27533] max = 29866
              [27446, 27854] max = 29866
              [27784, 28172] max = 29866
              [28081, 28464] max = 29866
              [28394, 28779] max = 29866
              [28677, 29063] max = 29866
              [28985, 29378] max = 29866
              [29288, 29693] max = 29866
              [29486, 29866] max = 29866
              
              Number of references in file: 1
              NC_045512.2
              Using Region: NC_045512.2
              
              Found 391231 mapped reads
              Found 0 unmapped reads
              Sorted By Coordinate
              -------
              Processed 10% reads ... 
              Processed 20% reads ... 
              Processed 30% reads ... 
              Processed 40% reads ... 
              Processed 50% reads ... 
              Processed 60% reads ... 
              Processed 70% reads ... 
              Processed 80% reads ... 
              Processed 90% reads ... 
              
              -------
              Results: 
              Primer Name	Read Count
              nCoV-2019_1_LEFT	236
              nCoV-2019_1_RIGHT	128
              nCoV-2019_2_LEFT	20
              nCoV-2019_2_RIGHT	48
              nCoV-2019_3_LEFT	3882
              nCoV-2019_3_RIGHT	5189
              nCoV-2019_4_LEFT	8981
              nCoV-2019_4_RIGHT	10479
              nCoV-2019_5_LEFT	66
              nCoV-2019_5_RIGHT	3
              nCoV-2019_6_LEFT	0
              nCoV-2019_6_RIGHT	2
              nCoV-2019_7_LEFT	6
              nCoV-2019_7_LEFT_alt0	0
              nCoV-2019_7_RIGHT	0
              nCoV-2019_7_RIGHT_alt5	7
              nCoV-2019_8_LEFT	7
              nCoV-2019_8_RIGHT	20
              nCoV-2019_9_LEFT	23
              nCoV-2019_9_LEFT_alt4	0
              nCoV-2019_9_RIGHT	0
              nCoV-2019_9_RIGHT_alt2	9
              nCoV-2019_10_LEFT	55
              nCoV-2019_10_RIGHT	30
              nCoV-2019_11_LEFT	16
              nCoV-2019_11_RIGHT	11
              nCoV-2019_12_LEFT	7
              nCoV-2019_12_RIGHT	2
              nCoV-2019_13_LEFT	8
              nCoV-2019_13_RIGHT	10
              nCoV-2019_14_LEFT	7
              nCoV-2019_14_LEFT_alt4	1
              nCoV-2019_14_RIGHT	3
              nCoV-2019_14_RIGHT_alt2	17
              nCoV-2019_15_LEFT	0
              nCoV-2019_15_LEFT_alt1	12
              nCoV-2019_15_RIGHT	0
              nCoV-2019_15_RIGHT_alt3	12
              nCoV-2019_16_LEFT	80
              nCoV-2019_16_RIGHT	29
              nCoV-2019_17_LEFT	19
              nCoV-2019_17_RIGHT	18
              nCoV-2019_18_LEFT	0
              nCoV-2019_18_LEFT_alt2	30
              nCoV-2019_18_RIGHT	32
              nCoV-2019_18_RIGHT_alt1	0
              nCoV-2019_19_LEFT	5
              nCoV-2019_19_RIGHT	5
              nCoV-2019_20_LEFT	54
              nCoV-2019_20_RIGHT	28
              nCoV-2019_21_LEFT	0
              nCoV-2019_21_LEFT_alt2	9
              nCoV-2019_21_RIGHT	0
              nCoV-2019_21_RIGHT_alt0	2
              nCoV-2019_22_LEFT	77
              nCoV-2019_22_RIGHT	54
              nCoV-2019_23_LEFT	9
              nCoV-2019_23_RIGHT	8
              nCoV-2019_24_LEFT	62
              nCoV-2019_24_RIGHT	17
              nCoV-2019_25_LEFT	7
              nCoV-2019_25_RIGHT	3
              nCoV-2019_26_LEFT	33
              nCoV-2019_26_RIGHT	25
              nCoV-2019_27_LEFT	107
              nCoV-2019_27_RIGHT	35
              nCoV-2019_28_LEFT	13
              nCoV-2019_28_RIGHT	38
              nCoV-2019_29_LEFT	0
              nCoV-2019_29_RIGHT	8
              nCoV-2019_30_LEFT	11
              nCoV-2019_30_RIGHT	22
              nCoV-2019_31_LEFT	34
              nCoV-2019_31_RIGHT	22
              nCoV-2019_32_LEFT	26
              nCoV-2019_32_RIGHT	10
              nCoV-2019_33_LEFT	10
              nCoV-2019_33_RIGHT	19
              nCoV-2019_34_LEFT	48
              nCoV-2019_34_RIGHT	53
              nCoV-2019_35_LEFT	14
              nCoV-2019_35_RIGHT	27
              nCoV-2019_36_LEFT	17
              nCoV-2019_36_RIGHT	27
              nCoV-2019_37_LEFT	35
              nCoV-2019_37_RIGHT	51
              nCoV-2019_38_LEFT	20
              nCoV-2019_38_RIGHT	22
              nCoV-2019_39_LEFT	97
              nCoV-2019_39_RIGHT	26
              nCoV-2019_40_LEFT	23
              nCoV-2019_40_RIGHT	17
              nCoV-2019_41_LEFT	49
              nCoV-2019_41_RIGHT	21
              nCoV-2019_42_LEFT	1023
              nCoV-2019_42_RIGHT	2409
              nCoV-2019_43_LEFT	26
              nCoV-2019_43_RIGHT	8
              nCoV-2019_44_LEFT	0
              nCoV-2019_44_LEFT_alt3	58
              nCoV-2019_44_RIGHT	0
              nCoV-2019_44_RIGHT_alt0	89
              nCoV-2019_45_LEFT	3
              nCoV-2019_45_LEFT_alt2	4
              nCoV-2019_45_RIGHT	0
              nCoV-2019_45_RIGHT_alt7	4
              nCoV-2019_46_LEFT	0
              nCoV-2019_46_LEFT_alt1	4
              nCoV-2019_46_RIGHT	0
              nCoV-2019_46_RIGHT_alt2	20
              nCoV-2019_47_LEFT	33
              nCoV-2019_47_RIGHT	22
              nCoV-2019_48_LEFT	35
              nCoV-2019_48_RIGHT	27
              nCoV-2019_49_LEFT	48
              nCoV-2019_49_RIGHT	44
              nCoV-2019_50_LEFT	34
              nCoV-2019_50_RIGHT	21
              nCoV-2019_51_LEFT	25
              nCoV-2019_51_RIGHT	17
              nCoV-2019_52_LEFT	52
              nCoV-2019_52_RIGHT	63
              nCoV-2019_53_LEFT	26
              nCoV-2019_53_RIGHT	23
              nCoV-2019_54_LEFT	51
              nCoV-2019_54_RIGHT	23
              nCoV-2019_55_LEFT	77
              nCoV-2019_55_RIGHT	84
              nCoV-2019_56_LEFT	5
              nCoV-2019_56_RIGHT	18
              nCoV-2019_57_LEFT	84
              nCoV-2019_57_RIGHT	72
              nCoV-2019_58_LEFT	29
              nCoV-2019_58_RIGHT	29
              nCoV-2019_59_LEFT	8
              nCoV-2019_59_RIGHT	2
              nCoV-2019_60_LEFT	30
              nCoV-2019_60_RIGHT	38
              nCoV-2019_61_LEFT	34
              nCoV-2019_61_RIGHT	42
              nCoV-2019_62_LEFT	18
              nCoV-2019_62_RIGHT	14
              nCoV-2019_63_LEFT	35
              nCoV-2019_63_RIGHT	36
              nCoV-2019_64_LEFT	1
              nCoV-2019_64_RIGHT	1
              nCoV-2019_65_LEFT	31
              nCoV-2019_65_RIGHT	61
              nCoV-2019_66_LEFT	6
              nCoV-2019_66_RIGHT	3
              nCoV-2019_67_LEFT	910
              nCoV-2019_67_RIGHT	503
              nCoV-2019_68_LEFT	58
              nCoV-2019_68_RIGHT	144
              nCoV-2019_69_LEFT	7504
              nCoV-2019_69_RIGHT	5814
              nCoV-2019_70_LEFT	43
              nCoV-2019_70_RIGHT	12
              nCoV-2019_71_LEFT	451
              nCoV-2019_71_RIGHT	630
              nCoV-2019_72_LEFT	38
              nCoV-2019_72_RIGHT	7
              nCoV-2019_73_LEFT	91
              nCoV-2019_73_RIGHT	58
              nCoV-2019_74_LEFT	0
              nCoV-2019_74_RIGHT	695
              nCoV-2019_75_LEFT	10352
              nCoV-2019_75_RIGHT	7650
              nCoV-2019_76_LEFT	2
              nCoV-2019_76_LEFT_alt3	97
              nCoV-2019_76_RIGHT	18
              nCoV-2019_76_RIGHT_alt0	44
              nCoV-2019_77_LEFT	6745
              nCoV-2019_77_RIGHT	12194
              nCoV-2019_78_LEFT	93
              nCoV-2019_78_RIGHT	10
              nCoV-2019_79_LEFT	221
              nCoV-2019_79_RIGHT	209
              nCoV-2019_80_LEFT	18
              nCoV-2019_80_RIGHT	6
              nCoV-2019_81_LEFT	10
              nCoV-2019_81_RIGHT	12
              nCoV-2019_82_LEFT	24
              nCoV-2019_82_RIGHT	13
              nCoV-2019_83_LEFT	23
              nCoV-2019_83_RIGHT	0
              nCoV-2019_84_LEFT	16
              nCoV-2019_84_RIGHT	18
              nCoV-2019_85_LEFT	7
              nCoV-2019_85_RIGHT	0
              nCoV-2019_86_LEFT	24
              nCoV-2019_86_RIGHT	45
              nCoV-2019_87_LEFT	9
              nCoV-2019_87_RIGHT	14
              nCoV-2019_88_LEFT	20
              nCoV-2019_88_RIGHT	36
              nCoV-2019_89_LEFT	2
              nCoV-2019_89_LEFT_alt2	6
              nCoV-2019_89_RIGHT	0
              nCoV-2019_89_RIGHT_alt4	13
              nCoV-2019_90_LEFT	21
              nCoV-2019_90_RIGHT	20
              nCoV-2019_91_LEFT	41
              nCoV-2019_91_RIGHT	24
              nCoV-2019_92_LEFT	25
              nCoV-2019_92_RIGHT	22
              nCoV-2019_93_LEFT	33
              nCoV-2019_93_RIGHT	38
              nCoV-2019_94_LEFT	72
              nCoV-2019_94_RIGHT	18
              nCoV-2019_95_LEFT	4
              nCoV-2019_95_RIGHT	1
              nCoV-2019_96_LEFT	0
              nCoV-2019_96_RIGHT	0
              nCoV-2019_97_LEFT	7
              nCoV-2019_97_RIGHT	24
              nCoV-2019_98_LEFT	48
              nCoV-2019_98_RIGHT	28
              
              Trimmed primers from 23.05% (90166) of reads.
              0% (0) of reads were quality trimmed below the minimum length of 1 bp and were not written to file.
              76.8% (300461) of reads started outside of primer regions. Since the -e flag was given, these reads were written to file.
              0.15% (604) reads were ignored because they did not fall within an amplicon
              13.41% (52454) of reads had their insert size smaller than their read length
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "bam"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              amplicons {"__current_case__": 1, "amplicon_info": {"values": [{"id": 175, "src": "hda"}]}, "filter_by": "yes"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              inc_primers true
              min_len "1"
              min_qual "0"
              primer {"__current_case__": 0, "input_bed": {"values": [{"id": 174, "src": "hda"}]}, "source": "history"}
              primer_pos_wiggle "0"
              window_width "4"
      • Step 18: toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' reference.fa && lofreq faidx reference.fa 2>&1 || echo "Error running samtools faidx for indexing fasta reference for lofreq" >&2 &&  ln -s '/tmp/tmpjh25ezu9/files/6/4/5/dataset_645ceb55-4daa-4c2a-84c7-e27f6f7249e8.dat' reads.bam && ln -s -f '/tmp/tmpjh25ezu9/files/_metadata_files/6/f/d/metadata_6fd7e138-102d-4f81-ba60-051b42aecbcc.dat' reads.bam.bai &&   lofreq call-parallel --pp-threads ${GALAXY_SLOTS:-1} --verbose  --ref 'reference.fa' --out variants.vcf --call-indels   --min-cov 5 --max-depth 1000000  --min-bq 30 --min-alt-bq 30    --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0  --sig 0.0005 --bonf dynamic --no-default-filter  reads.bam 2>&1  || (tool_exit_code=$? && cat "$TMPDIR/lofreq2_call_parallel*/*.log" 1>&2 && exit $tool_exit_code)

            Exit Code:

            • 0

            Standard Output:

            • INFO [2023-11-21 22:18:09,046]: Using 1 threads with following basic args: lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter reads.bam
              
              INFO [2023-11-21 22:18:09,049]: Adding 3 commands to mp-pool
              Number of substitution tests performed: 10101
              Number of indel tests performed: 2319
              INFO [2023-11-21 22:20:09,313]: Executing lofreq filter -i /tmp/tmpjh25ezu9/tmp/lofreq2_call_paralleljz94xt5b/concat.vcf.gz -o variants.vcf --no-defaults --snvqual-thresh 73 --indelqual-thresh 67
              
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              call_control {"__current_case__": 1, "align_quals": {"alnqual": {"__current_case__": 0, "alnqual_choice": {"__current_case__": 1, "alnquals_to_use": "", "extended_baq": true}, "use_alnqual": ""}}, "bc_quals": {"alt_bq": {"__current_case__": 0, "modify": ""}, "min_alt_bq": "30", "min_bq": "30"}, "coverage": {"max_depth": "1000000", "min_cov": "5"}, "joint_qual": {"def_alt_jq": "0", "min_alt_jq": "0", "min_jq": "0"}, "map_quals": {"min_mq": "20", "use_mq": {"__current_case__": 0, "max_mq": "255", "no_mq": ""}}, "pe": {"use_orphan": false}, "set_call_options": "yes", "source_qual": {"use_src_qual": {"__current_case__": 0, "src_qual": ""}}}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_control {"__current_case__": 3, "bonf": "0", "filter_type": "set_custom", "others": false, "sig": "0.0005"}
              reference_source {"__current_case__": 1, "ref": {"values": [{"id": 173, "src": "hda"}]}, "ref_selector": "history"}
              regions {"__current_case__": 0, "restrict_to_region": "genome"}
              variant_types "--call-indels"
      • Step 19: toolshed.g2.bx.psu.edu/repos/iuc/qualimap_bamqc/qualimap_bamqc/2.2.2d+galaxy3:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • export JAVA_OPTS="-Djava.awt.headless=true -Xmx${GALAXY_MEMORY_MB:-1024}m" &&    ln -s '/tmp/tmpjh25ezu9/files/6/4/5/dataset_645ceb55-4daa-4c2a-84c7-e27f6f7249e8.dat' 'SRR11578257' &&  qualimap bamqc -bam 'SRR11578257' -outdir results -outformat html --collect-overlap-pairs -nw 400 --paint-chromosome-limits -hm 3  --skip-duplicated --skip-dup-mode 0 -nt ${GALAXY_SLOTS:-1} &&   sed 's|images_qualimapReport/||g;s|css/||g' results/qualimapReport.html > '/tmp/tmpjh25ezu9/job_working_directory/000/100/outputs/dataset_52b1d5a1-0dca-4d75-90de-1cd12f4ec865.dat' && mkdir '/tmp/tmpjh25ezu9/job_working_directory/000/100/outputs/dataset_52b1d5a1-0dca-4d75-90de-1cd12f4ec865_files' && mv results/css/*.css '/tmp/tmpjh25ezu9/job_working_directory/000/100/outputs/dataset_52b1d5a1-0dca-4d75-90de-1cd12f4ec865_files' && mv results/css/*.png '/tmp/tmpjh25ezu9/job_working_directory/000/100/outputs/dataset_52b1d5a1-0dca-4d75-90de-1cd12f4ec865_files' && if [ -d results/images_qualimapReport ]; then mv results/images_qualimapReport/* '/tmp/tmpjh25ezu9/job_working_directory/000/100/outputs/dataset_52b1d5a1-0dca-4d75-90de-1cd12f4ec865_files' && for file in $(ls -A results/raw_data_qualimapReport); do mv "results/raw_data_qualimapReport/$file" `echo "results/$file" | sed 's/(//;s/)//'`; done fi && mv results/genome_results.txt results/summary_report.txt

            Exit Code:

            • 0

            Standard Output:

            • Java memory size is set to 1200M
              Launching application...
              
              detected environment java options -Djava.awt.headless=true -Xmx1024m
              QualiMap v.2.2.2-dev
              Built on 2019-11-11 14:05
              
              Selected tool: bamqc
              Available memory (Mb): 262
              Max memory (Mb): 1073
              Starting bam qc....
              Loading sam header...
              Loading locator...
              Loading reference...
              Only flagged duplicate alignments will be skipped...
              Number of windows: 400, effective number of windows: 399
              Chunk of reads size: 1000
              Number of threads: 1
              Processed 50 out of 399 windows...
              Processed 100 out of 399 windows...
              Processed 150 out of 399 windows...
              Processed 200 out of 399 windows...
              Processed 250 out of 399 windows...
              Processed 300 out of 399 windows...
              Processed 350 out of 399 windows...
              Total processed windows:399
              Number of reads: 390536
              Number of valid reads: 390627
              Number of correct strand reads:0
              
              Inside of regions...
              Num mapped reads: 390536
              Num mapped first of pair: 195268
              Num mapped second of pair: 195268
              Num singletons: 0
              Time taken to analyze reads: 8
              Computing descriptors...
              numberOfMappedBases: 55225999
              referenceSize: 29903
              numberOfSequencedBases: 55215868
              numberOfAs: 16872533
              Computing per chromosome statistics...
              Computing histograms...
              Overall analysis time: 8
              end of bam qc
              Computing report...
              Writing HTML report...
              HTML report created successfully
              
              Finished
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "bam"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              duplicate_skipping ["0"]
              per_base_coverage false
              plot_specific {"genome_gc_distr": null, "homopolymer_size": "3", "n_bins": "400", "paint_chromosome_limits": true}
              stats_regions {"__current_case__": 0, "region_select": "all"}
      • Step 20: toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_filter/4.3+t.galaxy1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • SnpSift -Xmx6G filter -f '/tmp/tmpjh25ezu9/files/9/0/c/dataset_90c0ad76-c990-4a9d-bd84-37e639a26b67.dat' -e '/tmp/tmpjh25ezu9/job_working_directory/000/101/configs/tmpl9vpnku2' > '/tmp/tmpjh25ezu9/job_working_directory/000/101/outputs/dataset_95e164b5-0956-48a8-bfbf-0b8ace61757a.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_expression {"__current_case__": 0, "expr": "( ( DP4[2] + DP4[3] ) >= ( 0.1 * DP ) ) & ( ( DP4[2] + DP4[3] ) <= ( 1 * DP ) )", "type": "simple"}
              filtering {"__current_case__": 0, "mode": "entries"}
              inverse false
      • Step 3: ARTIC primer BED:

        • step_state: scheduled
      • Step 21: toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_filter/4.3+t.galaxy1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • SnpSift -Xmx6G filter -f '/tmp/tmpjh25ezu9/files/9/0/c/dataset_90c0ad76-c990-4a9d-bd84-37e639a26b67.dat' -e '/tmp/tmpjh25ezu9/job_working_directory/000/102/configs/tmpn4u5shbv' > '/tmp/tmpjh25ezu9/job_working_directory/000/102/outputs/dataset_e1b55967-e85b-46f3-ae94-53e10266bca5.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_expression {"__current_case__": 0, "expr": "( DP > 1 ) & ( ( AF * DP ) >= ( 10 - 0.5 ) )", "type": "simple"}
              filtering {"__current_case__": 0, "mode": "entries"}
              inverse false
      • Step 22: __FILTER_FAILED_DATASETS__:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              input {"values": [{"id": 161, "src": "dce"}]}
      • Step 23: toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/ivar_removereads/1.3.1+galaxy2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • cp '/tmp/tmpjh25ezu9/files/0/f/e/dataset_0fe859b3-49b9-45e8-92ca-f5ed526f76a8.dat' binding_sites.bed && python '/tmp/shed_dir/toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/8d36959b000d/ivar_removereads/sanitize_bed.py' binding_sites.bed && ln -s '/tmp/tmpjh25ezu9/files/2/d/b/dataset_2dbd7a96-50ca-4a34-9548-be57064cdc4f.dat' amplicon_info.tsv && ivar getmasked -i '/tmp/tmpjh25ezu9/files/9/5/e/dataset_95e164b5-0956-48a8-bfbf-0b8ace61757a.dat' -b binding_sites.bed -f amplicon_info.tsv -p masked_primers &&  python '/tmp/shed_dir/toolshed.g2.bx.psu.edu/repos/iuc/ivar_removereads/8d36959b000d/ivar_removereads/completemask.py' masked_primers.txt amplicon_info.tsv && ln -s '/tmp/tmpjh25ezu9/files/6/4/5/dataset_645ceb55-4daa-4c2a-84c7-e27f6f7249e8.dat' sorted.bam && ln -s '/tmp/tmpjh25ezu9/files/_metadata_files/6/f/d/metadata_6fd7e138-102d-4f81-ba60-051b42aecbcc.dat' sorted.bam.bai &&  ivar removereads -i sorted.bam -b binding_sites.bed -p removed_reads.bam -t masked_primers.txt

            Exit Code:

            • 0

            Standard Output:

            • Found 218 primers in BED file
              Primer pair for nCoV-2019_7_RIGHT not found in BED file.
              Primer pair for nCoV-2019_9_LEFT_alt4 not found in BED file.
              Primer pair for nCoV-2019_9_RIGHT not found in BED file.
              Primer pair for nCoV-2019_11_RIGHT not found in BED file.
              Primer pair for nCoV-2019_14_RIGHT not found in BED file.
              Primer pair for nCoV-2019_18_LEFT_alt2 not found in BED file.
              Primer pair for nCoV-2019_18_RIGHT not found in BED file.
              Primer pair for nCoV-2019_20_RIGHT not found in BED file.
              Primer pair for nCoV-2019_21_RIGHT not found in BED file.
              Primer pair for nCoV-2019_23_RIGHT not found in BED file.
              Primer pair for nCoV-2019_44_RIGHT not found in BED file.
              Primer pair for nCoV-2019_46_LEFT_alt1 not found in BED file.
              Primer pair for nCoV-2019_46_RIGHT not found in BED file.
              Primer pair for nCoV-2019_50_RIGHT not found in BED file.
              Primer pair for nCoV-2019_76_RIGHT not found in BED file.
              Primer pair for nCoV-2019_78_RIGHT not found in BED file.
              Primer pair for nCoV-2019_89_RIGHT not found in BED file.
              Primer pair for nCoV-2019_91_RIGHT not found in BED file.
              nCoV-2019_6_LEFT
              nCoV-2019_58_RIGHT
              nCoV-2019_6_LEFT	nCoV-2019_6_RIGHT	nCoV-2019_58_RIGHT	nCoV-2019_58_LEFT
              
              Removing reads primed with any of:
              nCoV-2019_58_LEFT	nCoV-2019_58_RIGHT	nCoV-2019_6_LEFT	nCoV-2019_6_RIGHT
              Found 218 primers in BED file
              Writing to removed_reads.bam
              Number of references: 1
              Reference Name: NC_045512.2
              Reference Length: 29903
              Using Region: NC_045512.2
              Sorted By Coordinate
              Results:
              58 reads were removed.
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "bam"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              amplicons {"__current_case__": 1, "amplicon_info": {"values": [{"id": 175, "src": "hda"}]}, "computed": "no"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
      • Step 24: __FLATTEN__:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Job Parameters:

            • Job parameter Parameter value
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              input {"values": [{"id": 103, "src": "hdca"}]}
              join_identifier "_"
      • Step 25: toolshed.g2.bx.psu.edu/repos/iuc/lofreq_call/lofreq_call/2.1.5+galaxy1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' reference.fa && lofreq faidx reference.fa 2>&1 || echo "Error running samtools faidx for indexing fasta reference for lofreq" >&2 &&  ln -s '/tmp/tmpjh25ezu9/files/7/7/2/dataset_77282b34-82b1-45a7-9ddd-403f6f5b32e6.dat' reads.bam && ln -s -f '/tmp/tmpjh25ezu9/files/_metadata_files/c/6/c/metadata_c6c62ffa-f967-4915-9105-87881f18c526.dat' reads.bam.bai &&   lofreq call-parallel --pp-threads ${GALAXY_SLOTS:-1} --verbose  --ref 'reference.fa' --out variants.vcf --call-indels   --min-cov 5 --max-depth 1000000  --min-bq 30 --min-alt-bq 30    --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0  --sig 0.0005 --bonf dynamic --no-default-filter  reads.bam 2>&1  || (tool_exit_code=$? && cat "$TMPDIR/lofreq2_call_parallel*/*.log" 1>&2 && exit $tool_exit_code)

            Exit Code:

            • 0

            Standard Output:

            • INFO [2023-11-21 22:20:46,639]: Using 1 threads with following basic args: lofreq call --verbose --ref reference.fa --call-indels --min-cov 5 --max-depth 1000000 --min-bq 30 --min-alt-bq 30 --min-mq 20 --max-mq 255 --min-jq 0 --min-alt-jq 0 --def-alt-jq 0 --sig 0.0005 --bonf dynamic --no-default-filter reads.bam
              
              INFO [2023-11-21 22:20:46,643]: Adding 3 commands to mp-pool
              Number of substitution tests performed: 10086
              Number of indel tests performed: 2317
              INFO [2023-11-21 22:22:29,146]: Executing lofreq filter -i /tmp/tmpjh25ezu9/tmp/lofreq2_call_parallelzct12k21/concat.vcf.gz -o variants.vcf --no-defaults --snvqual-thresh 73 --indelqual-thresh 67
              
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              call_control {"__current_case__": 1, "align_quals": {"alnqual": {"__current_case__": 0, "alnqual_choice": {"__current_case__": 1, "alnquals_to_use": "", "extended_baq": true}, "use_alnqual": ""}}, "bc_quals": {"alt_bq": {"__current_case__": 0, "modify": ""}, "min_alt_bq": "30", "min_bq": "30"}, "coverage": {"max_depth": "1000000", "min_cov": "5"}, "joint_qual": {"def_alt_jq": "0", "min_alt_jq": "0", "min_jq": "0"}, "map_quals": {"min_mq": "20", "use_mq": {"__current_case__": 0, "max_mq": "255", "no_mq": ""}}, "pe": {"use_orphan": false}, "set_call_options": "yes", "source_qual": {"use_src_qual": {"__current_case__": 0, "src_qual": ""}}}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_control {"__current_case__": 3, "bonf": "0", "filter_type": "set_custom", "others": false, "sig": "0.0005"}
              reference_source {"__current_case__": 1, "ref": {"values": [{"id": 173, "src": "hda"}]}, "ref_selector": "history"}
              regions {"__current_case__": 0, "restrict_to_region": "genome"}
              variant_types "--call-indels"
      • Step 26: toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • die() { echo "$@" 1>&2 ; exit 1; } &&  mkdir multiqc_WDir &&   mkdir multiqc_WDir/fastp_0 &&    ln -s '/tmp/tmpjh25ezu9/files/0/b/5/dataset_0b5037b3-1221-43cc-a16b-899cd53ba596.dat' 'multiqc_WDir/fastp_0/SRR11578257fastp.json' && grep -q "report_title" 'multiqc_WDir/fastp_0/SRR11578257fastp.json' || die "'report_title' or 'report_title' not found in the file" && mkdir multiqc_WDir/samtools_1 &&    mkdir 'multiqc_WDir/samtools_1/stats_0' &&      grep -q 'This file was produced by samtools stats' /tmp/tmpjh25ezu9/files/f/1/5/dataset_f15aaf73-bc37-4ef7-b293-14cadb50dff2.dat || die "Module 'samtools: 'This file was produced by samtools stats' not found in the file 'SRR11578257'" && ln -s '/tmp/tmpjh25ezu9/files/f/1/5/dataset_f15aaf73-bc37-4ef7-b293-14cadb50dff2.dat' 'multiqc_WDir/samtools_1/stats_0/SRR11578257'  &&    mkdir multiqc_WDir/qualimap_2 &&  sample="$(grep 'bam file = ' /tmp/tmpjh25ezu9/files/6/3/d/dataset_63d2098e-f62d-4386-8193-365d819c4ac3.dat | sed 's/bam file = //g' | sed 's: ::g')" && dir_name="multiqc_WDir/qualimap_2/${sample}" && mkdir -p ${dir_name} && filepath_1="${dir_name}/genome_results.txt" && ln -sf '/tmp/tmpjh25ezu9/files/6/3/d/dataset_63d2098e-f62d-4386-8193-365d819c4ac3.dat' ${filepath_1} && nested_dir_name="${dir_name}/raw_data_qualimapReport/" && mkdir -p ${nested_dir_name} && filepath_2="${nested_dir_name}/coverage_histogram.txt" && ln -sf '/tmp/tmpjh25ezu9/files/1/7/c/dataset_17c5987d-f649-469f-b937-663bf7bef4d0.dat' ${filepath_2} && nested_dir_name="${dir_name}/raw_data_qualimapReport/" && mkdir -p ${nested_dir_name} && filepath_3="${nested_dir_name}/mapped_reads_gc-content_distribution.txt" && ln -sf '/tmp/tmpjh25ezu9/files/9/d/4/dataset_9d41b983-1102-4602-a27b-244dd4a9bf7d.dat' ${filepath_3} &&   multiqc multiqc_WDir --filename 'report'    --export

            Exit Code:

            • 0

            Standard Error:

            •   /// MultiQC 🔍 | v1.11
              
              |           multiqc | MultiQC Version v1.17 now available!
              |           multiqc | Search path : /tmp/tmpjh25ezu9/job_working_directory/000/114/working/multiqc_WDir
              |          qualimap | Found 1 BamQC reports
              |          samtools | Found 1 stats reports
              |             fastp | Found 1 reports
              |           multiqc | Compressing plot data
              |           multiqc | Report      : report.html
              |           multiqc | Data        : report_data
              |           multiqc | Plots       : report_plots
              |           multiqc | MultiQC complete
              

            Standard Output:

            • |         searching | ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% 5/5  

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              comment ""
              dbkey "?"
              export true
              flat false
              results [{"__index__": 0, "software_cond": {"__current_case__": 7, "input": {"values": [{"id": 81, "src": "hdca"}]}, "software": "fastp"}}, {"__index__": 1, "software_cond": {"__current_case__": 24, "output": [{"__index__": 0, "type": {"__current_case__": 0, "input": {"values": [{"id": 85, "src": "hdca"}]}, "type": "stats"}}], "software": "samtools"}}, {"__index__": 2, "software_cond": {"__current_case__": 20, "input": {"values": [{"id": 104, "src": "hdca"}]}, "software": "qualimap"}}]
              saveLog false
              title ""
      • Step 27: toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.10:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • export BCFTOOLS_PLUGINS=`which bcftools | sed 's,bin/bcftools,libexec/bcftools,'`;     bgzip -c '/tmp/tmpjh25ezu9/files/9/0/c/dataset_90c0ad76-c990-4a9d-bd84-37e639a26b67.dat' > input.vcf.gz && bcftools index input.vcf.gz &&   bgzip -c "/tmp/tmpjh25ezu9/files/6/0/f/dataset_60faacd2-b01f-45a3-9d3f-af1033b5e96f.dat" > annotations.vcf.gz && bcftools index annotations.vcf.gz &&  bcftools annotate       --columns 'QUAL,INFO'  --annotations "annotations.vcf.gz"  --mark-sites "-AmpliconBias"                 --output-type 'v'   --threads ${GALAXY_SLOTS:-4}    input.vcf.gz  > '/tmp/tmpjh25ezu9/job_working_directory/000/105/outputs/dataset_8b14532b-cf6e-48c9-b8d3-7e8df0dc3d32.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              output_type "v"
              sec_annofile {"annofile": {"__current_case__": 1, "anno_fmt": "vcf", "annotations": {"values": [{"id": 179, "src": "dce"}]}}, "columns": "QUAL,INFO", "mark_sites": "-AmpliconBias", "set_id": ""}
              sec_annotate {"remove": "", "rename_chrs": null}
              sec_restrict {"collapse": null, "exclude": "", "include": "", "invert_samples": false, "invert_samples_file": false, "regions": {"__current_case__": 0, "regions_src": "__none__"}, "samples": "", "samples_file": null}
      • Step 28: toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_filter/4.3+t.galaxy1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • SnpSift -Xmx6G filter -f '/tmp/tmpjh25ezu9/files/6/0/f/dataset_60faacd2-b01f-45a3-9d3f-af1033b5e96f.dat' -e '/tmp/tmpjh25ezu9/job_working_directory/000/106/configs/tmpr3snuxo7' > '/tmp/tmpjh25ezu9/job_working_directory/000/106/outputs/dataset_532f1ae5-9e00-4900-93f9-9643266812f4.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_expression {"__current_case__": 0, "expr": "( DP > 1 ) & ( ( AF * DP ) >= ( 10 - 0.5 ) )", "type": "simple"}
              filtering {"__current_case__": 0, "mode": "entries"}
              inverse false
      • Step 29: toolshed.g2.bx.psu.edu/repos/devteam/vcfvcfintersect/vcfvcfintersect/1.0.0_rc3+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/6/5/1/dataset_65125b37-5426-4e33-ad32-e5c65ee4bcff.dat' 'localref.fa' &&  vcfintersect  -v -r 'localref.fa' -w "0" -i '/tmp/tmpjh25ezu9/files/5/3/2/dataset_532f1ae5-9e00-4900-93f9-9643266812f4.dat' '/tmp/tmpjh25ezu9/files/e/1/b/dataset_e1b55967-e85b-46f3-ae94-53e10266bca5.dat' > '/tmp/tmpjh25ezu9/job_working_directory/000/107/outputs/dataset_eefa6af7-e754-44b1-83ec-29e10aad8bc4.dat'

            Exit Code:

            • 0

            Standard Error:

            • index file localref.fa.fai not found, generating...
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              adv_options {"__current_case__": 0, "adv_options_selector": "no"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              invert true
              isect_union "-i"
              loci false
              reference_source {"__current_case__": 1, "ref_file": {"values": [{"id": 173, "src": "hda"}]}, "reference_source_selector": "history"}
              window_size "0"
      • Step 30: toolshed.g2.bx.psu.edu/repos/iuc/bcftools_annotate/bcftools_annotate/1.10:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • export BCFTOOLS_PLUGINS=`which bcftools | sed 's,bin/bcftools,libexec/bcftools,'`;     bgzip -c '/tmp/tmpjh25ezu9/files/8/b/1/dataset_8b14532b-cf6e-48c9-b8d3-7e8df0dc3d32.dat' > input.vcf.gz && bcftools index input.vcf.gz &&   bgzip -c "/tmp/tmpjh25ezu9/files/e/e/f/dataset_eefa6af7-e754-44b1-83ec-29e10aad8bc4.dat" > annotations.vcf.gz && bcftools index annotations.vcf.gz &&  bcftools annotate       --columns 'QUAL,INFO'  --annotations "annotations.vcf.gz"  --mark-sites "+AmpliconBias"                 --output-type 'v'   --threads ${GALAXY_SLOTS:-4}    input.vcf.gz  > '/tmp/tmpjh25ezu9/job_working_directory/000/108/outputs/dataset_afb4ac04-24ed-4feb-96bf-00c5263cc97c.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              output_type "v"
              sec_annofile {"annofile": {"__current_case__": 1, "anno_fmt": "vcf", "annotations": {"values": [{"id": 182, "src": "dce"}]}}, "columns": "QUAL,INFO", "mark_sites": "+AmpliconBias", "set_id": ""}
              sec_annotate {"remove": "", "rename_chrs": null}
              sec_restrict {"collapse": null, "exclude": "", "include": "", "invert_samples": false, "invert_samples_file": false, "regions": {"__current_case__": 0, "regions_src": "__none__"}, "samples": "", "samples_file": null}
      • Step 4: ARTIC primers to amplicon assignments:

        • step_state: scheduled
      • Step 31: toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.2:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • sed -r --sandbox -e 's/^##INFO=<ID=AmpliconBias,.+$/##INFO=<ID=AmpliconBias,Number=0,Type=Flag,Description="Indicates that the AF value of the variant could not be corrected for potential amplicon bias.">/g' -e 's/^##INFO=<ID=AF,.+$/##INFO=<ID=AF,Number=1,Type=Float,Description="Lofreq Allele Frequency; Fraction of variant-supporting bases with q > --min-bq among all bases at the site">/g' '/tmp/tmpjh25ezu9/files/a/f/b/dataset_afb4ac04-24ed-4feb-96bf-00c5263cc97c.dat' > '/tmp/tmpjh25ezu9/job_working_directory/000/109/outputs/dataset_2b6b0315-3f83-4e60-9650-eea0be108e37.dat'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              replacements [{"__index__": 0, "find_pattern": "^##INFO=<ID=AmpliconBias,.+$", "replace_pattern": "##INFO=<ID=AmpliconBias,Number=0,Type=Flag,Description=\"Indicates that the AF value of the variant could not be corrected for potential amplicon bias.\">"}, {"__index__": 1, "find_pattern": "^##INFO=<ID=AF,.+$", "replace_pattern": "##INFO=<ID=AF,Number=1,Type=Float,Description=\"Lofreq Allele Frequency; Fraction of variant-supporting bases with q > --min-bq among all bases at the site\">"}]
      • Step 32: SnpEff eff covid19 version:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • snpEff -Xmx${GALAXY_MEMORY_MB:-8192}m eff -i vcf -o vcf -upDownStreamLen 0 -formatEff -classic -no-downstream -no-intergenic -no-upstream                  -stats '/tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293.dat' -noLog  NC_045512.2  '/tmp/tmpjh25ezu9/files/2/b/6/dataset_2b6b0315-3f83-4e60-9650-eea0be108e37.dat' > '/tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_ee871c1b-e56d-4251-b99d-8f5df0f7e10d.dat'  && mkdir '/tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293_files' && mv '/tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293.dat.genes.txt' '/tmp/tmpjh25ezu9/job_working_directory/000/110/outputs/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293_files/dataset_18c71d8f-d9a2-4ff3-8652-a37be54a7293.dat.genes.txt'

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "vcf"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              annotations ["-formatEff", "-classic"]
              chr ""
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              csvStats false
              dbkey "?"
              filter {"__current_case__": 0, "specificEffects": "no"}
              filterOut ["-no-downstream", "-no-intergenic", "-no-upstream"]
              generate_stats true
              genome_version "NC_045512.2"
              inputFormat "vcf"
              intervals None
              noLog true
              offset "default"
              outputConditional {"__current_case__": 0, "outputFormat": "vcf"}
              transcripts None
              udLength "0"
      • Step 33: toolshed.g2.bx.psu.edu/repos/iuc/lofreq_filter/lofreq_filter/2.1.5+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • lofreq filter -i /tmp/tmpjh25ezu9/files/e/e/8/dataset_ee871c1b-e56d-4251-b99d-8f5df0f7e10d.dat --no-defaults --verbose --print-all  -v 0 -V 0 -a 0.0 -A 0.0 -b fdr -c 0.001   -o filtered.vcf

            Exit Code:

            • 0

            Standard Error:

            • Skipping default settings
              At least one type of multiple testing correction requested. Doing first pass of vcf
              MTC application completed
              Successful exit.
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              af {"af_max": "0.0", "af_min": "0.0"}
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              coverage {"cov_max": "0", "cov_min": "0"}
              dbkey "?"
              filter_by_type {"__current_case__": 0, "keep_only": "", "qual": {"indelqual_filter": {"__current_case__": 0, "indelqual": "no"}, "snvqual_filter": {"__current_case__": 0, "snvqual": "no"}}}
              flag_or_drop "--print-all"
              sb {"sb_filter": {"__current_case__": 2, "sb_alpha": "0.001", "sb_compound": true, "sb_indels": false, "sb_mtc": "fdr", "strand_bias": "mtc"}}
      • Step 5: Read removal minimum AF:

        • step_state: scheduled
      • Step 6: Read removal maximum AF:

        • step_state: scheduled
      • Step 7: Minimum DP required after amplicon bias correction:

        • step_state: scheduled
      • Step 8: Minimum DP_ALT required after amplicon bias correction:

        • step_state: scheduled
      • Step 9: toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/0.23.2+galaxy0:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • ln -s '/tmp/tmpjh25ezu9/files/5/1/9/dataset_519e3c79-36ec-4151-8d0d-1609db8f8e58.dat' 'SRR11578257.fastq.gz' && ln -s '/tmp/tmpjh25ezu9/files/0/7/e/dataset_07e930ec-bb9a-413d-ab9e-5f2c31c64220.dat' 'SRR11578257_R2.fastq.gz' &&    fastp  --thread ${GALAXY_SLOTS:-1} --report_title 'fastp report for SRR11578257.fastq.gz'   -i 'SRR11578257.fastq.gz' -o first.fastq.gz  -I 'SRR11578257_R2.fastq.gz' -O second.fastq.gz       --detect_adapter_for_pe                                          &&  mv first.fastq.gz '/tmp/tmpjh25ezu9/job_working_directory/000/90/outputs/dataset_6677d982-521c-43d0-bea8-238c3c90ce3a.dat' && mv second.fastq.gz '/tmp/tmpjh25ezu9/job_working_directory/000/90/outputs/dataset_bd98e280-1ab3-4214-b195-3d88db763ac0.dat'

            Exit Code:

            • 0

            Standard Error:

            • Detecting adapter sequence for read1...
              ACCTTAGAATCAAGATTGTTAGAATTCCAAGCTATAACGCAGCCTGTAAAATCATCTGGT
              
              Detecting adapter sequence for read2...
              No adapter detected for read2
              
              Read1 before filtering:
              total reads: 201367
              total bases: 29302687
              Q20 bases: 28139796(96.0315%)
              Q30 bases: 27281893(93.1037%)
              
              Read2 before filtering:
              total reads: 201367
              total bases: 29398146
              Q20 bases: 27778430(94.4904%)
              Q30 bases: 26748339(90.9865%)
              
              Read1 after filtering:
              total reads: 195579
              total bases: 28439132
              Q20 bases: 27427010(96.4411%)
              Q30 bases: 26623439(93.6155%)
              
              Read2 after filtering:
              total reads: 195579
              total bases: 28450670
              Q20 bases: 27054927(95.0942%)
              Q30 bases: 26092568(91.7116%)
              
              Filtering result:
              reads passed filter: 391158
              reads failed due to low quality: 5634
              reads failed due to too many N: 1164
              reads failed due to too short: 4778
              reads with adapter trimmed: 18954
              bases trimmed due to adapters: 261387
              
              Duplication rate: 20.2521%
              
              Insert size peak (evaluated by paired-end reads): 230
              
              JSON report: fastp.json
              HTML report: fastp.html
              
              fastp --thread 1 --report_title fastp report for SRR11578257.fastq.gz -i SRR11578257.fastq.gz -o first.fastq.gz -I SRR11578257_R2.fastq.gz -O second.fastq.gz --detect_adapter_for_pe 
              fastp v0.23.2, time used: 13 seconds
              

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "fastqsanger.gz"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              dbkey "?"
              filter_options {"length_filtering_options": {"disable_length_filtering": false, "length_limit": null, "length_required": null}, "low_complexity_filter": {"complexity_threshold": null, "enable_low_complexity_filter": false}, "quality_filtering_options": {"disable_quality_filtering": false, "n_base_limit": null, "qualified_quality_phred": null, "unqualified_percent_limit": null}}
              output_options {"report_html": true, "report_json": true}
              overrepresented_sequence_analysis {"overrepresentation_analysis": false, "overrepresentation_sampling": null}
              read_mod_options {"base_correction_options": {"correction": false}, "cutting_by_quality_options": {"cut_by_quality3": false, "cut_by_quality5": false, "cut_mean_quality": null, "cut_window_size": null}, "polyg_tail_trimming": {"__current_case__": 1, "poly_g_min_len": null, "trimming_select": ""}, "polyx_tail_trimming": {"__current_case__": 1, "polyx_trimming_select": ""}, "umi_processing": {"umi": false, "umi_len": null, "umi_loc": "", "umi_prefix": ""}}
              single_paired {"__current_case__": 2, "adapter_trimming_options": {"adapter_sequence1": "", "adapter_sequence2": "", "disable_adapter_trimming": false}, "global_trimming_options": {"trim_front1": null, "trim_front2": null, "trim_tail1": null, "trim_tail2": null}, "paired_input": {"values": [{"id": 146, "src": "dce"}]}, "single_paired_selector": "paired_collection"}
      • Step 10: toolshed.g2.bx.psu.edu/repos/iuc/compose_text_param/compose_text_param/0.1.1:

        • step_state: scheduled

        • Jobs
          • Job 1:

            • Job state is ok

            Command Line:

            • cd ../; python _evaluate_expression_.py

            Exit Code:

            • 0

            Job Parameters:

            • Job parameter Parameter value
              __input_ext "input"
              __workflow_invocation_uuid__ "6e14231d88bb11ee950431e435efaaa6"
              chromInfo "/tmp/tmpjh25ezu9/galaxy-dev/tool-data/shared/ucsc/chrom/?.len"
              components [{"__index__": 0, "param_type": {"__current_case__": 0, "component_value": "( ( DP4[2] + DP4[3] ) >= ( ", "select_param_type": "text"}}, {"__index__": 1, "param_type": {"__current_case__": 2, "component_value": "0.1", "select_param_type": "float"}}, {"__index__": 2, "param_type": {"__current_case__": 0, "component_value": " * DP ) ) & ( ( DP4[2] + DP4[3] ) <= ( ", "select_param_type": "text"}}, {"__index__": 3, "param_type": {"__current_case__": 2, "component_value": "1.0", "select_param_type": "float"}}, {"__index__": 4, "param_type": {"__current_case__": 0, "component_value": " * DP ) )", "select_param_type": "text"}}]
              dbkey "?"
    • Other invocation details
      • history_id

        • 95630f1d00be8709
      • history_state

        • ok
      • invocation_id

        • 2d11ea9f25d0a2a5
      • invocation_state

        • scheduled
      • workflow_id

        • 95630f1d00be8709

@lldelisle
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For pe-artic-variation the result is different from expected:

-NC_045512.2	802	.	A	G	74	PASS	DP=29038;AF=0.001825;SB=18;DP4=21564,6726,55,30;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G)
+NC_045512.2	802	.	A	G	74	PASS	DP=29046;AF=0.001825;SB=18;DP4=21572,6726,55,30;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|7096|ORF1ab|protein_coding|CODING|GU280_gp01|1|G),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009725297.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|179|ORF1ab|protein_coding|CODING|YP_009742608.1|1|G|WARNING_TRANSCRIPT_NO_STOP_CODON),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G179|4405|ORF1ab|protein_coding|CODING|GU280_gp01.2|1|G)

Should I allow more line diff or update the expected result?

@mvdbeek
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mvdbeek commented Nov 22, 2023

Looks like DP has increased slightly, I would assume that's a fix coming from a newer version ?? Let's update the test result ? (ping @wm75).

@lldelisle
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Here we are:

Test Results (powered by Planemo)

Test Summary

Test State Count
Total 19
Passed 19
Error 0
Failure 0
Skipped 0

@lldelisle
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@mvdbeek ready for review. This PR is only:

  • dockstore file modification and associated release in workflow and changelog
  • -test.yml to -tests.yml renaming of test files
  • change the revision_changeset of gmx_solvate
  • change of url for fastq SRR11578257 and SRR12447380

The workflows which required more changes are adressed into separated PR: #293 and #295

@mvdbeek
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mvdbeek commented Nov 23, 2023

😍 Awesome, you really rock!

@mvdbeek mvdbeek merged commit ea509c0 into galaxyproject:main Nov 23, 2023
9 of 10 checks passed
@lldelisle
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This is my Swiss influence. I like when thinks are straight and clear.

@lldelisle lldelisle deleted the add_authors_to_all branch November 23, 2023 08:37
@lldelisle
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(Because of the failing lint step we may need to force deployement no?)

@mvdbeek
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mvdbeek commented Nov 23, 2023

No, linting should be excluded from the deployment requirements: https://github.com/galaxyproject/iwc/blob/main/.github/workflows/workflow_test.yml#L122

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2 participants