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Microfluidic enzyme assays

The Fluidigm Biomark HD microfluidic qPCR system was adapted to carrying out enzyme kinetic studies using a amplex-red based assay. This repository contains data, code and figures used in the ACS Omega article found here: https://pubs.acs.org/doi/abs/10.1021/acsomega.0c04918 (pre-print available at https://www.biorxiv.org/content/10.1101/2020.09.18.303248v1). The code is free to use and modify under GPLv3, but we do appreciate of you cite our article if you find it useful.

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Project Organization

├── LICENSE
├── README.md                <- The top-level README for developers using this project.
├── code
│   ├── flex_six_first_test  <- Code to analyze the FlexSix data.
│   ├── flex_six_km_test     <- Code to analyze the FlexSix data.
│   ├── plate_km_test        <- Code to analyze the plate data.
│   ├── pub_figs             <- Code to generate color grid in Figure 1.
│   └── resorufin_std_curves <- Code to analyze standard curves in FlexSix chip and microplate.
│
├── data
│   ├── final                    <- Final output files of processed data.
│   │   ├── flex_six_first_test  <- The initial test, exploring detergents and BSA concentrations.
│   │   ├── flex_six_km_test     <- Michaelis-Menten kinetics carried out on the FlexSix chip.
│   │   ├── plate_km_test        <- Michaelis-Menten kinetics carried out in microplate.
│   │   └── resorufin_std_curves <- Standard curves obtained using resorufin.
│   │
│   ├── intermediate             <- Intermediate data that has been transformed.
│   │   ├── flex_six_first_test  <- The initial test, exploring detergents and BSA concentrations.
│   │   ├── flex_six_km_test     <- Michaelis-Menten kinetics carried out on the FlexSix chip.
│   │   ├── plate_km_test        <- Michaelis-Menten kinetics carried out in microplate.
│   │   └── resorufin_std_curves <- Standard curves obtained using resorufin.
│   │
│   └── raw_internal             <- The raw unmodified data.
│       ├── flex_six_first_test  <- The initial test, exploring detergents and BSA concentrations.
│       ├── flex_six_km_test     <- Michaelis-Menten kinetics carried out on the FlexSix chip.
│       └── resorufin_std_curves <- Standard curves obtained using resorufin.
│
├── doc                          <- Folder containing the pre-print article.
│
└── reports            
    └── figures                  <- Generated graphics and figures to be used in reporting

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Enzyme kinetic assays in the Fluidigm Biomark HD instrument

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