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updat readme for freebayes parameters. --max-coverage to --max-limit
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rezabehboudi committed Apr 12, 2024
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Expand Up @@ -206,7 +206,7 @@ samtools index minitagged_sorted.bam
You may wish to break this into multiple jobs such as 1 job per chromosome and merge after but the basic command is the following.
Requires [freebayes](https://github.com/ekg/freebayes) and add /path/to/freebayes/bin to your PATH
```
freebayes -f <reference_fasta> -iXu -C 2 -q 20 -n 3 -E 1 -m 30 --min-coverage 6 --max-coverage 100000 minitagged_sorted.bam
freebayes -f <reference_fasta> -iXu -C 2 -q 20 -n 3 -E 1 -m 30 --min-coverage 6 --limit-coverage 100000 minitagged_sorted.bam
```

### 3. Cell allele counting
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