sgrna

On-Target and Off-Target Scoring Algorithms for CRISPR gRNAs

CRISPR/Cas-directed HDR genome editing suite: finds+scores gRNA targets, generates donor DNAs, & produces optimal cPCR primer designs.

sg5 sgRNA Designer is a command-line tool for designing CRISPR sgRNAs targeting Drosophila melanogaster genes using the SpCas9/NGG system. Fetches gene sequences directly from the Ensembl REST API, scans both strands for NGG PAM sites, scores each candidate guide, and outputs a ranked list.

sgRNA Oligo Designer — generates IDT bulk order forms and annotated plasmid GenBank files for CRISPR sgRNA cloning and sequencing verification.

Scalarization-free multi-objective preference alignment for generative CRISPR sgRNA design — standard DPO over Pareto-dominance preferences. Reproducible seeded pipeline, publication figures, and reviewer responses. Runs in <0.6 GB VRAM.

Commensal-sparing CRISPR guide design — target one bacterium, spare the microbiome. Narrow-spectrum, strain-selective, in-browser, free.