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mesoSPIM_electronics_block_diagram
Fabian edited this page Feb 13, 2019
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Block diagram of the mesoSPIM electronics: The sCMOS camera, zoom servo, mechanical stages and filter wheel all have dedicated connections to the imaging computer (PC) whereas the waveform generation (ETL ramps, galvo signals, shutter control, and laser intensity control) is done via a National Instruments PCIe-1073 PXI chassis.
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants