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Copy file name to clipboardExpand all lines: README.md
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# 🚀 Features
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- Processing (`results/`)
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- Alignment of both single-end and paired-end reads in raw/unalignedBAM format with Bowtie2.
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- Alignment of both single-end and paired-end reads in raw/unaligned/unmapped [uBAM](https://gatk.broadinstitute.org/hc/en-us/articles/360035532132-uBAM-Unmapped-BAM-Format) format with Bowtie2.
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- Filtering using `samtools view` can be configured using [SAM Flags](https://broadinstitute.github.io/picard/explain-flags.html) (`SAM_flag`).
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- Peak calling with `MACS2`.
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- Duplicate handling can be configured using `macs2_keep_dup`.
Copy file name to clipboardExpand all lines: config/README.md
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- annotation.csv: CSV file consisting of one technical sequencing unit per row (i.e., one sample can include multiple sequencing units, hence mutliple rows) and 4 mandatory columns:
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- sample_name (first column!)
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- read_type: "single" or "paired".
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- bam_file: path to the raw/unalignedBAM file.
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- bam_file: path to the raw/unaligned/unmapped [uBAM](https://gatk.broadinstitute.org/hc/en-us/articles/360035532132-uBAM-Unmapped-BAM-Format) file.
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- pass_qc: number between 0 (not used for downstream steps e.g., quantification) and 1. Every sample with pass_qc>0 is included in the downstream quantification and annotation steps.
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- (optional) additional sample metadata columns can be added and indicated for inclusion in the report.
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