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splisoquant_visualize.py fails to recognize output file after v4.0.0 rename (read_assignments.tsv.gz → read_info.tsv.gz) #11

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@Leilingshan

Hi,
Starting from spl-IsoQuant v4.0.0, the output file read_assignments.tsv.gz has been renamed to read_info.tsv.gz with 10x Visium HD 3' ONT full-length spatial data or Stereo-seq ONT full-length data . As a result, splisoquant_visualize.py can no longer recognize read_assignments.tsv.gz.

In addition, would it be possible to generate an HTML report summarizing the alignment results? Ideally, similar to the reports produced by wf-single-cell/Cell Ranger, it would include metrics such as the alignment rate to the reference genome, the valid barcode rate, and the proportion of reads assigned to barcodes at the gene level and transcript level.

Looking forward to your reply. Thanks!
Lingshan

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  1. andrewprzh commented on Sep 17, 2026

    @andrewprzh
    Collaborator

    Dear @Leilingshan

    Thanks for the report and for the suggestion! So far you can find alignment/assignment stats in the log file.

    Unfortunately, visualizer was maintained by a collaborator who is not active at the moment, which causes some lack of testing. I'll fix it.

    Best
    Andrey

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