Correcting donor effects using Combat-seq

[annepureddy]

I was wondering whether ComBat-seq can be used to correct for donor variation. I’m analyzing differences between sorted cell types across multiple donors. My dataset includes samples from five donors, and the groups are paired, as each donor contributes all cell types. Currently, the clustering is primarily driven by the donor, and using the DESeq2 model ~ Donor + Celltype does not yield any statistically significant differences between cell types. I’d like to reduce donor-driven variation while preserving true biological differences between cell types.