Skip to content

Batch effect present for some biotypes after running CombatSeq #41

@kvn95ss

Description

@kvn95ss

I'm working with smallRNA data and performed batch control with Combat_Seq (bioconductor version).

I used the below command -

library('sva')
corrected_batch <- ComBat_seq(as.matrix(counts), batch = coldata$Small_Seq_Batch)
corrected_batch <- as.data.frame(corrected_batch)

Here's a MDS plot before correcting -
MDS_before_correction

And here's the after
MDS_after_correction

What could cause this? I tried separating the biotypes first then performing the batch correction, but the outcome was pretty much the same.

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions