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@lwaldron, in reply to this comment, I found that the
filter.by.expr = TRUE
argument was added to thedeANA
function on a date after the manuscript was submitted and published (May/March 2019) in this commit (July 2019). This argument removed many OTUs. I changed the argument to FALSE (this PR), so that the OTUs were retained, and I was able to reproduce some of the GSEA results reported in the paper.