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v.0.5.0 [2023-08-08]

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@vmikk vmikk released this 25 Aug 12:56
· 143 commits to main since this release
  • New seqstats sub-workflow (only dereplication, primer validation, and basic run stats)
  • Add SWARM clustering (Mahé et al., 2022 DOI:10.1093/bioinformatics/btab493)
  • Add post-clustering curation with LULU (Frøslev et al., 2017 DOI:doi.org/10.1038)
  • Add barcode validation step
  • Add SSU and LSU region-based output sequences
  • Add support for UNOISE-only output (without clustering)
  • Add merge_replicates parameter (Step-2) for merging or keeping separate sample replicates
  • Update Step-1 run summary (add homopolymer stats)
  • Deprecate taxonomy annotation workflow at Step-1
  • Fixed different extensions in demultiplexed input
  • Experimental: UNITE-style dereplication (allows query sequences to vary in length at 100% similarity)
  • Experimental: support of alternative alignment penalty scores (ITS-specific feature)