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get more sequences back from filtering 250->270 #5

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get more sequences back from filtering 250->270 #5

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2 changes: 1 addition & 1 deletion workshops/microbiome/README.md
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Expand Up @@ -135,7 +135,7 @@ There are some false positives from the merging step, some reads after merging a
In this step, we shall remove low quality amplicons. We will filter reads based on expected error rates, filter longer than expected amplicons, and convert the reads to fasta format.

cd ${WORKDIR}/qc/
vsearch --fastq_filter ${WORKDIR}/mergepairs/SRR5972053_merged.fastq --fastq_maxee 1 --fastq_maxlen 250 --fastaout SRR5972053_merged_qc.fasta
vsearch --fastq_filter ${WORKDIR}/mergepairs/SRR5972053_merged.fastq --fastq_maxee 1 --fastq_maxlen 270 --fastaout SRR5972053_merged_qc.fasta


**_Relabel_**\
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