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|`inputs`|`-U`| Path to one or more files to use as input. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-S`| (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Bowtie 2. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/bowtie-2#supported-additional-arguments) for more details. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Bowtie 2. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details. |
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|`database_name`|`-x`\*| (optional) Name of database to use for Bowtie 2 alignment. Defaults to `"GRCh38_noalt_as"` (human genome). |
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|`database_version`|`-x`\*| (optional) Version of database to use for Bowtie 2 alignment. Defaults to `"1"`. |
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|`is_async`|| Whether to run a job asynchronously. See [Async Runs](../../feature-reference/async-runs.md) for more. |
|`inputs`|`-i`| Path to one or more files to use as input. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-o`| (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Clustal Omega. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/clustal-omega#supported-additional-arguments) for more details. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Clustal Omega. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details. |
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|`is_async`|| Whether to run a job asynchronously. See [Async Runs](../../feature-reference/async-runs.md) for more. |
|`inputs`|`-q`| Path to one or more files to use as input. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-o`| (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`database_name`|`-d`\*| (optional) Name of database to use for RAPSearch2 alignment. Defaults to `"GRCh38"` (human genome). |
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|`database_version`|`-d`\*| (optional) Version of database to use for RAPSearch2 alignment. Defaults to `"1"`. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to RAPSearch2. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/rapsearch-2#supported-additional-arguments) for more details. |
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|`is_async`|| Whether to run a job asynchronously. See [Async Runs](../../feature-reference/async-runs.md) for more. |
|`inputs`|`-q`| Path to one or more files to use as input. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-o`| (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`database_name`|`-d`\*| (optional) Name of database to use for RAPSearch2 alignment. Defaults to `"GRCh38"` (human genome). |
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|`database_version`|`-d`\*| (optional) Version of database to use for RAPSearch2 alignment. Defaults to `"1"`. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to RAPSearch2. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details.|
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|`is_async`|| Whether to run a job asynchronously. See [Async Runs](../../feature-reference/async-runs.md) for more. |
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\*See the [Databases](#databases) section for more details.
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|`read_two`|`-2`| (optional) Path to R2 of paired-end short read input files. The file can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`long_reads`|`-l`| (optional) Path to the file containing long reads. The file can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-o`| (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Unicycler. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/unicycler#supported-additional-arguments) for more details. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Unicycler. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details. |
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|`is_async`|| Whether to run a job asynchronously. See [Async Runs](../../feature-reference/async-runs.md) for more. |
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|`kraken2_report`| Kraken 2 report file input | Path to Kraken 2 report file. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`|`-o` directory name | (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_primary_name`|`-o` file name | (Optional) Name of Bracken output file. Defaults to `"output.bracken"`. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Bracken. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/kraken-2#supported-additional-arguments) for more details. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Bracken. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details. |
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|`database_name`|`-d`| (optional) Name of database that was used for Kraken 2 alignment. Defaults to `"standard"`. |
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|`database_version`|`-d`| (optional) Version of database that was used for Kraken 2 alignment. Defaults to `"1"`. |
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|`remote_database_path`|`-d`| (optional) AWS S3 URI to a directory with your custom database that was used with Kraken 2 alignment. |
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-`--max_template_date`
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-`--model_preset`
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However, these should be specified via specific argument values in the function call, rather than through a generic `tool_args` argument (like other Toolchest tools). See [Function Arguments](https://docs.trytoolchest.com/docs/alphafold#function-arguments) for more details.
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However, these should be specified via specific argument values in the function call, rather than through a generic `tool_args` argument (like other Toolchest tools). See [Function Arguments](#function-arguments) for more details.
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|`read_one`|`--paired`, input file location | (optional) Path to R1 of paired-end read input files. The file can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`read_two`|`--paired`, input file location | (optional) Path to R2 of paired-end read input files. The file can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`output_path`| output file location | (optional) Path (directory) to where the output files will be downloaded. If omitted, skips download. The files can be a local or remote, see [Using Files](../../getting-started/using-files.md). |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Kraken 2. This should be a string of arguments like the command line. See [Supported Additional Arguments](https://docs.trytoolchest.com/docs/kraken-2#supported-additional-arguments) for more details. |
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|`tool_args`| all other arguments | (optional) Additional arguments to be passed to Kraken 2. This should be a string of arguments like the command line. See [Supported Additional Arguments](#supported-additional-arguments) for more details. |
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|`database_name`|`--db`| (optional) Name of database to use for Kraken 2 alignment. Defaults to `"standard"`. |
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|`database_version`|`-db`| (optional) Version of database to use for Kraken 2 alignment. Defaults to `"1"`. |
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|`remote_database_path`|`-db`| (optional) AWS S3 URI to a directory with your custom database. |
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