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Two-photon calcium imaging of cross-orientation suppression across cortical depth in macaque V1. 4,785 ROIs across 28 depths (140-518um) reveal laminar shift from facilitation to suppression, paralleled by gradients in spatial frequency preference, tuning width, and orientation selectivity. Mixed-effects models and bootstrap CIs included.
Use your suite2p output to enhance your calcium imaging recording 'blink'. Good for presentations where projector quality might be an issue. Can help you avoid having to say 'it looks good on my screen here you've gotta believe me'