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ngs derive

Clay McLeod edited this page Oct 10, 2022 · 6 revisions

ngs derive is the predecessor to the ngsderive command line tool. The goal of this subcommand is to provide a forensic analysis tool useful in backwards computing information from next-generation sequencing data. The following forensic analysis tools are supported or will be supported in the future:

Forensic Analysis Tool Description Implemented
ngs derive instrument Infer which Illumina instrument was used to generate the data by matching against known instrument and flowcell naming patterns. Each guess comes with a confidence score.
ngs derive strandedness Infer from the data whether RNA-Seq data was generated using a Stranded-Forward, Stranded-Reverse, or Unstranded protocol.
ngs derive readlen Compute the distribution of read lengths in the file and attempt to guess what the original read length of the experiment was.
ngs derive encoding Infers which encoding scheme was used to store PHRED scores as ASCII characters.
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