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I wrongly filed this issue on rust-htslib: rust-bio/rust-htslib#179
After a bit more digging, I now believe it belongs here. I've put together a small reproducer using the serverless-aws-rust-multi template, just adding an additional lambda function called htslib:
https://github.com/brainstorm/serverless-aws-rust-multi-fail-native-crate
There's a full explanation and error log on the README from the repo above, but TL;DR the lamda-rust docker container doesn't seem to compile/link the rust-htslib crate with C bindings well:
= note: /code/target/lambda/release/deps/librust_htslib-fc86895fccfb6420.rlib(rust_htslib-fc86895fccfb6420.rust_htslib.25lsx2fx-cgu.11.rcgu.o): In function `rust_htslib::bam::Reader::new::h7805fb2441edac2e':
rust_htslib.25lsx2fx-cgu.11:(.text._ZN11rust_htslib3bam6Reader3new17h7805fb2441edac2eE+0x4c): undefined reference to `sam_hdr_read'
/code/target/lambda/release/deps/librust_htslib-fc86895fccfb6420.rlib(rust_htslib-fc86895fccfb6420.rust_htslib.25lsx2fx-cgu.11.rcgu.o): In function `_$LT$rust_htslib..bam..Reader$u20$as$u20$core..ops..drop..Drop$GT$::drop::hc749ab5efb9aea68':
rust_htslib.25lsx2fx-cgu.11:(.text._ZN66_$LT$rust_htslib..bam..Reader$u20$as$u20$core..ops..drop..Drop$GT$4drop17hc749ab5efb9aea68E+0x5): undefined reference to `hts_close'
/code/target/lambda/release/deps/librust_htslib-fc86895fccfb6420.rlib(rust_htslib-fc86895fccfb6420.rust_htslib.25lsx2fx-cgu.11.rcgu.o): In function `rust_htslib::bam::hts_open::hac1f0f64c9261566':
rust_htslib.25lsx2fx-cgu.11:(.text._ZN11rust_htslib3bam8hts_open17hac1f0f64c9261566E+0xc3): undefined reference to `hts_open'
/code/target/lambda/release/deps/librust_htslib-fc86895fccfb6420.rlib(rust_htslib-fc86895fccfb6420.rust_htslib.25lsx2fx-cgu.11.rcgu.o): In function `_$LT$rust_htslib..bam..HeaderView$u20$as$u20$core..ops..drop..Drop$GT$::drop::h06ec3494656e04e9':
rust_htslib.25lsx2fx-cgu.11:(.text._ZN70_$LT$rust_htslib..bam..HeaderView$u20$as$u20$core..ops..drop..Drop$GT$4drop17h06ec3494656e04e9E+0xb): undefined reference to `bam_hdr_destroy'
collect2: error: ld returned 1 exit status
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