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How to set/conserve orig.ident in Seurat objects converted from combined loom files? #29

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chatterjee89 opened this issue Feb 13, 2020 · 0 comments

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@chatterjee89
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First of all, I'm really enjoying Seurat and all the new integration tools! Thanks a bunch, team!
Y'all are amazing!

I've been working with velocyto integration lately and while it works great on individual objects, how can I use it on combined files that otherwise I'd be integrating in Seurat? I did this, because I read somewhere that I should begin from a combined loom files and convert to Seurat. This works well, except I'm not sure how to set ident in each files before combining. Any help on that?

I want to be able to see the velocity on the clusters, but I would also want to know where the cells are coming from. Assuming that this can be done prior to combining, I realize that this maybe a loom related question, not Seurat. But I'm a biologist, not a computational person and I feel like there might be a very easy solution to this that you might still be able to help me with.

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