UniEMIR

UniEMIR WebUI

We developed a web-based user interface, which can be deployed on high-performance GPU servers. You can use the colab to run the Web UI or employ the following steps to run the web user interface locally.

1. Install Packages

conda create -n uniemir python=3.11.8
conda activate uniemir
pip install torch==2.2.1+cu121 torchvision==0.17.1+cu121 -f https://download.pytorch.org/whl/torch_stable.html
pip install -r requirements.txt

3. Run the Web User Interface

python app.py

Then, you can visit the web interface at http://127.0.0.1:7860/. We provide a video tutorial for UniEMIR web user interface.

UniEMIR Plugin

UniEMIR plugin is an out-of-the-box extensions for the ImageJ, allowing for the restoration of 2D or 3D electron microscopy images on personal computers with CPU or GPU processing capabilities. You can download UniEMIR ImageJ plugin at https://github.com/ryanhe312/UniEMIR/releases and unzip to the ImageJ folder (the one with ImageJ-win64.exe). Please also make sure that you have Visual Studio installed.

Users can simply open an image and run the plugin to obtain results. The plugin accepts 2 (xy) and 3 (zxy) dimensional images in uint8 data type. We provide a video tutorial for UniEMIR plugin.

Training and Testing

1. Prepare Datasets

All training and testing data involved in the experiments come from existing literatures. You can download our preprocessed data from the zenodo repository and unzip them into the corresponding folders. Or you can prepare the dataset according to the instruction. Then, please edit the dataset path in config/*.json.

2. Inferencing Model

You can run the following command to test UniEMIR performance on given dataset.

• Denoising:

python run.py  -p test -c config/UniEMIR-denoise.json -b 16 --gpu 0 --resume experiments/train_UniEMIR-denoise/checkpoint/300

• Super-resolution

python run.py  -p test -c config/UniEMIR-zoom.json -b 16 --gpu 0 --resume experiments/train_UniEMIR-zoom/checkpoint/300

• Isotropic Reconstruction

python run.py  -p test -c config/UniEMIR-isotropic.json -b 16 --gpu 0 --resume experiments/train_UniEMIR-isotropic/checkpoint/300

The metrics for each test image can further be calculated by calling python metric.py path/to/output/dir. For example, python metric.py experiments/test_UniEMIR-denoise_240713_172734.

3. Training Model

You can run the following command to pretrain and finetune UniEMIR models.

1. Pretraining model on multiple tasks:

python run.py -c config/UniEMIR.json -b 6 --gpu 0

2. Finetuning model on single task:

python run.py -c config/UniEMIR-denoise.json -b 6 --gpu 0 --resume experiments/train_UniEMIR/checkpoint/200
python run.py -c config/UniEMIR-zoom.json -b 6 --gpu 0 --resume experiments/train_UniEMIR/checkpoint/200
python run.py -c config/UniEMIR-isotropic.json -b 6 --gpu 0 --resume experiments/train_UniEMIR/checkpoint/200

4. Exporting model for ImageJ plugin

You can run the following command to build models for ImageJ plugin. Arguments can be super-resolution, denoising and isotropic_reconstruction.

python export.py super-resolution

And you can unzip the models under models folder in ImageJ root folder.

CITE

@software{he2024pushing,
  author = {Ruian He, Weimin Tan, Chenxi Ma and Bo Yan},
  doi = {10.5281/zenodo.12738837},
  title = {UniEMIR},
  url = {https://github.com/ryanhe312/UniEMIR},
  year = {2024}
}