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How to install EDTA? #497

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zyy36920 opened this issue Aug 23, 2024 · 9 comments
Closed

How to install EDTA? #497

zyy36920 opened this issue Aug 23, 2024 · 9 comments
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help wanted Extra attention is needed

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@zyy36920
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When I run the program EDTA, an error occurred during the initial process: Invalid value for shared scalar at /lustre/home/huli/micromamba/envs/edta/share/LTR_retriever/bin/LTR.identifier.pl line 114, line 4266. Can this error could be repaired or are there any metohd to deal with this problem?

@SwiftSeal
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Also experiencing since updating to latest commits on master and using conda environment specified in #489 (comment)

@FayeFang17
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May I ask how did you install EDTA?

@SwiftSeal
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For me, that was using the conda environment recommended in the comment I linked (the master branch env, not the EDTA2 dev one). I have pasted the resulting environment below. In the end I have opted to use docker://quay.io/biocontainers/edta:2.0.0--hdfd78af_0 which runs fine :) (although which needs to be unset prior to running due to BusyBox)

# Name                    Version                   Build  Channel
_libgcc_mutex             0.1                 conda_forge    conda-forge
_openmp_mutex             4.5                       2_gnu    conda-forge
_r-mutex                  1.0.1               anacondar_1    conda-forge
_sysroot_linux-64_curr_repodata_hack 3                   h69a702a_16    conda-forge
absl-py                   2.1.0              pyhd8ed1ab_0    conda-forge
aiohappyeyeballs          2.4.0              pyhd8ed1ab_0    conda-forge
aiohttp                   3.10.5          py310h5b4e0ec_0    conda-forge
aiosignal                 1.3.1              pyhd8ed1ab_0    conda-forge
alsa-lib                  1.2.8                h166bdaf_0    conda-forge
annosine2                 2.0.7              pyh7cba7a3_0    bioconda
arrow-cpp                 11.0.0           ha770c72_5_cpu    conda-forge
astunparse                1.6.3              pyhd8ed1ab_0    conda-forge
async-timeout             4.0.3              pyhd8ed1ab_0    conda-forge
attrs                     24.2.0             pyh71513ae_0    conda-forge
aws-c-auth                0.6.24               h84a1944_5    conda-forge
aws-c-cal                 0.5.20               hc60faf5_6    conda-forge
aws-c-common              0.8.11               h0b41bf4_0    conda-forge
aws-c-compression         0.2.16               h034cb4b_3    conda-forge
aws-c-event-stream        0.2.18               h75388cd_6    conda-forge
aws-c-http                0.7.4                hf084cc8_2    conda-forge
aws-c-io                  0.13.17              h10df833_2    conda-forge
aws-c-mqtt                0.8.6                hc41645a_6    conda-forge
aws-c-s3                  0.2.4                h1b8f470_3    conda-forge
aws-c-sdkutils            0.1.7                h034cb4b_3    conda-forge
aws-checksums             0.1.14               h034cb4b_3    conda-forge
aws-crt-cpp               0.19.7               h0073717_7    conda-forge
aws-sdk-cpp               1.10.57              h4707e7a_4    conda-forge
binutils_impl_linux-64    2.40                 ha1999f0_7    conda-forge
biopython                 1.84            py310hc51659f_0    conda-forge
blast                     2.16.0               hc155240_2    bioconda
blinker                   1.8.2              pyhd8ed1ab_0    conda-forge
bokeh                     3.5.2              pyhd8ed1ab_0    conda-forge
brotli                    1.0.9                h166bdaf_9    conda-forge
brotli-bin                1.0.9                h166bdaf_9    conda-forge
brotli-python             1.0.9           py310hd8f1fbe_9    conda-forge
bwidget                   1.9.14               ha770c72_1    conda-forge
bzip2                     1.0.8                h4bc722e_7    conda-forge
c-ares                    1.33.1               heb4867d_0    conda-forge
ca-certificates           2024.7.4             hbcca054_0    conda-forge
cached-property           1.5.2                hd8ed1ab_1    conda-forge
cached_property           1.5.2              pyha770c72_1    conda-forge
cachetools                5.5.0              pyhd8ed1ab_0    conda-forge
cairo                     1.16.0            ha61ee94_1014    conda-forge
cd-hit                    4.8.1               h43eeafb_10    bioconda
certifi                   2024.7.4           pyhd8ed1ab_0    conda-forge
cffi                      1.17.0          py310h2fdcea3_0    conda-forge
charset-normalizer        3.3.2              pyhd8ed1ab_0    conda-forge
click                     8.1.7           unix_pyh707e725_0    conda-forge
cloudpickle               3.0.0              pyhd8ed1ab_0    conda-forge
colorama                  0.4.6              pyhd8ed1ab_0    conda-forge
contourpy                 1.2.1           py310hd41b1e2_0    conda-forge
coreutils                 9.5                  hd590300_0    conda-forge
cryptography              43.0.0          py310h4909e49_0    conda-forge
curl                      8.1.2                h409715c_0    conda-forge
cycler                    0.12.1             pyhd8ed1ab_0    conda-forge
cytoolz                   0.12.3          py310h2372a71_0    conda-forge
dask                      2024.8.1           pyhd8ed1ab_0    conda-forge
dask-core                 2024.8.1           pyhd8ed1ab_0    conda-forge
dask-expr                 1.1.11             pyhd8ed1ab_0    conda-forge
dill                      0.3.8              pyhd8ed1ab_0    conda-forge
distributed               2024.8.1           pyhd8ed1ab_0    conda-forge
drmaa                     0.7.9                   py_1000    conda-forge
entrez-direct             22.4                 he881be0_0    bioconda
expat                     2.6.2                h59595ed_0    conda-forge
flatbuffers               22.12.06             hcb278e6_2    conda-forge
font-ttf-dejavu-sans-mono 2.37                 hab24e00_0    conda-forge
font-ttf-inconsolata      3.000                h77eed37_0    conda-forge
font-ttf-source-code-pro  2.038                h77eed37_0    conda-forge
font-ttf-ubuntu           0.83                 h77eed37_2    conda-forge
fontconfig                2.14.2               h14ed4e7_0    conda-forge
fonts-conda-ecosystem     1                             0    conda-forge
fonts-conda-forge         1                             0    conda-forge
fonttools                 4.53.1          py310h5b4e0ec_0    conda-forge
freetype                  2.12.1               h267a509_2    conda-forge
fribidi                   1.0.10               h36c2ea0_0    conda-forge
frozenlist                1.4.1           py310h2372a71_0    conda-forge
fsspec                    2024.6.1           pyhff2d567_0    conda-forge
gast                      0.4.0              pyh9f0ad1d_0    conda-forge
gawk                      5.3.0                ha916aea_0    conda-forge
gcc_impl_linux-64         14.1.0               h3c94d91_0    conda-forge
genericrepeatfinder       1.0.2                h4ac6f70_0    bioconda
genometools-genometools   1.6.4           py310h3db02ab_0    bioconda
gettext                   0.22.5               he02047a_3    conda-forge
gettext-tools             0.22.5               he02047a_3    conda-forge
gflags                    2.2.2             he1b5a44_1004    conda-forge
gfortran_impl_linux-64    14.1.0               he4a1faa_0    conda-forge
giflib                    5.2.2                hd590300_0    conda-forge
glob2                     0.7                        py_0    conda-forge
glog                      0.6.0                h6f12383_0    conda-forge
gmp                       6.3.0                hac33072_2    conda-forge
google-auth               2.34.0             pyhff2d567_0    conda-forge
google-auth-oauthlib      0.4.6              pyhd8ed1ab_0    conda-forge
google-pasta              0.2.0              pyhd8ed1ab_1    conda-forge
graphite2                 1.3.13            h59595ed_1003    conda-forge
grpcio                    1.51.1          py310h4a5735c_1    conda-forge
gsl                       2.7                  he838d99_0    conda-forge
gxx_impl_linux-64         14.1.0               h8d00ecb_0    conda-forge
h2                        4.1.0              pyhd8ed1ab_0    conda-forge
h5py                      3.9.0           nompi_py310hcca72df_101    conda-forge
harfbuzz                  6.0.0                h8e241bc_0    conda-forge
hdf5                      1.14.1          nompi_h4f84152_100    conda-forge
hmmer                     3.4                  hdbdd923_2    bioconda
hpack                     4.0.0              pyh9f0ad1d_0    conda-forge
hyperframe                6.0.1              pyhd8ed1ab_0    conda-forge
icu                       70.1                 h27087fc_0    conda-forge
idna                      3.8                pyhd8ed1ab_0    conda-forge
importlib-metadata        8.4.0              pyha770c72_0    conda-forge
importlib_metadata        8.4.0                hd8ed1ab_0    conda-forge
irf                       3.08                 hdbdd923_0    bioconda
isa-l                     2.31.0               h4bc722e_2    conda-forge
jinja2                    3.1.4              pyhd8ed1ab_0    conda-forge
joblib                    1.4.2              pyhd8ed1ab_0    conda-forge
jpeg                      9e                   h0b41bf4_3    conda-forge
k8                        0.2.5                hdcf5f25_4    bioconda
keras                     2.11.0             pyhd8ed1ab_0    conda-forge
keras-preprocessing       1.1.2              pyhd8ed1ab_0    conda-forge
kernel-headers_linux-64   3.10.0              h4a8ded7_16    conda-forge
keyutils                  1.6.1                h166bdaf_0    conda-forge
kiwisolver                1.4.5           py310hd41b1e2_1    conda-forge
krb5                      1.20.1               h81ceb04_0    conda-forge
lcms2                     2.15                 hfd0df8a_0    conda-forge
ld_impl_linux-64          2.40                 hf3520f5_7    conda-forge
lerc                      4.0.0                h27087fc_0    conda-forge
libabseil                 20220623.0      cxx17_h05df665_6    conda-forge
libaec                    1.1.3                h59595ed_0    conda-forge
libarrow                  11.0.0           h2ebd325_5_cpu    conda-forge
libasprintf               0.22.5               he8f35ee_3    conda-forge
libasprintf-devel         0.22.5               he8f35ee_3    conda-forge
libblas                   3.9.0           23_linux64_openblas    conda-forge
libbrotlicommon           1.0.9                h166bdaf_9    conda-forge
libbrotlidec              1.0.9                h166bdaf_9    conda-forge
libbrotlienc              1.0.9                h166bdaf_9    conda-forge
libcblas                  3.9.0           23_linux64_openblas    conda-forge
libcrc32c                 1.1.2                h9c3ff4c_0    conda-forge
libcups                   2.3.3                h36d4200_3    conda-forge
libcurl                   8.1.2                h409715c_0    conda-forge
libdeflate                1.17                 h0b41bf4_0    conda-forge
libedit                   3.1.20191231         he28a2e2_2    conda-forge
libev                     4.33                 hd590300_2    conda-forge
libevent                  2.1.10               h28343ad_4    conda-forge
libexpat                  2.6.2                h59595ed_0    conda-forge
libffi                    3.4.2                h7f98852_5    conda-forge
libgcc-devel_linux-64     14.1.0             h5d3d1c9_100    conda-forge
libgcc-ng                 14.1.0               h77fa898_0    conda-forge
libgd                     2.3.3                h5aea950_4    conda-forge
libgettextpo              0.22.5               he02047a_3    conda-forge
libgettextpo-devel        0.22.5               he02047a_3    conda-forge
libgfortran-ng            14.1.0               h69a702a_0    conda-forge
libgfortran5              14.1.0               hc5f4f2c_0    conda-forge
libglib                   2.78.1               hebfc3b9_0    conda-forge
libgomp                   14.1.0               h77fa898_0    conda-forge
libgoogle-cloud           2.7.0                h21dfe5b_1    conda-forge
libgrpc                   1.51.1               h4fad500_1    conda-forge
libiconv                  1.17                 hd590300_2    conda-forge
libidn2                   2.3.7                hd590300_0    conda-forge
liblapack                 3.9.0           23_linux64_openblas    conda-forge
libnghttp2                1.58.0               h47da74e_1    conda-forge
libnsl                    2.0.1                hd590300_0    conda-forge
libopenblas               0.3.27          pthreads_hac2b453_1    conda-forge
libpng                    1.6.43               h2797004_0    conda-forge
libprotobuf               3.21.12              hfc55251_2    conda-forge
libsanitizer              14.1.0               hcba0ae0_0    conda-forge
libsqlite                 3.46.0               hde9e2c9_0    conda-forge
libssh2                   1.11.0               h0841786_0    conda-forge
libstdcxx-devel_linux-64  14.1.0             h5d3d1c9_100    conda-forge
libstdcxx-ng              14.1.0               hc0a3c3a_0    conda-forge
libthrift                 0.18.0               h5e4af38_0    conda-forge
libtiff                   4.5.0                h6adf6a1_2    conda-forge
libunistring              0.9.10               h7f98852_0    conda-forge
libutf8proc               2.8.0                h166bdaf_0    conda-forge
libuuid                   2.38.1               h0b41bf4_0    conda-forge
libuv                     1.44.2               hd590300_1    conda-forge
libwebp                   1.2.4                h1daa5a0_1    conda-forge
libwebp-base              1.2.4                h166bdaf_0    conda-forge
libxcb                    1.13              h7f98852_1004    conda-forge
libxcrypt                 4.4.36               hd590300_1    conda-forge
libxml2                   2.10.3               hca2bb57_4    conda-forge
libzlib                   1.2.13               h4ab18f5_6    conda-forge
locket                    1.0.0              pyhd8ed1ab_0    conda-forge
ltr_finder                1.07                 h4ac6f70_3    bioconda
ltr_retriever             2.9.9                hdfd78af_0    bioconda
lz4                       4.3.3           py310h350c4a5_0    conda-forge
lz4-c                     1.9.4                hcb278e6_0    conda-forge
mafft                     7.526                h4bc722e_0    conda-forge
make                      4.4.1                hb9d3cd8_0    conda-forge
markdown                  3.6                pyhd8ed1ab_0    conda-forge
markupsafe                2.1.5           py310h2372a71_0    conda-forge
matplotlib-base           3.9.2           py310hf02ac8c_0    conda-forge
mdust                     2006.10.17           h031d066_6    bioconda
minimap2                  2.28                 he4a0461_2    bioconda
mpfr                      4.2.1                h38ae2d0_2    conda-forge
msgpack-python            1.0.8           py310h25c7140_0    conda-forge
multidict                 6.0.5           py310h2372a71_0    conda-forge
multiprocess              0.70.16         py310h2372a71_0    conda-forge
munkres                   1.1.4              pyh9f0ad1d_0    conda-forge
muscle                    5.1                  h4ac6f70_3    bioconda
mysql-connector-c         6.1.11            h659d440_1008    conda-forge
ncbi-vdb                  3.1.1                h4ac6f70_1    bioconda
ncurses                   6.5                  he02047a_1    conda-forge
ninja-nj                  0.98                 h4ac6f70_3    bioconda
nodejs                    18.15.0              h8d033a5_0    conda-forge
nseg                      1.0.1                h031d066_4    bioconda
numpy                     1.26.4          py310hb13e2d6_0    conda-forge
oauthlib                  3.2.2              pyhd8ed1ab_0    conda-forge
openjdk                   17.0.3               h58dac75_5    conda-forge
openjpeg                  2.5.0                hfec8fc6_2    conda-forge
openssl                   3.3.1                hb9d3cd8_3    conda-forge
opt_einsum                3.3.0              pyhc1e730c_2    conda-forge
orc                       1.8.2                hfdbbad2_2    conda-forge
packaging                 24.1               pyhd8ed1ab_0    conda-forge
pandas                    2.2.2           py310hf9f9076_1    conda-forge
pango                     1.50.14              hd33c08f_0    conda-forge
parquet-cpp               1.5.1                         2    conda-forge
partd                     1.4.2              pyhd8ed1ab_0    conda-forge
pbzip2                    1.1.13               h1fcc475_2    conda-forge
pcre                      8.45                 h9c3ff4c_0    conda-forge
pcre2                     10.40                hc3806b6_0    conda-forge
perl                      5.32.1          7_hd590300_perl5    conda-forge
perl-archive-tar          2.40            pl5321hdfd78af_0    bioconda
perl-base                 2.23            pl5321hd8ed1ab_0    conda-forge
perl-business-isbn        3.007           pl5321hd8ed1ab_0    conda-forge
perl-business-isbn-data   20210112.006    pl5321hd8ed1ab_0    conda-forge
perl-carp                 1.50            pl5321hd8ed1ab_0    conda-forge
perl-common-sense         3.75            pl5321hd8ed1ab_0    conda-forge
perl-compress-raw-bzip2   2.201           pl5321h166bdaf_0    conda-forge
perl-compress-raw-zlib    2.202           pl5321h166bdaf_0    conda-forge
perl-constant             1.33            pl5321hd8ed1ab_0    conda-forge
perl-devel-size           0.83            pl5321h031d066_4    bioconda
perl-digest-hmac          1.04            pl5321hdfd78af_0    bioconda
perl-digest-md5           2.58            pl5321h166bdaf_0    conda-forge
perl-encode               3.21            pl5321hd590300_0    conda-forge
perl-encode-locale        1.05            pl5321hdfd78af_7    bioconda
perl-exporter             5.74            pl5321hd8ed1ab_0    conda-forge
perl-exporter-tiny        1.002002        pl5321hd8ed1ab_0    conda-forge
perl-extutils-makemaker   7.70            pl5321hd8ed1ab_0    conda-forge
perl-file-listing         6.16            pl5321hdfd78af_0    bioconda
perl-file-path            2.18            pl5321hd8ed1ab_0    conda-forge
perl-file-spec            3.48_01         pl5321hdfd78af_2    bioconda
perl-file-temp            0.2304          pl5321hd8ed1ab_0    conda-forge
perl-file-which           1.24            pl5321hd8ed1ab_0    conda-forge
perl-gd                   2.76            pl5321h5b5514e_1    bioconda
perl-html-parser          3.81            pl5321h4ac6f70_1    bioconda
perl-html-tagset          3.20            pl5321hdfd78af_4    bioconda
perl-http-cookies         6.10            pl5321hdfd78af_0    bioconda
perl-http-daemon          6.16            pl5321hdfd78af_0    bioconda
perl-http-date            6.06            pl5321hdfd78af_0    bioconda
perl-http-message         6.36            pl5321hdfd78af_0    bioconda
perl-http-negotiate       6.01            pl5321hdfd78af_4    bioconda
perl-io-compress          2.201           pl5321hdbdd923_2    bioconda
perl-io-html              1.004           pl5321hdfd78af_0    bioconda
perl-io-socket-ssl        2.075           pl5321hd8ed1ab_0    conda-forge
perl-io-zlib              1.14            pl5321hdfd78af_0    bioconda
perl-json                 4.10            pl5321hdfd78af_1    bioconda
perl-json-xs              4.03            pl5321h4ac6f70_3    bioconda
perl-libwww-perl          6.67            pl5321hdfd78af_0    bioconda
perl-list-moreutils       0.430           pl5321hdfd78af_0    bioconda
perl-list-moreutils-xs    0.430           pl5321h031d066_2    bioconda
perl-lwp-mediatypes       6.04            pl5321hdfd78af_1    bioconda
perl-mime-base64          3.16            pl5321h166bdaf_0    conda-forge
perl-net-http             6.22            pl5321hdfd78af_0    bioconda
perl-net-ssleay           1.92            pl5321hf14f497_1    conda-forge
perl-ntlm                 1.09            pl5321hdfd78af_5    bioconda
perl-parent               0.241           pl5321hd8ed1ab_0    conda-forge
perl-pathtools            3.75            pl5321h166bdaf_0    conda-forge
perl-scalar-list-utils    1.63            pl5321h166bdaf_0    conda-forge
perl-socket               2.027           pl5321h031d066_4    bioconda
perl-storable             3.15            pl5321h166bdaf_0    conda-forge
perl-test-fatal           0.016           pl5321hdfd78af_0    bioconda
perl-test-simple          1.302191        pl5321hd8ed1ab_0    conda-forge
perl-test-warnings        0.031           pl5321ha770c72_0    conda-forge
perl-text-soundex         3.05            pl5321hd8ed1ab_1001    conda-forge
perl-time-local           1.35            pl5321hdfd78af_0    bioconda
perl-timedate             2.33            pl5321hdfd78af_2    bioconda
perl-try-tiny             0.32            pl5321ha770c72_0    conda-forge
perl-types-serialiser     1.01            pl5321hdfd78af_0    bioconda
perl-uri                  5.17            pl5321ha770c72_0    conda-forge
perl-url-encode           0.03            pl5321h9ee0642_0    bioconda
perl-www-robotrules       6.02            pl5321hdfd78af_4    bioconda
pigz                      2.8                  h2797004_0    conda-forge
pillow                    9.4.0           py310h023d228_1    conda-forge
pip                       24.2               pyhd8ed1ab_0    conda-forge
pixman                    0.43.2               h59595ed_0    conda-forge
pp                        1.6.4.4                    py_0    bioconda
protobuf                  4.21.12         py310heca2aa9_0    conda-forge
psutil                    6.0.0           py310hc51659f_0    conda-forge
pthread-stubs             0.4               h36c2ea0_1001    conda-forge
pyarrow                   11.0.0          py310h633f555_5_cpu    conda-forge
pyarrow-hotfix            0.6                pyhd8ed1ab_0    conda-forge
pyasn1                    0.6.0              pyhd8ed1ab_0    conda-forge
pyasn1-modules            0.4.0              pyhd8ed1ab_0    conda-forge
pycparser                 2.22               pyhd8ed1ab_0    conda-forge
pyjwt                     2.9.0              pyhd8ed1ab_1    conda-forge
pyopenssl                 24.2.1             pyhd8ed1ab_2    conda-forge
pyparsing                 3.1.4              pyhd8ed1ab_0    conda-forge
pysocks                   1.7.1              pyha2e5f31_6    conda-forge
python                    3.10.14         hd12c33a_0_cpython    conda-forge
python-dateutil           2.9.0              pyhd8ed1ab_0    conda-forge
python-flatbuffers        24.3.25            pyh59ac667_0    conda-forge
python-isal               1.7.0           py310h5b4e0ec_0    conda-forge
python-tzdata             2024.1             pyhd8ed1ab_0    conda-forge
python-zlib-ng            0.5.0           py310h6420a16_0    conda-forge
python_abi                3.10                    5_cp310    conda-forge
pytz                      2024.1             pyhd8ed1ab_0    conda-forge
pyu2f                     0.1.5              pyhd8ed1ab_0    conda-forge
pyyaml                    6.0.2           py310h5b4e0ec_0    conda-forge
qhull                     2020.2               h434a139_5    conda-forge
r-base                    4.2.3                ha7d60f8_0    conda-forge
r-cli                     3.6.3             r42ha18555a_0    conda-forge
r-colorspace              2.1_0             r42h57805ef_1    conda-forge
r-crayon                  1.5.3             r42hc72bb7e_0    conda-forge
r-dplyr                   1.1.4             r42ha503ecb_0    conda-forge
r-ellipsis                0.3.2             r42h57805ef_2    conda-forge
r-fansi                   1.0.6             r42h57805ef_0    conda-forge
r-farver                  2.1.2             r42ha18555a_0    conda-forge
r-generics                0.1.3             r42hc72bb7e_2    conda-forge
r-ggplot2                 3.5.1             r42hc72bb7e_0    conda-forge
r-glue                    1.7.0             r42h57805ef_0    conda-forge
r-gtable                  0.3.5             r42hc72bb7e_0    conda-forge
r-here                    1.0.1             r42hc72bb7e_2    conda-forge
r-isoband                 0.2.7             r42ha503ecb_2    conda-forge
r-labeling                0.4.3             r42hc72bb7e_0    conda-forge
r-lattice                 0.22_6            r42h57805ef_0    conda-forge
r-lifecycle               1.0.4             r42hc72bb7e_0    conda-forge
r-magrittr                2.0.3             r42h57805ef_2    conda-forge
r-mass                    7.3_60.0.1        r42h57805ef_0    conda-forge
r-matrix                  1.6_5             r42h316c678_0    conda-forge
r-mgcv                    1.9_1             r42h316c678_0    conda-forge
r-munsell                 0.5.1             r42hc72bb7e_0    conda-forge
r-nlme                    3.1_165           r42hbcb9c34_0    conda-forge
r-pillar                  1.9.0             r42hc72bb7e_1    conda-forge
r-pkgconfig               2.0.3             r42hc72bb7e_3    conda-forge
r-purrr                   1.0.2             r42h57805ef_0    conda-forge
r-r6                      2.5.1             r42hc72bb7e_2    conda-forge
r-rcolorbrewer            1.1_3             r42h785f33e_2    conda-forge
r-rlang                   1.1.4             r42ha18555a_0    conda-forge
r-rprojroot               2.0.4             r42hc72bb7e_0    conda-forge
r-scales                  1.3.0             r42hc72bb7e_0    conda-forge
r-stringi                 1.7.12            r42h1ae9187_0    conda-forge
r-stringr                 1.5.1             r42h785f33e_0    conda-forge
r-tibble                  3.2.1             r42h57805ef_2    conda-forge
r-tidyr                   1.3.1             r42ha503ecb_0    conda-forge
r-tidyselect              1.2.1             r42hc72bb7e_0    conda-forge
r-utf8                    1.2.4             r42h57805ef_0    conda-forge
r-vctrs                   0.6.5             r42ha503ecb_0    conda-forge
r-viridislite             0.4.2             r42hc72bb7e_1    conda-forge
r-withr                   3.0.0             r42hc72bb7e_0    conda-forge
re2                       2023.02.01           hcb278e6_0    conda-forge
readline                  8.2                  h8228510_1    conda-forge
recon                     1.08                 h031d066_7    bioconda
regex                     2024.7.24       py310h5b4e0ec_0    conda-forge
repeatmasker              4.1.2.p1        pl5321hdfd78af_1    bioconda
repeatmodeler             2.0.3           pl5321h9ee0642_0    bioconda
repeatscout               1.0.6                hec16e2b_3    bioconda
requests                  2.32.3             pyhd8ed1ab_0    conda-forge
requests-oauthlib         2.0.0              pyhd8ed1ab_0    conda-forge
rmblast                   2.14.1               h4565617_0    bioconda
rpsbproc                  0.5.0                h6a68c12_0    bioconda
rsa                       4.9                pyhd8ed1ab_0    conda-forge
s2n                       1.3.37               h3358134_0    conda-forge
scikit-learn              1.5.1           py310h146d792_0    conda-forge
scipy                     1.14.1          py310ha3fb0e1_0    conda-forge
sed                       4.8                  he412f7d_0    conda-forge
seqtk                     1.4                  he4a0461_2    bioconda
setuptools                72.2.0             pyhd8ed1ab_0    conda-forge
six                       1.16.0             pyh6c4a22f_0    conda-forge
snappy                    1.1.10               hdb0a2a9_1    conda-forge
sortedcontainers          2.4.0              pyhd8ed1ab_0    conda-forge
swifter                   1.4.0              pyhd8ed1ab_0    conda-forge
sysroot_linux-64          2.17                h4a8ded7_16    conda-forge
tblib                     3.0.0              pyhd8ed1ab_0    conda-forge
tensorboard               2.11.2             pyhd8ed1ab_0    conda-forge
tensorboard-data-server   0.6.1           py310h600f1e7_4    conda-forge
tensorboard-plugin-wit    1.8.1              pyhd8ed1ab_0    conda-forge
tensorflow                2.11.0          cpu_py310hd1aba9c_0    conda-forge
tensorflow-base           2.11.0          cpu_py310hc9b7e7f_0    conda-forge
tensorflow-estimator      2.11.0          cpu_py310hfed9998_0    conda-forge
termcolor                 2.4.0              pyhd8ed1ab_0    conda-forge
tesorter                  1.4.6              pyhdfd78af_1    bioconda
threadpoolctl             3.5.0              pyhc1e730c_0    conda-forge
tk                        8.6.13          noxft_h4845f30_101    conda-forge
tktable                   2.10                 h8bc8fbc_6    conda-forge
toolz                     0.12.1             pyhd8ed1ab_0    conda-forge
tornado                   6.4.1           py310hc51659f_0    conda-forge
tqdm                      4.66.5             pyhd8ed1ab_0    conda-forge
trf                       4.09.1               h031d066_5    bioconda
typing-extensions         4.12.2               hd8ed1ab_0    conda-forge
typing_extensions         4.12.2             pyha770c72_0    conda-forge
tzdata                    2024a                h0c530f3_0    conda-forge
ucsc-fatotwobit           455                  h2a80c09_1    bioconda
ucsc-twobitinfo           455                  h2a80c09_1    bioconda
ucsc-twobittofa           455                  h2a80c09_1    bioconda
unicodedata2              15.1.0          py310h2372a71_0    conda-forge
urllib3                   2.2.2              pyhd8ed1ab_1    conda-forge
werkzeug                  3.0.4              pyhd8ed1ab_0    conda-forge
wget                      1.21.4               hda4d442_0    conda-forge
wheel                     0.44.0             pyhd8ed1ab_0    conda-forge
wrapt                     1.16.0          py310h2372a71_0    conda-forge
xopen                     2.0.2              pyh707e725_1    conda-forge
xorg-fixesproto           5.0               h7f98852_1002    conda-forge
xorg-inputproto           2.3.2             h7f98852_1002    conda-forge
xorg-kbproto              1.0.7             h7f98852_1002    conda-forge
xorg-libice               1.0.10               h7f98852_0    conda-forge
xorg-libsm                1.2.3             hd9c2040_1000    conda-forge
xorg-libx11               1.8.4                h0b41bf4_0    conda-forge
xorg-libxau               1.0.11               hd590300_0    conda-forge
xorg-libxdmcp             1.1.3                h7f98852_0    conda-forge
xorg-libxext              1.3.4                h0b41bf4_2    conda-forge
xorg-libxfixes            5.0.3             h7f98852_1004    conda-forge
xorg-libxi                1.7.10               h7f98852_0    conda-forge
xorg-libxrender           0.9.10            h7f98852_1003    conda-forge
xorg-libxt                1.3.0                hd590300_0    conda-forge
xorg-libxtst              1.2.3             h7f98852_1002    conda-forge
xorg-recordproto          1.14.2            h7f98852_1002    conda-forge
xorg-renderproto          0.11.1            h7f98852_1002    conda-forge
xorg-xextproto            7.3.0             h0b41bf4_1003    conda-forge
xorg-xproto               7.0.31            h7f98852_1007    conda-forge
xyzservices               2024.6.0           pyhd8ed1ab_0    conda-forge
xz                        5.2.6                h166bdaf_0    conda-forge
yaml                      0.2.5                h7f98852_2    conda-forge
yarl                      1.9.4           py310h2372a71_0    conda-forge
zict                      3.0.0              pyhd8ed1ab_0    conda-forge
zipp                      3.20.0             pyhd8ed1ab_0    conda-forge
zlib                      1.2.13               h4ab18f5_6    conda-forge
zlib-ng                   2.2.1                he02047a_0    conda-forge
zstandard                 0.23.0          py310h64cae3c_0    conda-forge
zstd                      1.5.6                ha6fb4c9_0    conda-forge

@zyy36920
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zyy36920 commented Sep 9, 2024 via email

@zyy36920
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zyy36920 commented Sep 9, 2024 via email

@oushujun
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oushujun commented Oct 7, 2024

Hello,

The conda recipe has not yet been updated, so please use the following command to install dependencies:
For EDTA:
mamba create -n EDTA -c conda-forge -c bioconda -c r annosine2 biopython blast cd-hit coreutils genericrepeatfinder genometools-genometools glob2 h5py==3.9 keras==2.11 ltr_finder ltr_retriever mdust multiprocess muscle openjdk pandas perl perl-text-soundex pyarrow python r-base r-dplyr regex repeatmodeler r-ggplot2 r-here r-tidyr scikit-learn swifter tensorflow==2.11 tesorter

For EDTA2
mamba create -n EDTA2.2 -c conda-forge -c bioconda -c r annosine2 biopython cd-hit coreutils genericrepeatfinder genometools-genometools glob2 tir-learner ltr_finder_parallel ltr_retriever mdust multiprocess muscle openjdk perl perl-text-soundex r-base r-dplyr regex repeatmodeler r-ggplot2 r-here r-tidyr tesorter samtools bedtools LTR_HARVEST_parallel HelitronScanner
To use EDTA2, you need to switch to the EDTA2 branch on your local version:
cd ~/path-to-EDTA/EDTA
git checkout EDTA2
git branch

Thanks!
Shujun

@jwli-code
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Hello,

The conda recipe has not yet been updated, so please use the following command to install dependencies: For EDTA: mamba create -n EDTA -c conda-forge -c bioconda -c r annosine2 biopython blast cd-hit coreutils genericrepeatfinder genometools-genometools glob2 h5py==3.9 keras==2.11 ltr_finder ltr_retriever mdust multiprocess muscle openjdk pandas perl perl-text-soundex pyarrow python r-base r-dplyr regex repeatmodeler r-ggplot2 r-here r-tidyr scikit-learn swifter tensorflow==2.11 tesorter

For EDTA2 mamba create -n EDTA2.2 -c conda-forge -c bioconda -c r annosine2 biopython cd-hit coreutils genericrepeatfinder genometools-genometools glob2 tir-learner ltr_finder_parallel ltr_retriever mdust multiprocess muscle openjdk perl perl-text-soundex r-base r-dplyr regex repeatmodeler r-ggplot2 r-here r-tidyr tesorter samtools bedtools LTR_HARVEST_parallel HelitronScanner To use EDTA2, you need to switch to the EDTA2 branch on your local version: cd ~/path-to-EDTA/EDTA git checkout EDTA2 git branch

Thanks! Shujun

The issue of multiple warnings during test data execution. I'm not sure if these warning messages will affect the results. I followed the installation commands and ran the test command.

perl ../EDTA.pl --genome genome.fa --cds genome.cds.fa --curatedlib ../database/rice7.0.0.liban --exclude genome.exclude.bed --overwrite 1 --sensitive 1 --anno 1 --threads 10

This is my log.

cat EDTA_test.out

#########################################################
##### Extensive de-novo TE Annotator (EDTA) v2.2.2  #####
##### Shujun Ou (shujun.ou.1@gmail.com)             #####
#########################################################


Parameters: --genome genome.fa --cds genome.cds.fa --curatedlib ../database/rice7.0.0.liban --exclude genome.exclude.bed --overwrite 1 --sensitive 1 --anno 1 --threads 10


Fri Nov  1 20:12:57 CST 2024    Dependency checking:
        All passed!

        A custom library ../database/rice7.0.0.liban is provided via --curatedlib. Please make sure this is a manually curated library but not machine generated.

        A CDS file genome.cds.fa is provided via --cds. Please make sure this is the DNA sequence of coding regions only.

        A BED file is provided via --exclude. Regions specified by this file will be excluded from TE annotation and masking.

Fri Nov  1 20:12:59 CST 2024    Obtain raw TE libraries using various structure-based programs:
Fri Nov  1 20:16:20 CST 2024    Obtain raw TE libraries finished.
        All intact TEs found by EDTA:
                genome.fa.mod.EDTA.intact.raw.fa
                genome.fa.mod.EDTA.intact.raw.gff3

Fri Nov  1 20:16:20 CST 2024    Perform EDTA advance filtering for raw TE candidates and generate the stage 1 library:

Fri Nov  1 20:16:34 CST 2024    EDTA advance filtering finished.

Fri Nov  1 20:16:34 CST 2024    Perform EDTA final steps to generate a non-redundant comprehensive TE library.

        Filter RepeatModeler results that are ignored in the raw step.

Fri Nov  1 20:16:38 CST 2024    Clean up TE-related sequences in the CDS file with TEsorter.

        Remove CDS-related sequences in the EDTA library.

        Remove CDS-related sequences in intact TEs.

Fri Nov  1 20:16:45 CST 2024    Combine the high-quality TE library rice7.0.0.liban with the EDTA library:

Fri Nov  1 20:16:52 CST 2024    EDTA final stage finished! You may check out:
                The final EDTA TE library: genome.fa.mod.EDTA.TElib.fa
                Family names of intact TEs have been updated by rice7.0.0.liban: genome.fa.mod.EDTA.intact.gff3
        Comparing to the provided library, EDTA found these novel TEs: genome.fa.mod.EDTA.TElib.novel.fa
                The provided library has been incorporated into the final library: genome.fa.mod.EDTA.TElib.fa

Fri Nov  1 20:16:52 CST 2024    Perform post-EDTA analysis for whole-genome annotation:

Fri Nov  1 20:17:02 CST 2024    TE annotation using the EDTA library has finished! Check out:
                Whole-genome TE annotation (total TE: 34.61%): genome.fa.mod.EDTA.TEanno.gff3
                Whole-genome TE annotation summary: genome.fa.mod.EDTA.TEanno.sum
                Whole-genome TE divergence plot: genome.fa.mod_divergence_plot.pdf
                Whole-genome TE density plot: genome.fa.mod.EDTA.TEanno.density_plots.pdf
                Low-threshold TE masking for MAKER gene annotation (masked: 17.27%): genome.fa.mod.MAKER.masked

Fri Nov  1 20:17:02 CST 2024    Evaluate the level of inconsistency for whole-genome TE annotation:

Fri Nov  1 20:17:07 CST 2024    Evaluation of TE annotation finished! Check out these files:

                                Overall: genome.fa.mod.EDTA.TE.fa.stat.all.sum
                                Nested: genome.fa.mod.EDTA.TE.fa.stat.nested.sum
                                Non-nested: genome.fa.mod.EDTA.TE.fa.stat.redun.sum

                If you want to learn more about the formatting and information of these files, please visit:
                        https://github.com/oushujun/EDTA/wiki/Making-sense-of-EDTA-usage-and-outputs---Q&A

This is my errlog

Fri Nov  1 20:12:59 CST 2024    EDTA_raw: Check dependencies, prepare working directories.

Fri Nov  1 20:13:10 CST 2024    Start to find LTR candidates.

Fri Nov  1 20:13:11 CST 2024    Identify LTR retrotransposon candidates from scratch.

Warning: LOC list genome.fa.mod.ltrTE.veryfalse is empty.
Fri Nov  1 20:13:29 CST 2024    Finish finding LTR candidates.

Fri Nov  1 20:13:29 CST 2024    Start to find SINE candidates.

Fri Nov  1 20:13:56 CST 2024    Warning: The SINE result file has 0 bp!

Fri Nov  1 20:13:56 CST 2024    Start to find LINE candidates.

Fri Nov  1 20:13:56 CST 2024    Identify LINE retrotransposon candidates from scratch.

Fri Nov  1 20:15:15 CST 2024    Warning: The LINE result file has 0 bp!

Fri Nov  1 20:15:15 CST 2024    Start to find TIR candidates.

Fri Nov  1 20:15:15 CST 2024    Identify TIR candidates from scratch.

Species: others
Fri Nov  1 20:15:48 CST 2024    Finish finding TIR candidates.

Fri Nov  1 20:15:48 CST 2024    Start to find Helitron candidates.

Fri Nov  1 20:15:48 CST 2024    Identify Helitron candidates from scratch.

Fri Nov  1 20:16:20 CST 2024    Finish finding Helitron candidates.

Fri Nov  1 20:16:20 CST 2024    Execution of EDTA_raw.pl is finished!


Warning: No sequences were masked
Fri Nov  1 20:16:52 CST 2024    Homology-based annotation of TEs using genome.fa.mod.EDTA.TElib.fa from scratch.

@oushujun
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oushujun commented Nov 10, 2024 via email

@oushujun
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The new conda version (v2.2.2, https://anaconda.org/bioconda/edta) should be installed fine now. Unpinning this issue.

Shujun

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