Closed
Description
openedon Nov 12, 2019
Hi, I have a problem.
How can I solve the problem?
[0;35m[nf-core/hlatyping] Pipeline completed with errors
Error executing process > 'remap_to_hla (1)'
Caused by:
Process `remap_to_hla (1)` terminated with an error exit status (1)
Command executed:
samtools view -@ 1 -h -f 0x40 example_pe.bam > output_1.bam
samtools view -@ 1 -h -f 0x80 example_pe.bam > output_2.bam
samtools bam2fq output_1.bam > output_1.fastq
samtools bam2fq output_2.bam > output_2.fastq
yara_mapper -e 3 -t 1 -f bam /root/.nextflow/assets/nf-core/hlatyping/data/indices/yara/hla_reference_dna output_1.fastq output_2.fastq > output.bam
samtools view -@ 1 -h -F 4 -f 0x40 -b1 output.bam > mapped_1.bam
samtools view -@ 1 -h -F 4 -f 0x80 -b1 output.bam > mapped_2.bam
Command exit status:
1
Command output:
(empty)
Command error:
[M::bam2fq_mainloop] discarded 0 singletons
[M::bam2fq_mainloop] processed 44315 reads
[M::bam2fq_mainloop] discarded 0 singletons
[M::bam2fq_mainloop] processed 44315 reads
Open failed on file /root/.nextflow/assets/nf-core/hlatyping/data/indices/yara/hla_reference_dna.txt.size: "No such file or directory"
yara_mapper: Error while opening reference file.
Work dir:
/data1/k2/ramu_optitype_out/results/work/99/143a4235e86bfbb45a9027550bc866
Tip: you can replicate the issue by changing to the process work dir and entering the command bash .command.run
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