OpenPrimeRui suddenly dies

[alexlenail]

I'm trying to design 155 reverse primers for a multiplex PCR experiment using OpenPrimeRui, but running into errors with the docker container. The logs look like:

a) Filtering of primers
o primer_length: 24645 primers remaining (100%)
o gc_clamp: 11544 primers remaining (46.84%)
o gc_ratio: 7033 primers remaining (28.54%)
o no_runs: 6886 primers remaining (27.94%)
o no_repeats: 6886 primers remaining (27.94%)
o melting_temp_range: 5536 primers remaining (22.46%)

And then the container suddenly dies. Do you know how I might be able to discover the cause of the issue?

[matdoering]

Hi @alexlenail,
Seems like it's being stuck in the next constraint evaluation after the melting temp evaluation. If you want to find the cause of the issue, you can take the package source code and start with some printout sections, if you really want do dive deep into this. The compute.constraints function in constraints_eval.R may be a good start.

Good luck :-)