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update reference file path
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baigal628 committed Jun 20, 2021
1 parent cc45707 commit 60792ce
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Showing 2 changed files with 11 additions and 11 deletions.
18 changes: 9 additions & 9 deletions MAESTRO/MAESTRO_PipeInit.py
Original file line number Diff line number Diff line change
Expand Up @@ -68,13 +68,13 @@ def scatac_parser(subparsers):
required = True,
help = "Path of the giggle annotation file required for regulator identification. "
"Please download the annotation file from "
"http://cistrome.org/~chenfei/MAESTRO/giggle.tar.gz and decompress it.")
"http://cistrome.org/~galib/MAESTRO/references/giggle.all.tar.gz and decompress it.")
group_reference.add_argument("--fasta", dest = "fasta", type = str,
default = "",
help = "Genome fasta file for mapping."
"Users can just download the fasta file for huamn and mouse from "
"http://cistrome.org/~galib/Refdata_scATAC_MAESTRO_GRCh38_1.1.0.tar.gz and "
"http://cistrome.org/~galib/Refdata_scATAC_MAESTRO_GRCm38_1.1.0.tar.gz, respectively and decompress them. "
"http://cistrome.org/~galib/MAESTRO/references/scATAC/Refdata_scATAC_MAESTRO_GRCh38_1.1.0.tar.gz and "
"http://cistrome.org/~galib/MAESTRO/references/scATAC/Refdata_scATAC_MAESTRO_GRCm38_1.1.0.tar.gz, respectively and decompress them. "
"For example, 'Refdata_scATAC_MAESTRO_GRCh38_1.1.0/GRCh38_genome.fa'.")
group_reference.add_argument("--index", dest = "index", type = str,
default = "",
Expand Down Expand Up @@ -228,14 +228,14 @@ def scrna_parser(subparsers):
group_reference.add_argument("--mapindex", dest = "mapindex",
required = True,
help = "Genome index directory for STAR. Users can just download the index file for human and mouse "
"from http://cistrome.org/~galib/Refdata_scRNA_MAESTRO_GRCh38_1.2.2.tar.gz and "
"http://cistrome.org/~galib/Refdata_scRNA_MAESTRO_GRCm38_1.2.2.tar.gz, respectively, and decompress them. "
"from http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCh38_1.2.2.tar.gz and "
"http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCm38_1.2.2.tar.gz, respectively, and decompress them. "
"Then specify the index directory for STAR, for example, 'Refdata_scRNA_MAESTRO_GRCh38_1.2.2/GRCh38_STAR_2.7.6a'.")
group_reference.add_argument("--rsem", dest = "rsem", default = "",
help = "The prefix of transcript references for RSEM used by rsem-prepare-reference (Only required when the platform is Smartseq2). "
"Users can directly download the annotation file for huamn and mouse from "
"http://cistrome.org/~chenfei/MAESTRO/Refdata_scRNA_MAESTRO_GRCh38_1.1.0.tar.gz and "
"http://cistrome.org/~chenfei/MAESTRO/Refdata_scRNA_MAESTRO_GRCm38_1.1.0.tar.gz, respectively, and decompress them. "
"http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCh38_1.1.0.tar.gz and "
"http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCm38_1.1.0.tar.gz,, respectively, and decompress them. "
"Then specify the prefix for RSEM, for example, 'Refdata_scRNA_MAESTRO_GRCh38_1.1.0/GRCh38_RSEM_1.3.2/GRCh38'.")

# Barcode arguments
Expand Down Expand Up @@ -266,8 +266,8 @@ def scrna_parser(subparsers):
# Regulator identification
group_regulator = workflow.add_argument_group("Regulator identification arguments")
group_regulator.add_argument("--lisadir", dest = "lisadir", type = str, default = "",
help = "Path to lisa data files. For human and mouse, data can be downloaded from http://cistrome.org/~alynch/data/lisa_data/hg38_2.1.tar.gz"
"and http://cistrome.org/~alynch/data/lisa_data/mm10_2.1.tar.gz")
help = "Path to lisa data files. For human and mouse, data can be downloaded from http://cistrome.org/~alynch/data/lisa_data/hg38_1000_2.0.h5"
"and http://cistrome.org/~alynch/data/lisa_data/mm10_1000_2.0.h5")


# Signature file arguments
Expand Down
4 changes: 2 additions & 2 deletions MAESTRO/Snakemake/scRNA/config_template.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -53,12 +53,12 @@ cutoff:
# Reference genome
genome:
# Genome index directory for STAR. Users can just download the index file
# from http://cistrome.org/~chenfei/MAESTRO/Refdata_scRNA_MAESTRO_GRCh38_1.1.0.tar.gz and decompress it.
# from http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCh38_1.2.2.tar.gz and decompress it.
# Then specify the index directory for STAR, for example, 'Refdata_scRNA_MAESTRO_GRCh38_1.1.0/GRCh38_STAR_2.7.3a'.
mapindex: {{ mapindex }}
# The prefix of transcript references for RSEM used by rsem-prepare-reference (Only required when the platform is Smartseq2).
# Users can directly download the annotation file from
# http://cistrome.org/~chenfei/MAESTRO/giggle.tar.gz and decompress it.
# http://cistrome.org/~galib/MAESTRO/references/scRNA/Refdata_scRNA_MAESTRO_GRCh38_1.1.0.tar.gz and decompress it.
# Then specify the prefix for RSEM, for example, 'Refdata_scRNA_MAESTRO_GRCh38_1.1.0/GRCh38_RSEM_1.3.2/GRCh38'.
rsem: {{ rsem }}

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