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alexandriai168 committed Aug 18, 2023
2 parents 5394241 + 886808d commit a68e38e
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6 changes: 3 additions & 3 deletions README.md
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Expand Up @@ -47,19 +47,19 @@ cp ./bin/* ~/Desktop/muri_metabarcoding/scripts
cp -r ./metadata/* ~/Desktop/muri_metabarcoding/metadata
```

## Step 3: Move Raw Fastq's into raw_fastq
## Step 3: Move Raw Fastq's into raw_fastq and Sample Sheet to metadata
---
Copy your raw fastq's into the raw_fastq directory. A command that would do this would look like:

```
cp /path/to/fastqs/* /path/to/raw_fastqs
cp /path/to/fastqs/* /path/to/muri_metabarcoding/raw_fastqs
```
Then, copy your Miseq Sample Sheet (which is produced after Illumina sequencing) to the metadata file.
```
cp /path/to/SampleSheetUsed.csv /path/to/muri_metabarcoding/metadata
```

## Step 4: Run metabarcoding_wrapper.sh
## Step 4: Run metabarcoding_wrapper.sh
---
The metabarcoding wrapper takes 2 inputs:
* path to your file system (named muri_metabarcoding)
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