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help updated GG2
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Falk Hildebrand (QIB) committed Sep 5, 2023
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2 changes: 1 addition & 1 deletion lotus2
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Expand Up @@ -3331,7 +3331,7 @@ my %workflow_options = (

my $taxonomy_heading = "Taxonomy Options";
my %taxonomy_options = (
'-refDB <SLV|GG|HITdb|PR2|UNITE|beetax>', '(SLV) Silva LSU (23/28S) or SSU (16/18S), (GG greengenes (only SSU available), (HITdb) (SSU, human gut specific), (PR2) LSU spezialized on Ocean environmentas, (UNITE) ITS fungi specific, (beetax) bee gut specific database and tax names. Given that \"-amplicon_type \" was set to SSU or LSU, the appropriate DB in SLV would be used. \nDecide which reference DB will be used for a similarity based taxonomy annotation. Databases can be combined, with the first having the highest priority. E.g. "HITdb,SLV" would priority assign OTUs to PR2 taxonomy, but hits with a higher %id to SLV would be assigned to SLV. Can also be a custom fasta formatted database: in this case provide the path to the fasta file as well as the path to the taxonomy for the sequences using -tax4refDB. For custom databases QIIME2 file formats are compatible if the delimiter in the QIIME2 taxonomy file is changed from semicolon to tab. See also online help on how to create a custom DB. WARNING: combining databases with incompatible tax levels (e.g. PR2,SLV) will result in non sensical tax levels. (Default: SLV)',
'-refDB <SLV|GG2|HITdb|PR2|UNITE|beetax>', '(SLV) Silva LSU (23/28S) or SSU (16/18S), (GG2) GreenGenes2 (only SSU available), (HITdb) (SSU, human gut specific), (PR2) LSU spezialized on Ocean environmentas, (UNITE) ITS fungi specific, (beetax) bee gut specific database and tax names. Given that \"-amplicon_type \" was set to SSU or LSU, the appropriate DB in SLV would be used. \nDecide which reference DB will be used for a similarity based taxonomy annotation. Databases can be combined, with the first having the highest priority. E.g. "HITdb,SLV" would priority assign OTUs to PR2 taxonomy, but hits with a higher %id to SLV would be assigned to SLV. Can also be a custom fasta formatted database: in this case provide the path to the fasta file as well as the path to the taxonomy for the sequences using -tax4refDB. For custom databases QIIME2 file formats are compatible if the delimiter in the QIIME2 taxonomy file is changed from semicolon to tab. See also online help on how to create a custom DB. WARNING: combining databases with incompatible tax levels (e.g. PR2,SLV) will result in non sensical tax levels. (Default: SLV)',
'-tax4refDB <file>', 'In conjunction with a custom fasta file provided to argument -refDB, this file contains for each fasta entry in the reference DB a taxonomic annotation string, with the same number of taxonomic levels for each, tab separated.',
'-amplicon_type <SSU|LSU|ITS|ITS1|ITS2|custom>', '(SSU) small subunit (16S/18S), (LSU) large subunit (23S/28S) or internal transcribed spacer (ITS|ITS1|ITS2), (custom) for custom marker genes. These options will change default read qual filter parameters and activate ITS specific postfiltering steps. (Default: SSU)',
'-tax_group <bacteria|fungi|eukarya>', '(bacteria) bacterial 16S rDNA annnotation, (fungi) fungal 18S/23S/ITS annotation, (eukarya) eukaryotic (18S/23S) annotation. (Default: bacteria)',
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