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Single-cell RNA-seq analysis pipeline


Pipeline goal:

Perform single-cell RNA-seq analysis from FastQ files to cerebro file for 10XGenomics technology data.


Available Steps:

  • Alignment_countTable_GE,
  • Alignment_countTable_ADT,
  • Alignment_annotations_TCR_BCR,
  • Droplets_QC_GE,
  • Filtering_GE,
  • Norm_DimRed_Eval_GE,
  • Clust_Markers_Annot_GE,
  • Adding_ADT,
  • Adding_TCR,
  • Adding_BCR,
  • Cerebro.


Future Developments:

  • Integration analysis of several samples,
  • Grouped analysis (no integration) of several samples,
  • TCR for several samples together,
  • BCR for several samples together,
  • Improve cell annotation,
  • ...

See complete documentation on the wiki