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IGESWorkshop2023 License: MIT

Analysis of single-cell RNA sequencing data

Abstract

Single-cell technologies are transforming our understanding of complex biological systems and enabling key insights into molecular mechanisms of disease. Recent years have witnessed the rapid accumulation of single-cell RNA sequencing (scRNA-seq) datasets in a wide range of applications. This workshop will provide a practical introduction to the analysis of scRNA-seq data and the diversity of existing computational tools. Topics will include quality control, normalization, dimensionality reduction, clustering, RNA velocity, and differential expression analysis. Finally, we will explore integrative approaches linking scRNA-seq and genome-wide association studies (GWAS) data to investigate disease mechanisms.

Workshop participants will have a working knowledge of R and must have RStudio and the Seurat library installed.

Educational Workshop Presentations

Dr. Eric R. Gamazon is broadly interested in genomics, computational biology, machine learning, and genetic medicine. He is an Associate Professor in the Division of Genetic Medicine and the Vanderbilt Genetics Institute and leads an interdisciplinary laboratory of computational scientists and molecular biologists. An ongoing project involves understanding gene regulation across tissues and cell types to gain insights into disease mechanisms and therapeutic targets. He served as a co-chair of the GWAS Working Group of the Genotype-Tissue Expression (GTEx) project. He was a recipient of a Genomic Innovator Award (2019) from the National Institutes of Health and is a Life Member of Clare Hall, University of Cambridge.

Hanna Abe is a PhD student in the Human Genetics program at Vanderbilt University. She is completing her graduate thesis on genetic regulation of gene expression across DNA variants and functional contexts. Her research interests lie in the fields of single-cell biology, computational biology, and statistical genetics. Her work involves the development of cell-type specific genetic models to characterize the dynamics of gene expression regulation.

Symphony I, Loews Hotel
November 5, 2023
3:00p - 4:30p
https://site.pheedloop.com/event/IGES2023/speakers/SPEJJBXGBLI

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