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Merge pull request /issues/218 from jsarro13/patch-1
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Update 03-trimming.md
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valgartner authored Oct 20, 2021
2 parents e364206 + 01f17c0 commit 515e560
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9 changes: 7 additions & 2 deletions episodes/03-trimming.md
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Expand Up @@ -135,13 +135,18 @@ $ cp ~/.miniconda3/pkgs/trimmomatic-0.38-0/share/trimmomatic-0.38-0/adapters/Nex
We will also use a sliding window of size 4 that will remove bases if their
phred score is below 20 (like in our example above). We will also
discard any reads that do not have at least 25 bases remaining after
this trimming step. This command will take a few minutes to run.
this trimming step. Three additional pieces of code are also added to the end
of the ILLUMINACLIP step. These three additional numbers (2:40:15) tell
Trimmimatic how to handle sequence matches to the Nextera adapters. A detailed
explanation of how they work is advanced for this particular lesson. For now we
will use these numbers as a default and recognize they are needed to for Trimmomatic
to run properly. This command will take a few minutes to run.

~~~
$ trimmomatic PE SRR2589044_1.fastq.gz SRR2589044_2.fastq.gz \
SRR2589044_1.trim.fastq.gz SRR2589044_1un.trim.fastq.gz \
SRR2589044_2.trim.fastq.gz SRR2589044_2un.trim.fastq.gz \
SLIDINGWINDOW:4:20 MINLEN:25 ILLUMINACLIP:NexteraPE-PE.fa:2:40:15
SLIDINGWINDOW:4:20 MINLEN:25 ILLUMINACLIP:NexteraPE-PE.fa:2:40:15
~~~
{: .bash}

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