05 FastQC untrimmed RNA
FastQC is a quality control tool that gives information about NGS data. The result from FastQC can be viewed in more detail in analyses/05_pre_trim_FastQC_transcript. The trimming was done on the sample SRR6040095 which is from the aril of the durian. The per base sequence quality was not as good as it were when then DNA was analysed using FastQC, this is expected since these reads has not been trimmed yet so that bases with bad quality can still be present. The forward read scored quite bad but the reverse read scored quite good for being an untrimmed read.
Some of the questions that apply for FastQC in general are answered in the page 02 FastQC DNA
The quality is quite poor but this is since this is raw reads that has not been trimmed.
What can generate the issues you observe in your data? Can these cause any problems during subsequent analyses?
What generates issues is that some bases with low quality score is present and that some adapters that are left from the illumina sequencing still can be present.