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HDL-X

This repository contains all R Markdown files used for the data analysis presented in Cardner et al. (2020), doi: 10.1172/jci.insight.131491.

Main document structure

For reasons of data privacy, this repository contains no actual data, but merely the code used to analyse it. The raw data was collated and stored as tidy data in RDS files under computations/ (not committed). In general, the data frames were stored in a long format, with a column named key coding for the feature (clinical, protein, lipid) and a column named value recording the measurement. A column named Proband ID contained an anonymised subject code.

  • Normalisation
    • Proteomics and lipidomics in OmicsNormalisation.Rmd
    • NMR spectroscopy in Nightingale.Rmd
    • Functional bioassays in FunctionalNormalisation.Rmd
    • Clinical imputation in ClinicalImputation.Rmd
  • Mixed-effects modelling in MixedEffects.Rmd
  • Logistic regression in glmnet.Rmd
  • PLS regression in PLS.Rmd
  • Graphical lasso in HugeNPN.Rmd
  • Helper functions
    • helper_functions.R
    • helper_normalisation.R
    • helper_volcanoes.R

Additional analyses

In addition to the main documents, there are auxiliary R Markdown files for various purposes. Importantly, the JointGGM.Rmd explores the joint graphical lasso.

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