This virtual course will take place from 12:00 to 2:00 pm (Eastern Time) over five nonconsecutive days:
- Wednesday, December 2 (Agenda) ✅
- Friday, December 4 (Agenda) ✅
- Monday, December 7
- Wednesday, December 9
- Friday, December 11
There will be an optional pre-event tutoring session on Tuesday, December 1 from 12:00 - 2:00 pm
- Anne Deslattes Mays, Ph.D., Science and Technology Consulting LLC
- Christina Chatzipantsiou, Lifebit
The practice of biological inquiry has evolved so that a biological researcher's training must include both wet-lab and dry lab experimental and computational approaches. This course seeks to lay a broad foundation for the dry bench researcher. It is clear that "data literacy is a key skill in the modern world." This course will introduce the trainee to the computational techniques that enable data analysis locally on their laptop and as well as how to enable these same analysis techniques in the cloud.
In this workshop, we will also introduce the learner to the concept of team science. The learner will obtain critical skills in effective collaboration that is imperative in a world where the amount of data available is beyond a single lab's walls.
Over 10 hours of instruction, spread over two weeks, we seek to introduce the learner to RNAseq analysis and demystify concepts in RNAseq analysis. By the end of the course, the learner can successfully use tools involved in these analyses collaboratively and independently. This course aims to go broad and point the learner to learn more and take their knowledge deeper. Through their participation in the class, learners will acquire experience in a full suite of tools essential to any bioinformatics analysis. They will learn and understand FAIR best practices. They will obtain and use their GitHub, Docker, and ORCID ids. They will know where to find Nextflow workflows and run them to generate results that are further explored through interactive Jupyter Lab notebooks. They will learn about publicly available datasets downloadable from cloud resources and the ever-growing datasets that may aid their research. They will learn about using flexible, powerful, and community-based workflow languages such as Nextflow and how the resulting workflows use containerization to modularize and simplify bioinformatic analysis. Such workflows will be run using cloud-accessible data in the cloud and on their laptop. The course's final day will focus on pulling together all these resources into a single end-to-end example to empower the trainee to apply new knowledge towards their specific use case.
Pre-course materials as well as in-class exercises will cover the following topic areas:
- Bash
- R
- Git/GitHub
- Jupyter Notebooks
- Conda
- Docker
- Nextflow
Learners will use Lifebit's CloudOS platform for this course. Requirements include the availability of a laptop, as they will then have the ability to log into a browser to access the course materials (Chrome preferred).