ZoonoticMap is a cutting-edge computational tool designed to systematically analyze genomic data, enabling researchers to explore the zoonotic potential, antibiotic resistance, and pathogenicity of microbial genomes. It focuses on identifying and categorizing genes associated with pathogenicity, resistance, and mobile genetic elements like Integrative Conjugative Elements (ICE). This versatile tool facilitates integrative studies, offering valuable insights for microbial research and public health.
Gene Annotation and Classification: Automatically annotates genomic data with predefined categories such as Resistance Genes, Efflux Genes, T3SS, T4SS, and more. Pathogenicity Scoring: Computes a total score based on gene categories to classify genomes into High, Moderate, or Low Risk of pathogenicity. Customizable Weights: Assigns predefined weights to different gene categories for accurate risk assessment. Blast Integration: Utilizes BLAST for high-accuracy alignment of query genomes against a reference database. User-Friendly Output: Outputs a comprehensive Excel file with detailed results, including gene counts, risk classification, and annotations.
Zoonotic potential mapping for microbial genomes. Antibiotic resistance profiling. Pathogenicity prediction and risk assessment. Integrative studies on mobile genetic elements.
Input a query genome and a reference database of annotated genes. ZoonoticMap performs BLAST alignment to identify matching genes with high accuracy. Generates an annotated results file with categorized gene counts, total scores, and pathogenicity risk levels.
Python 3.x BLAST+ toolkit Python libraries: Biopython, Pandas, OpenPyxl
bash
Copy code Clone the repository
git clone --depth=1 https://github.com/Umeshkumarku1/ZoonoticMap.git
Navigate to the directory cd ZoonoticMap
Install required dependencies pip install -r requirements.txt
Run the analysis python zoonoticmap.py
Contributions are welcome! If you'd like to report issues or suggest enhancements, you can just submit a pull request or open an issue.
ZoonoticMap aims to empower researchers and bioinformaticians with a reliable tool for genomic analysis and pathogen risk assessment. Start exploring the world of microbial genomics today! If you have further queries kindly contact @research.umeshkumarku@gmail.com