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update readme
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PengNi committed Jun 8, 2023
1 parent 72185d4 commit edf11bf
Showing 1 changed file with 4 additions and 6 deletions.
10 changes: 4 additions & 6 deletions README.md
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Expand Up @@ -113,14 +113,13 @@ ccsmeth call_hifi --subreads /path/to/subreads.bam \


# 2. call modifications
# outputs: [--output].per_readsite.tsv,
# [--output].modbam.bam
# output: [--output].modbam.bam
CUDA_VISIBLE_DEVICES=0 ccsmeth call_mods \
--input /path/to/output.hifi.bam \
--model_file /path/to/ccsmeth/models/model_call_mods.ckpt \
--output /path/to/output.hifi.call_mods \
--threads 10 --threads_call 2 --model_type attbigru2s \
--rm_per_readsite
--mode denovo


# 3. align hifi reads
Expand Down Expand Up @@ -173,15 +172,14 @@ ccsmeth align_hifi \


# 3. call modifications
# outputs: [--output].per_readsite.tsv,
# [--output].modbam.bam
# output: [--output].modbam.bam
CUDA_VISIBLE_DEVICES=0 ccsmeth call_mods \
--input /path/to/output.hifi.pbmm2.bam \
--ref /path/to/genome.fa \
--model_file /path/to/ccsmeth/models/model_call_mods.ckpt \
--output /path/to/output.hifi.pbmm2.call_mods \
--threads 10 --threads_call 2 --model_type attbigru2s \
--rm_per_readsite --mode align
--mode align


# 4. call modification frequency
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