Filament_Tools

Calculate the area-fraction of the image filled with (septin) filaments.

Getting started

To install the tool save the file filament_tools.ijm into the folder macros/toolsets of your FIJI installation.

Select the "filament tools" toolset from the >> button of the ImageJ launcher.

• press the a-button to analyze the active image
• press the b-button to analyze the images in a folder in batch-mode.

Options

Right-click the a-button to open the options dialog.

• filament channel - the number of the channel containing the filament image.
• min. area - the minimal area an object must have to be considered a filament.
• max. circularity - allows to remove objects too round to be filaments