Differential Co-Localization analysis (DiCoLo) is a computational framework designed for capturing condition-specific co-localized gene patterns within single-cell RNA sequencing data. The major workflow of DiCoLo comprises the following three main steps:
- Step1. Compute gene-gene OT distance for each condition
- Step2. Construct differential graph operator
- Step3. Identify differential co-localized genes by spectral analysis
- Downstream tasks
- Assemble significant DiCoLo genes into co-localized gene modules.
Please check the DiCoLo tutorial, available in both
Rmd
and
HTML.
The supporting functions used in the tutorial are available in
DiCoLo_functions.R.