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add page resource/genetics tools
Jun-Lizst · Aug 26, 2022 · a4a0132 · a4a0132
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diff --git a/content/page/about.md b/content/page/about.md
@@ -58,9 +58,9 @@ Major In Bioscience; Class of State Key Educational Base
 
 ## Research Experiences
 
-**Graduate student, Peking University & Chinese institute for Brain Research (CIBR)**\
+**Graduate student**, [Peking University](https://english.pku.edu.cn/) & [Chinese institute for Brain Research (CIBR)](https://www.cibr.ac.cn/)\
 MARCH 2019 — PRESENT\
-Graduate student advised by Dr.Wooping Ge, Ph.D. and Dr.Wenzhi Sun, Ph.D.
+Graduate student advised by [Dr.Wooping Ge, Ph.D.](https://www.cibr.ac.cn/science/team/detail/404?language=en) and [Dr.Wenzhi Sun, Ph.D.](https://www.cibr.ac.cn/science/team/detail/405?language=en)
 
 - Focusing on AAV capsid engineering and make molecular/genetic tools for study of brain 
 vascular system.
@@ -104,7 +104,7 @@ non-invasive manipulation of neuronal activity.
 **HZAU-iGEM team member,**
 Inovation Lab, College of Life science and technology, HZAU\
 FEBRUARY 2015 - OCTOBER 2016\
-2016 HZAU-iGEM team member with instructor Dr.BingGuang Ma, PhD
+2016 HZAU-iGEM team member with instructor [Dr.BingGuang Ma, PhD](http://encoi.hzau.edu.cn/info/1016/1034.htm)
 - Systems and Synthetic Biology learning: student presentation, case-based learning, and discussion
 - During the Competition and in our project, I am responsible for the Optical control part which uses light switchable circuit (LSC) to control the expression of bacteria movement related gene CheZ.
 - Website of HZAU-iGEM2016: http://2016.igem.org/Team:HZAU-China

diff --git a/content/resource/biochemistrytools.md b/content/resource/biochemistrytools.md
@@ -30,13 +30,21 @@ thumbnailImagePosition: left
 
 ### Ref Labs
 
+
+
 - **Signaling pathway and basic science**
 1. [Zhijian-James Chen lab, UTSW](https://www.james-zhijian-chen-lab.org/)
-
 2. [David M. Sabatini lab, MIT](https://www.davidmsabatini.com/)
-
 3. [Xiaodong Wang lab, UTSW, NIBS](http://www.nibs.ac.cn/en/yjsjyimgshow.php?cid=5&sid=6&id=779)
+4. [Feng Shao lab, NIBS](http://www.nibs.ac.cn/en/yjsjyimgshow.php?cid=5&sid=6&id=777)
+
+
 
 - **Tool development**
 1. [Alice Ting lab, Stanford](http://www.tinglab.org/)
 
+
+
+### Ref. Protocols
+1. [Bio-protocol](https://cn.bio-protocol.org/cn/default.aspx)
+2. [Protocol Exchange](https://protocolexchange.researchsquare.com/)
diff --git a/content/resource/geneticstools.md b/content/resource/geneticstools.md
@@ -0,0 +1,98 @@
+---
+title: "Genetics tools"
+date: 2022-08-26T16:50:25+08:00
+categories:
+- category
+- subcategory
+tags:
+- PEGASOS
+- CUBIC
+- Clarity
+keywords:
+- tech
+comments:       false
+showMeta:       false
+showActions:    false
+thumbnailImage: /img/refreshingyourstrains_blogpost.jpg
+thumbnailImagePosition: left
+#thumbnailImage: //example.com/image.jpg
+---
+
+---
+# Mus musculus
+![mus129s1](/img/mus_129s1_20220826.png) [129S1](https://www.jax.org/strain/002448)
+![c57bl6jx1](/img/b6j_20220826193408.png) [C57BL/6J](https://www.jax.org/strain/000664)
+![balbcjx1](/img/BALBcj_20220826194024.png) [BALB/cJ](https://www.jax.org/strain/000651)
+
+## Mouse genetics tools in brain vasculature system field
+1. **NG2DsRedBAC**: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. [[JAX Strain #:008241](https://www.jax.org/strain/008241) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=008241&protocolID=18794)] | [DOI Link](https://doi.org/10.1242/dev.004895)]
+2. **Tek-Cre**: The Tie2-Cre (Tek-Cre) transgene has the mouse endothelial-specific receptor tyrosine kinase (Tek or Tie2) promoter directing expression of Cre recombinase. These Tek-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in endothelial cells during embryogenesis and adulthood. **Cre recombinase activity is also detected in microglia in central nervous system.**  [[JAX Strain #:008863](https://www.jax.org/strain/008863) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=008863&protocolID=22392) | [DOI Link](https://doi.org/10.1006/dbio.2000.0106)]
+3. **Pdgfrb-cre**: Pericyte labeling. [DOI Link](https://doi.org/10.1002/dvg.20769)
+4. **Pdgfrb-CreERT2**: Pdgfrb-CreERT2 transgenic mice is suitable for Pericyte labeling, the result of Pdgfrb-CreERT2::Ai14 is very clean and specific. [[JAX Strain #:029684](https://www.jax.org/strain/029684) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=029684&protocolID=20745) | [DOI Link](https://doi.org/10.1038/ki.2015.274)]
+5. **BMX-CreERT2**: Artery EC labeling.  [Genotyping Protocol](https://static1.squarespace.com/static/542b27b6e4b04193388466d4/t/58ee4b7b9f745687d35f380f/1492011904519/Tg+%28BMX-CreERT2%29+Genotyping.pdf) 
+6. **Slco1c1-KI-P2A-iCreERT2**: Brain specific EC labeling(including spinal cord). [Woo-Ping Ge lab, Design by Jun-Liszt Li, [Contact](https://www.cibr.ac.cn/science/team/detail/404?language=en)]
+
+7. 
+
+
+
+
+#### Astrocyte labeling
+1. **Aldh1l1-EGFP**
+2. **Aldh1l1-CreERT2**
+3. **mGFAP-Cre**
+4. **hGFAP-Cre**: 
+5.
+
+
+
+
+
+#### Neuron labeling
+1. **Thy1-YFP-M**: These Thy1-GFP-M transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets. [[JAX Strain #:007788](https://www.jax.org/strain/007788) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=007788&protocolID=25728)]
+2. **Thy1-YFP-16J**: These thy1-YFP-16 transgenic mice express yellow fluorescent protein at high levels in motor and sensory neurons, as well as in subsets of central neurons. This line provides a strong and specific vital marker for axons, and expression is strong from a mid-gestational stage into adulthood. [[JAX Strain #:003709](https://www.jax.org/strain/003709) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=003709&protocolID=23870)]
+2. Gad1-EGFP: Also called Gad67-EGFP, Inhibitory GABAnergic neuron labeling. [[JAX Strain #:007677](https://www.jax.org/strain/007677) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=007677&protocolID=22102) | [DOI Link](https://doi.org/10.1523/jneurosci.1851-04.2004)] 
+
+3. Vgat-Cre: 
+4. Vip-Cre: Vip+ Interneuron labeling.
+5. Sst-Cre: Sst+ Interneuron labeling.
+6. Som-Cre: Som+ Interneuron labeling.
+7. Vglut1-Cre: Excitatory neuron labeling.
+8. 
+
+
+#### Microglia labeling
+1. **CX3CR1-EGFP**: [[JAX Strain #:005582](https://www.jax.org/strain/005582) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=005582&protocolID=27927)]
+2. CX3CR1-iCreERT2:
+3. **Tmem119-P2A-iCreERT2**:  [[JAX Strain #:031820](https://www.jax.org/strain/031820) | [Genotyping Protocol](https://www.jax.org/Protocol?stockNumber=031820&protocolID=34019) | [DOI Link](https://doi.org/10.1523/eneuro.0448-18.2019)]
+
+
+
+---
+### Ref. public websites
+1. [The Jackson Laborary](https://www.jax.org/): The laboratory is also the world's source for more than 8,000 strains of genetically defined mice, and serves as a worldwide center for scientific courses, conferences, training, and education.
+2. [Mouse Genome Informatics](http://www.informatics.jax.org/): MGI is the international database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data to facilitate the study of human health and disease.
+
+
+
+---
+### Ref Labs
+1. [Masashi Yanagisawa Lab](https://sleepymouse.jp/), The International Institute for Integrative Sleep(IIIS)
+2. [Z. Josh Huang Lab](https://www.neuro.duke.edu/research/faculty-labs/huang-lab), Duke University
+
+
+
+
+---
+# Rat
+
+
+
+---
+# Drosophila melanogaster
+
+
+---
+# Zebrafish
+
+
diff --git a/content/resource/molecularbiology.md b/content/resource/molecularbiology.md
@@ -51,28 +51,37 @@ Brain endothelial cell specific: AAV-BI30
 
 
 
+
 ## Tissue/Cell type specific promoter
 >
 ---
 
-Pan-neuron: hSyn
-Excitatory neuron: CaMKIIa
+- Pan-neuron: hSyn\
+Kügler, S., E. Kilic, and M. Bähr. "Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area." Gene therapy 10.4 (2003): 337-347. [[DOI Link](https://doi.org/10.1038/sj.gt.3301905) | [PDF]()]
+
+Excitatory neuron: CaMKIIa\
 Inhibitory neuron: pGAD1. mDlx
 
 
 Constitutive expression: EF1a, CAG
 
 Drug induced: Tet-on system
 
-activity depedent: c-Fos promoter
+Activity depedent: c-Fos promoter
+
+
+
 
 
 ## Genetically encoded indicators
-Calcium: GCaMP series (GCaMP8, GCaMP7, GCaMP6)\
-Potassium:
-Dopamine:
-Histamine:
-eCB:
+- Calcium: GCaMP series [[GCaMP8s/m/f](https://www.janelia.org/jgcamp8-calcium-indicators) |  [GCaMP7s/f/b/c](https://doi.org/10.1038/s41592-019-0435-6) |  [GCaMP6s/m/f](https://doi.org/10.1038/nature12354)]\
+- Potassium: [ ]\
+- Dopamine: GRAB-DA[[GRAB-DA1.0](https://doi.org/10.1016/j.cell.2018.06.042) | [GRAB-DA2.0](https://doi.org/10.1038/s41592-020-00981-9)]; dLight[[dLight1.2](https://doi.org/10.1126/science.aat4422), [addgene](https://www.addgene.org/111068/)]\
+- Histamine:
+- eCB:
+- Kinase activity sensor: PKA[]
+
+
 
 
 ## CRISPR systems

diff --git a/public/archives/index.html b/public/archives/index.html
@@ -304,6 +304,10 @@ <h4 class="archive-title" style="text-decoration: none;">2022</h4>
         <ul class="archive-posts archive-month" data-date="202208">
           <h5 class="archive-title" style="text-decoration: none;">August</h5>
 
+            <li class="archive-post archive-day" data-date="20220826">
+              <a class="archive-post-title" href="https://leoj.xyz/resource/geneticstools/">Genetics tools</a>
+            </li>
+
             <li class="archive-post archive-day" data-date="20220825">
               <a class="archive-post-title" href="https://leoj.xyz/resource/biochemistrytools/">Biochemistry tools</a>
             </li>

diff --git a/public/categories/category/index.html b/public/categories/category/index.html
@@ -306,6 +306,50 @@ <h5 class="sidebar-profile-bio">Hello, My name is Jun-Liszt Li. my science inter
 
 
 
+<article class="postShorten postShorten--thumbnailimg-left">
+  <div class="postShorten-wrap">
+
+    <div class="postShorten-header">
+      <h1 class="postShorten-title">
+
+        <a class="link-unstyled" href="https://leoj.xyz/resource/geneticstools/" aria-label="Open the post: Genetics tools">
+
+          Genetics tools
+        </a>
+      </h1>
+
+    </div>
+    <div class="postShorten-excerpt">
+
+        Mus musculus 129S1 C57BL/6J BALB/cJ
+Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS.
+
+      <p>
+
+          <a href="https://leoj.xyz/resource/geneticstools/" class="postShorten-excerpt_link link" aria-label="Open the post: Genetics tools">Continue reading</a>
+
+
+      </p>
+    </div>
+  </div>
+
+    <a href="https://leoj.xyz/resource/geneticstools/" aria-label="Open the post: Genetics tools">
+      <div class="postShorten-thumbnailimg">
+        <img alt="" src="https://leoj.xyz/img/refreshingyourstrains_blogpost.jpg"/>
+      </div>
+    </a>
+
+</article>
+
+
+
+
+
+
+
+
+
+
 <article class="postShorten postShorten--thumbnailimg-left">
   <div class="postShorten-wrap">
 
@@ -321,10 +365,7 @@ <h1 class="postShorten-title">
     </div>
     <div class="postShorten-excerpt">
 
-        Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW
-David M. Sabatini lab, MIT
-Xiaodong Wang lab, UTSW, NIBS
-Tool development Alice Ting lab, Stanford 
+        Vendor companies Abcam ThermoFisher R&amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange 
 
       <p>
 
@@ -374,11 +415,8 @@ <h1 class="postShorten-title">
 Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1
 Liver specific:
 Brain endothelial cell specific: AAV-BI30
-Tissue/Cell type specific promoter Pan-neuron: hSyn Excitatory neuron: CaMKIIa Inhibitory neuron: pGAD1. mDlx
-Constitutive expression: EF1a, CAG
-Drug induced: Tet-on system
-activity depedent: c-Fos promoter
-Genetically encoded indicators Calcium: GCaMP series (GCaMP8, GCaMP7, GCaMP6)
+Tissue/Cell type specific promoter Pan-neuron: hSyn
+Kügler, S., E. Kilic, and M. Bähr. &ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area.
 
       <p>
 

diff --git a/public/categories/category/index.xml b/public/categories/category/index.xml
@@ -6,17 +6,24 @@
     <description>Recent content in category on Jun-Liszt Li</description>
     <generator>Hugo -- gohugo.io</generator>
     <language>en-us</language>
-    <lastBuildDate>Thu, 25 Aug 2022 17:16:39 +0800</lastBuildDate><atom:link href="https://leoj.xyz/categories/category/index.xml" rel="self" type="application/rss+xml" />
+    <lastBuildDate>Fri, 26 Aug 2022 16:50:25 +0800</lastBuildDate><atom:link href="https://leoj.xyz/categories/category/index.xml" rel="self" type="application/rss+xml" />
+    <item>
+      <title>Genetics tools</title>
+      <link>https://leoj.xyz/resource/geneticstools/</link>
+      <pubDate>Fri, 26 Aug 2022 16:50:25 +0800</pubDate>
+
+      <guid>https://leoj.xyz/resource/geneticstools/</guid>
+      <description>Mus musculus 129S1 C57BL/6J BALB/cJ
+Mouse genetics tools in brain vasculature system field NG2DsRedBAC: NG2DsRedBAC transgenic mice is suitable for pericyte and Artery SMC labeling in central nervous system. NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS.</description>
+    </item>
+
     <item>
       <title>Biochemistry tools</title>
       <link>https://leoj.xyz/resource/biochemistrytools/</link>
       <pubDate>Thu, 25 Aug 2022 17:16:39 +0800</pubDate>
 
       <guid>https://leoj.xyz/resource/biochemistrytools/</guid>
-      <description>Vendor companies Abcam ThermoFisher R&amp;amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW
-David M. Sabatini lab, MIT
-Xiaodong Wang lab, UTSW, NIBS
-Tool development Alice Ting lab, Stanford </description>
+      <description>Vendor companies Abcam ThermoFisher R&amp;amp;D Systems BioRad Ref Labs Signaling pathway and basic science Zhijian-James Chen lab, UTSW David M. Sabatini lab, MIT Xiaodong Wang lab, UTSW, NIBS Feng Shao lab, NIBS Tool development Alice Ting lab, Stanford Ref. Protocols Bio-protocol Protocol Exchange </description>
     </item>
 
     <item>
@@ -32,11 +39,8 @@ BFPs: TagBFP2, TagBFP
 Reference FPbase: FPbase is a moderated, user-editable fluorescent protein database designed by microscopists. Tissue/Cell type specific AAVs Brain organ specific: BR1
 Liver specific:
 Brain endothelial cell specific: AAV-BI30
-Tissue/Cell type specific promoter Pan-neuron: hSyn Excitatory neuron: CaMKIIa Inhibitory neuron: pGAD1. mDlx
-Constitutive expression: EF1a, CAG
-Drug induced: Tet-on system
-activity depedent: c-Fos promoter
-Genetically encoded indicators Calcium: GCaMP series (GCaMP8, GCaMP7, GCaMP6)</description>
+Tissue/Cell type specific promoter Pan-neuron: hSyn
+Kügler, S., E. Kilic, and M. Bähr. &amp;ldquo;Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area.</description>
     </item>
 
     <item>