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I just got bit by redefining ref as a string:
ref
julia> line = "1\t2\t3\t4\t5\t6" "1\t2\t3\t4\t5\t6" julia> (name, pos, ref, depth, bases, quals) = split(line) 6-element String Array: "1" "2" "3" "4" "5" "6" julia> name[1] type error: apply: expected Function, got ASCIIString