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 Alfie is an alignment-free, kingdom level taxonomic classifier for DNA barcode data. Alfie classifies sequences using a neural network which takes k-mer frequencies (default k = 4) as inputs and makes kingdom level classification predictions. At present, the program contains trained models for classification of cytochrome c oxidase I (COI) barcode sequences to the taxonomic level: kingdom. The program is effective at classifying sequences >200 base pairs in length, and no alignment information is needed.