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Direct shotgun metagenomics captures species abundance of honey samples

This repository contains all files and code to reproduce the results in the publication [add link].

When running the code from scripts, there is no need to change working directories - all paths are relative to the R project file located in this repository.

Overview of folders and description

1. Mocks

This folder contains: 1) A file with the names of the FASTQ files of all mocks used, including the kraken2 evaluation mocks, and links to download them; 2) The true abundance of each organism; 3) The detected abundance using each kraken2, specifically for its threshold evaluation.

2. Figures

It contains a separate folder for each figure. Within the folders, subfolders contain the necessary data as well as the script to produce the figures. The figures will be saved within their folder when the script is run.

3. Output data

Contains the raw figures that were used in the publication. They are deposited here for direct comparison to any changes that may occur when adjusting the scripts.

3. Useful scripts

It contains bash scripts used to generate mocks and evaluate kraken2, mostly for archive purposes and distribution. All custom functions have been combined into the honeyDSM-seq-analysis R package that can be found here:

Summary of packages and session info

current session info
- Session info -----------------------------------------------------------------------------------------------------------------------------------
 setting  value                       
 version  R version 3.6.3 (2020-02-29)
 os       Windows 10 x64              
 system   x86_64, mingw32             
 ui       RStudio                     
 language (EN)                        
 collate  English_United Kingdom.1252 
 ctype    English_United Kingdom.1252 
 tz       Europe/Istanbul             
 date     2020-10-06                  

- Packages ---------------------------------------------------------------------------------------------------------------------------------------
 package              * version  date       lib source        
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 ape                    5.3      2019-03-17 [1] CRAN (R 3.6.3)
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[1] C:/Users/user/Documents/R/win-library/3.6
[2] C:/Program Files/R/R-3.6.3/library