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# Schedule for Single-cell RNA-seq workshop

## Pre-reading
* [Introduction to scRNA-seq](../lessons/01_intro_to_scRNA-seq.md)
* [Raw data to count matrix](../lessons/02_SC_generation_of_count_matrix.md)
* Download this project!!
* Install these libraries

## Day 1
| Time | Topic | Instructor |
|:-----------:|:----------:|:--------:|
| 09:30 - 09:50 | [Workshop introduction](../slides/Intro_to_workshop.pdf) | Radhika
| 09:50 - 10:05 | [R refresher Q & A](../lessons/R_refresher.md) | Mary |
| 10:05 - 10:35 | scRNA-seq pre-reading discussion | Meeta/Mary |
| 10:35 - 11:20 | [Quality control set-up](../lessons/03_SC_quality_control-setup.md) | Jihe |
| 11:20 - 12:00 | [Single-cell RNA-seq design and methods](../slides/Single_Cell_2_27_20.pdf) | [Dr. Sarah Boswell](https://scholar.harvard.edu/saboswell) |

### Self Learning #1
* [Quality control](../lessons/04_SC_quality_control.md)
* [Theory of Normalization and PCA](../lessons/05_normalization_and_PCA.md)
* (after split) Normalization

## Day 2

| Time | Topic | Instructor |
|:-----------:|:----------:|:--------:|
| 1h | Self-learning lessons discussion | Meeta/Mary |
| 1h | (after split) Integration | Mary |

### Self Learning #2
* [Clustering](../lessons/07_SC_clustering_cells_SCT.md)
* [Clustering quality control](../lessons/08_SC_clustering_quality_control.md)
* [Marker identification](../lessons/09_merged_SC_marker_identification.md)

## Day 3

| Time | Topic | Instructor |
|:-----------:|:----------:|:--------:|
| 1h | Self-learning lessons discussion | Meeta/Mary |
| 1h | Discussion, Final Q & A | All |
| 30m| [Wrap up](../slides/Workshop_wrapup.pdf) | Radhika |

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