diff --git a/samples/bluetooth/bap_broadcast_source/overlay-interleaved.conf b/samples/bluetooth/bap_broadcast_source/overlay-interleaved.conf
new file mode 100644
index 000000000000000..837004a344dd814
--- /dev/null
+++ b/samples/bluetooth/bap_broadcast_source/overlay-interleaved.conf
@@ -0,0 +1 @@
+CONFIG_BT_ISO_PACKING_INTERLEAVED=y
diff --git a/samples/bluetooth/bap_broadcast_source/overlay-sequential.conf b/samples/bluetooth/bap_broadcast_source/overlay-sequential.conf
new file mode 100644
index 000000000000000..5a59893eaf7795c
--- /dev/null
+++ b/samples/bluetooth/bap_broadcast_source/overlay-sequential.conf
@@ -0,0 +1 @@
+CONFIG_BT_ISO_PACKING_INTERLEAVED=n
diff --git a/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_interleaved.sh b/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_interleaved.sh
new file mode 100755
index 000000000000000..0ede3944b7a373e
--- /dev/null
+++ b/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_interleaved.sh
@@ -0,0 +1,29 @@
+#!/usr/bin/env bash
+# Copyright 2023 Nordic Semiconductor ASA
+# SPDX-License-Identifier: Apache-2.0
+
+# Simple selfchecking test for the broadcast audio sink/source samples,
+# It relies on the bs_tests hooks to register a test timer callback, which after a deadline
+# will check how many audio packets the broadcast audio sink has received, and if over a threshold
+# it considers the test passed
+
+simulation_id="samples_bluetooth_bap_broadcast_source_interleaved_test"
+sample_long_name="samples_bluetooth_bap_broadcast_source"
+verbosity_level=2
+EXECUTE_TIMEOUT=200
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+cd ${BSIM_OUT_PATH}/bin
+
+Execute ./bs_${BOARD_TS}_${sample_long_name}_prj_conf_overlay-interleaved_conf \
+  -v=${verbosity_level} -s=${simulation_id} -d=0 -RealEncryption=1
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_samples_bap_broadcast_sink_prj_conf \
+  -v=${verbosity_level} -s=${simulation_id} -d=1 -RealEncryption=1 \
+  -testid=bap_broadcast_sink
+
+Execute ./bs_2G4_phy_v1 -v=${verbosity_level} -s=${simulation_id} \
+  -D=2 -sim_length=120e6 $@
+
+wait_for_background_jobs #Wait for all programs in background and return != 0 if any fails
diff --git a/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio.sh b/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_sequential.sh
similarity index 90%
rename from tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio.sh
rename to tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_sequential.sh
index bb7230ca53efc06..91b0889817fa905 100755
--- a/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio.sh
+++ b/tests/bsim/bluetooth/audio_samples/bap_broadcast_sink/tests_scripts/broadcast_audio_sequential.sh
@@ -7,16 +7,15 @@
 # will check how many audio packets the broadcast audio sink has received, and if over a threshold
 # it considers the test passed
 
-simulation_id="broadcast_audio_samples_test"
+simulation_id="samples_bluetooth_bap_broadcast_source_sequential_test"
 verbosity_level=2
+EXECUTE_TIMEOUT=200
 
 source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
 
-EXECUTE_TIMEOUT=200
-
 cd ${BSIM_OUT_PATH}/bin
 
-Execute ./bs_${BOARD_TS}_samples_bluetooth_bap_broadcast_source_prj_conf \
+Execute ./bs_${BOARD_TS}_samples_bluetooth_bap_broadcast_source_prj_conf_overlay-sequential_conf \
   -v=${verbosity_level} -s=${simulation_id} -d=0 -RealEncryption=1
 
 Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_samples_bap_broadcast_sink_prj_conf \
diff --git a/tests/bsim/bluetooth/audio_samples/compile.sh b/tests/bsim/bluetooth/audio_samples/compile.sh
index d2d945df52a451e..b37c56f1867eb83 100755
--- a/tests/bsim/bluetooth/audio_samples/compile.sh
+++ b/tests/bsim/bluetooth/audio_samples/compile.sh
@@ -13,7 +13,10 @@ source ${ZEPHYR_BASE}/tests/bsim/compile.source
 
 if [ "${BOARD_TS}" == "nrf5340bsim_nrf5340_cpuapp" ]; then
   app=samples/bluetooth/bap_unicast_server sysbuild=1 compile
-  app=samples/bluetooth/bap_broadcast_source sysbuild=1 compile
+  app=samples/bluetooth/bap_broadcast_source conf_overlay=overlay-sequential.conf \
+    sysbuild=1 compile
+  app=samples/bluetooth/bap_broadcast_source conf_overlay=overlay-interleaved.conf \
+    sysbuild=1 compile
   app=tests/bsim/bluetooth/audio_samples/bap_unicast_client \
     sample=${ZEPHYR_BASE}/samples/bluetooth/bap_unicast_client \
     conf_file=${sample}/prj.conf \
@@ -41,8 +44,12 @@ if [ "${BOARD_TS}" == "nrf5340bsim_nrf5340_cpuapp" ]; then
 else
   app=samples/bluetooth/bap_unicast_server conf_overlay=overlay-bt_ll_sw_split.conf \
     exe_name=bs_${BOARD_TS}_${app}_prj_conf sysbuild=1 compile
-  app=samples/bluetooth/bap_broadcast_source conf_overlay=overlay-bt_ll_sw_split.conf \
-    exe_name=bs_${BOARD_TS}_${app}_prj_conf sysbuild=1 compile
+  app=samples/bluetooth/bap_broadcast_source \
+    conf_overlay='overlay-bt_ll_sw_split.conf;overlay-sequential.conf' \
+    exe_name=bs_${BOARD_TS}_${app}_prj_conf_overlay-sequential_conf sysbuild=1 compile
+  app=samples/bluetooth/bap_broadcast_source \
+    conf_overlay='overlay-bt_ll_sw_split.conf;overlay-interleaved.conf' \
+    exe_name=bs_${BOARD_TS}_${app}_prj_conf_overlay-interleaved_conf sysbuild=1 compile
   app=tests/bsim/bluetooth/audio_samples/bap_unicast_client \
     sample=${ZEPHYR_BASE}/samples/bluetooth/bap_unicast_client \
     conf_file=${sample}/prj.conf \