diff --git a/README.md b/README.md index 8932f4e..998a0e7 100644 --- a/README.md +++ b/README.md @@ -1,7 +1,12 @@ # STAARpipeline-Tutorial This is a tutorial for (1) automatically functionally annotating the variants of whole-genome/whole-exome sequencing (WGS/WES) studies and integrating the functional annotations with the genotype data using **FAVORannotator** and (2) performing association analysis of WGS/WES studies, summarizing and visualization results using **STAARpipeline** and **STAARpipelineSummary**. The software prerequisites, dependencies and installation can be found in **STAARpipeline** and **STAARpipelineSummary** packages. -**FAVORannotator**, **STAARpipeline** and **STAARpipelineSummary** are implemented as a collection of apps. Please see the apps **favorannotator**, **staarpipeline**, **staarpipelinesummary_varset** and **staarpipelinesummary_indvar** that run on the UK Biobank Research Analysis Platform for more details. +**FAVORannotator**, **STAARpipeline** and **STAARpipelineSummary** are implemented as a collection of apps. Please see the apps +**favorannotator (Seven Bridges)**, **favorannotator (DNAnexus)** +**staarpipeline (Seven Bridges)**, **staarpipeline (DNAnexus)** +**staarpipelinesummary_varset (Seven Bridges)**, **staarpipelinesummary_varset (DNAnexus)** +**staarpipelinesummary_indvar (Seven Bridges)**, **staarpipelinesummary_indvar (DNAnexus)** +that run on the NIH/NHLBI BioData Catalyst (BDC) ecosystem and the UK Biobank Research Analysis Platform (RAP) for more details (**user manual and tutorial**). ## Pre-step of association analysis using STAARpipeline ### Generate Genomic Data Structure (GDS) file R/Bioconductor package **SeqArray** provides functions to convert the genotype data (in VCF/BCF/PLINK BED/SNPRelate format) to SeqArray GDS format. For more details on usage, please see the R/Bioconductor package **SeqArray** [manual]. A wrapper for the `seqVCF2GDS`/`seqBCF2GDS` function in the SeqArray package can be found **here** (**Credit: Michael R. Brown and Jennifer A. Brody**).