Necessary columns:
- cell_line = name of the cell line used in the experiment
- treatment = name of the treatment used in the experiment
- concentration = concentration value (not log-transformed) of the treatment (all concentrations must be positive numbers)
- cell_count = treated cell count (end of assay) - measure of cell number in the treated well at the end of the assay
- cell_count__ctrl - control cell count (end of assay) - measure of cell number in the control well (e.g. untreated or DMSO-treated) from the same plate at the end of the assay
- cell_count__time0 - initial cell count (time of treatment) - measure of cell number at the beginning of the assay. (measure of cells in an untreated well grown in parallel until the time of treatment)
You may use a surrogate of cell number (such as CellTiter-Glo® staining) for the 'cell counts'.
You may include additional columns to be used for grouping the dose-response experiments.