diff --git a/README.md b/README.md
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   <img src="https://contrib.rocks/image?repo=shangshanzhizhe/Work_flow_of_population_genetics" />
 </a>
 
->[Mou Yin](https://github.com/yinm2018): 看看我写的基因组学研究流程！\
-[Hermiolee1](https://github.com/Hermiolee1): 我占坑的，怎么了嘛
 
 ### 下一步
 
diff --git a/Work_flows/transcriptome.md b/Work_flows/transcriptome.md
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+# 比较转录组学分析
+
+## 序列比对
+
+### 原始数据质量控制
+
+使用fastq
+
+###  建立基因组索引
+
+```sh
+hisat2-build -c reference.fasta creference
+```
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