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Hi evryone!
I followed the instructions as the example and everything is ik, but i´m trying to do the same with my data (I have 16s sequencing data processed with qiime2 from different locations). But i don´t know how to generate D
as i read, D might be done with distance_mat function, but that function asks me for 3 inputs:
edges <- a matrix of dimension N*2 corresponding to the edge set of phylogenetic tree
n_taxa <- A scalar corresponding to number of taxa in the dataset
tree_height <- A scalar corresponding to the height of the phylogenetic tree.
And i dont know how to get those inputs.
I only have:
Representative sequences
otu table
metadata file
phylogenetic tree (made with iqtree within qiime2)
Does anyone if i might do the same as the example with my data?
thanks
The text was updated successfully, but these errors were encountered:
Hi evryone!
I followed the instructions as the example and everything is ik, but i´m trying to do the same with my data (I have 16s sequencing data processed with qiime2 from different locations). But i don´t know how to generate D
mbImpute(condition = location, otu_tab = otu_table, metadata = manifest, D = ?)
as i read, D might be done with
distance_mat
function, but that function asks me for 3 inputs:edges <- a matrix of dimension N*2 corresponding to the edge set of phylogenetic tree
n_taxa <- A scalar corresponding to number of taxa in the dataset
tree_height <- A scalar corresponding to the height of the phylogenetic tree.
And i dont know how to get those inputs.
I only have:
Representative sequences
otu table
metadata file
phylogenetic tree (made with iqtree within qiime2)
Does anyone if i might do the same as the example with my data?
thanks
The text was updated successfully, but these errors were encountered: