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short read data #23

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rmormando opened this issue Dec 11, 2023 · 1 comment
Open

short read data #23

rmormando opened this issue Dec 11, 2023 · 1 comment
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@rmormando
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I have Illumina short-read sequences I want to use as an input will the tool take that into account when creating a database?

The samples are labeled asSample1_R1.fasta Sample1_R2.fasta would I put that all into the input list file so it reads like:

Sample1_R1
Sample1_R2
Sample2_R1
Sample2_R2
....
Sample20_R1
Sample20_R2

Would the tool recognize short read inputs? Or should I merge the two files so it only takes one input for that particular sample?

@agudys agudys self-assigned this Apr 12, 2024
@agudys
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agudys commented Apr 12, 2024

Hello!

Sorry for the delay in answer - I have somehow missed your issue! Every entry in the file list would be considered as a separate sample. So if you have two files for paired-end reads, you would need to concatenate them prior to the analysis, exactly as you said.

Best,
Adam

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