MerCat2: python code for versatile k-mer counter for database independent property analysis (DIPA) for omic analysis

Installing MerCat2

Option 1: Bioconda Installer

Install mamba using conda

conda activate base
conda install mamba

• NOTE: Make sure you install mamba in your base conda environment
• We have found that mamba is faster than conda for installing packages and creating environments. Using conda might fail to resolve dependencies.

Install MerCat2

• Available via Bioconda:

mamba create -n mercat2 -c conda-forge -c bioconda mercat2
conda activate mercat2

Option 2: Source Installer

• Clone mercat2 from github
• Run install_mercat2.sh to install all required dependencies
• This script creates a conda environment for you

git clone https://github.com/raw-lab/mercat2.git
cd mercat2
bash install_mercat2.sh
conda activate mercat2

Dependencies

MerCat2 runs on python version 3.9 and higher.

external dependencies

MerCat2 can run without external dependencies based on the options used.

Required dependencies:

• When a raw read .fastq file is given

  • Fastqc
  • Fastp

• For bacteria/archaea rich samples (-prod option)

  • Prodigal

• For eukaryote rich samples or general applications (-fgs option)

  • FragGeneScanRs

These are available through BioConda, except FragGeneScanRS, which is included in the MerCat2 distribution.

conda install -c bioconda fastqc fastp prodigal

Usage

usage: mercat2.py [-h] [-i I [I ...]] [-f F] -k K [-n N] [-c C] [-prod] [-fgs] [-s S] [-o O] [-replace] [-lowmem LOWMEM] [-skipclean] [-toupper] [-pca] [--version]

options:
  -h, --help      show this help message and exit
  -i I            path to input file(s)
  -f F            path to folder containing input files
  -k K            kmer length
  -n N            no of cores [auto detect]
  -c C            minimum kmer count [10]
  -prod           run Prodigal on fasta files
  -fgs            run FragGeneScanRS on fasta files
  -s S            Split into x MB files. [100]
  -o O            Output folder, default = 'mercat_results' in current directory
  -replace        Replace existing output directory [False]
  -lowmem LOWMEM  Flag to use incremental PCA when low memory is available. [auto]
  -skipclean      skip trimming of fastq files
  -toupper        convert all input sequences to uppercase
  -pca            create interactive PCA plot of the samples (minimum of 4 fasta files required)
  --version, -v   show the version number and exit

Mercat assumes the input file format based on the extension provided

• raw fastq file: ['.fastq', '.fq']

• nucleotide fasta: ['.fa', '.fna', '.ffn', '.fasta']

• amino acid fasta: ['.faa']

• It also accepts gzipped versions of these filetypes with the added '.gz' suffix

Usage examples

Run mercat2 on a protein file (protein fasta - '.faa')

mercat2.py -i file-name.faa -k 3 -c 10

Run mercat2 on a nucleotide file (nucleotide fasta - '.fa', '.fna', '.ffn', '.fasta')

mercat2.py -i file-name.fna -k 3 -n 8 -c 10

Run mercat2 on a nucleotide file raw data (nucleotide fastq - '.fastq')

mercat2.py -i file-name.fastq -k 3 -n 8 -c 10

Run on many samples within a folder

mercat2.py -f /path/to/input-folder -k 3 -n 8 -c 10

Run on sample with prodigal/FragGeneScanRS option (raw reads or nucleotide contigs - '.fa', '.fna', '.ffn', '.fasta', '.fastq')

mercat2.py -i /path/to/input-file -k 3 -n 8 -c 10 -prod

mercat2.py -i /path/to/input-file -k 3 -n 8 -c 10 -fgs

• the prodigal and FragGeneScanRS options run the k-mer counter on both contigs and produced amino acids

Outputs

• Results are stored in the output folder (default 'mercat_results' of the current working directory)
  • the 'report' folder contains an html report with interactive plotly figures
    • If at least 4 samples are provided a PCA plot will be included in the html report
  • the 'tsv' folder contains counts tables in tab separated format
    • if protein files are given, or the -prod option, a .tsv file is created for each sample containing k-mer count, pI, Molecular Weight, and Hydrophobicity metrics
    • if nucleotide files are given a .tsv file is created for each sample containing k-mer count and GC content
  • if .fastq raw reads files are used, a 'clean' folder is created with the clean fasta file.
  • if the -prod option is used, a 'prodigal' folder is created with the amino acid .faa and .gff files
  • if the -fgs option is used, a 'fgs' folder is created with the amino acid .faa file

Diversity estimation

Alpha and Beta diversity metrics provided by MerCat2 are experimental. We are currently working on the robustness of these measures.

Alpha diversity metrics provided:

• shannon
• simpson
• simpson_e
• goods_coverage
• fisher_alpha
• dominance
• chao1
• chao1_ci
• ace

Beta diversity metrics provided:

• euclidean
• cityblock
• braycurtis
• canberra
• chebyshev
• correlation
• cosine
• dice
• hamming
• jaccard
• mahalanobis
• manhattan (same as City Block in this case)
• matching
• minkowski
• rogerstanimoto
• russellrao
• seuclidean
• sokalmichener
• sokalsneath
• sqeuclidean
• yule

Notes on memory usage and speed

MerCat2 uses a substantial amount of memory when the k-mer is high.
Running MerCat2 on a personal computer using a k-mer length of ~4 should be OK. Total memory usage can be reduced using the Chunker feature (-s option), but keep in mind that in testing when the chunk size is too small (1MB) some of the least significant k-mers will get lost. This does not seem to affect the overall results, but it is something to keep in mind. Using the chunker and reducing the number of CPUs available (-n option) can help reduce memory requirements.

The speed of MerCat2 can be increased when more memory or computer nodes are available on a cluster and using a chunk size of about 100Mb.

Copyright

This is copyrighted by University of North Carolina at Charlotte, Jose L. Figueroa III, Andrew Redinbo, and Richard Allen White III. All rights reserved. DeGenPrime is a bioinformatic tool that can be distributed freely for academic use only. Please contact us for commerical use. The software is provided “as is” and the copyright owners or contributors are not liable for any direct, indirect, incidental, special, or consequential damages including but not limited to, procurement of goods or services, loss of use, data or profits arising in any way out of the use of this software.

Citing Mercat

If you are publishing results obtained using MerCat2, please cite:
Figueroa JL*, Redinbo A*, Panyala A, Colby S, Friesen M, Tiemann L, White III RA. 2024.
MerCat2: a versatile k-mer counter and diversity estimator for database-independent property analysis obtained from omics data
Bioinformatics Advances, vbae061 Bioinformatics Advances
*Co-first authors

BioRxiv pre-print
Figueroa JL, Panyala A, Colby S, Friesen M, Tiemann L, White III RA. 2022.
MerCat2: a versatile k-mer counter and diversity estimator for database-independent property analysis obtained from omics data.
bioRxiv

CONTACT

Please send all queries to Jose Luis Figueroa III
Dr. Richard Allen White III
Andrew Redinbo

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