diff --git a/chrome/app/chromeos_strings.grdp b/chrome/app/chromeos_strings.grdp
index 5405c012894c2f..a7b21fd3971fa0 100644
--- a/chrome/app/chromeos_strings.grdp
+++ b/chrome/app/chromeos_strings.grdp
@@ -120,12 +120,6 @@ Press any key to continue exploring.
   </message>
 
   <!-- File Manager -->
-  <message name="IDS_FLAGS_FILE_MANAGER_SHOW_CHECKBOXES_NAME" desc="Name of the about:flag option to show the selecting checkboxes to the Files app.">
-    Show selecting checkboxes
-  </message>
-  <message name="IDS_FLAGS_FILE_MANAGER_SHOW_CHECKBOXES_DESCRIPTION" desc="Description of the about:flag option to show the selecting checkboxes to the Files app.">
-    Show selecting checkboxes in the Files.app.
-  </message>
   <message name="IDS_FLAGS_FILE_MANAGER_ENABLE_NEW_AUDIO_PLAYER_NAME" desc="Name of the about:flag option to enable the new audio player app.">
     Enable the new audio player
   </message>
diff --git a/chrome/browser/about_flags.cc b/chrome/browser/about_flags.cc
index a89f4ef66d0bb6..06c1b1ec147e69 100644
--- a/chrome/browser/about_flags.cc
+++ b/chrome/browser/about_flags.cc
@@ -1125,13 +1125,6 @@ const Experiment kExperiments[] = {
     kOsCrOSOwnerOnly,
     SINGLE_VALUE_TYPE(chromeos::switches::kDisableBootAnimation),
   },
-  {
-    "file-manager-show-checkboxes",
-    IDS_FLAGS_FILE_MANAGER_SHOW_CHECKBOXES_NAME,
-    IDS_FLAGS_FILE_MANAGER_SHOW_CHECKBOXES_DESCRIPTION,
-    kOsCrOS,
-    SINGLE_VALUE_TYPE(chromeos::switches::kFileManagerShowCheckboxes)
-  },
   {
     "enable-new-audio-player",
     IDS_FLAGS_FILE_MANAGER_ENABLE_NEW_AUDIO_PLAYER_NAME,
diff --git a/chromeos/chromeos_switches.cc b/chromeos/chromeos_switches.cc
index 028b30adffdf02..d546967b90d494 100644
--- a/chromeos/chromeos_switches.cc
+++ b/chromeos/chromeos_switches.cc
@@ -134,9 +134,6 @@ const char kEnterpriseEnrollmentModulusLimit[] =
 const char kEnterpriseEnrollmentSkipRobotAuth[] =
     "enterprise-enrollment-skip-robot-auth";
 
-// Shows the selecting checkboxes in the Files.app.
-const char kFileManagerShowCheckboxes[]     = "file-manager-show-checkboxes";
-
 // Enables the new audio player in the Files.app.
 const char kFileManagerEnableNewAudioPlayer[] =
     "file-manager-enable-new-audio-player";
diff --git a/chromeos/chromeos_switches.h b/chromeos/chromeos_switches.h
index 722fde65a6c17e..44e4b9447a12b3 100644
--- a/chromeos/chromeos_switches.h
+++ b/chromeos/chromeos_switches.h
@@ -54,7 +54,6 @@ CHROMEOS_EXPORT extern const char kEnabledStubNetworkTypes[];
 CHROMEOS_EXPORT extern const char kEnterpriseEnrollmentInitialModulus[];
 CHROMEOS_EXPORT extern const char kEnterpriseEnrollmentModulusLimit[];
 CHROMEOS_EXPORT extern const char kEnterpriseEnrollmentSkipRobotAuth[];
-CHROMEOS_EXPORT extern const char kFileManagerShowCheckboxes[];
 CHROMEOS_EXPORT extern const char kFileManagerEnableNewAudioPlayer[];
 CHROMEOS_EXPORT extern const char kFirstExecAfterBoot[];
 CHROMEOS_EXPORT extern const char kForceLoginManagerInTests[];