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README.md

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@@ -236,7 +236,7 @@ https://www.dropbox.com/s/vrqc7bxxwsyd83g/DeduceRelationship_Tutorial.html?dl=0
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# ELeFHAnt Reference datasets as plugins
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Download pre-processed reference datasets for Celltype Annotation, Label Harmonization or DeduceRelationship here: https://www.dropbox.com/sh/6hd2skriqqlokwp/AAAVol-_qPlCdA4DpERWjkeJa?dl=0
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# Datasets used to showcase ELeFHAnt functionalities
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To demonstrate all the functions of ELeFHAnt we utilize three datasets of early gut development as shown below, as well as an integrated dataset of all three. "GSE158702" refers to a subset of terminal ileum (TI) data from an atlas for human fetal intestinal development called "STAR-FINDer" (https://www.sciencedirect.com/science/article/pii/S009286742031686X). "E-MTAB-8901" refers to a subset of duojejunum cell data from the Gut Cell Atlas, which also examines intestinal development from 6-10 weeks post-conception (https://www.sciencedirect.com/science/article/pii/S1534580720308868). Lastly, "E-MTAB-10187" refers a subset of fetal intestinal data from a multi-endodermal organ atlas (https://www.sciencedirect.com/science/article/pii/S0092867421005316).
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![Graph](Examples/gut_datasets.png)
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# Label Harmonization Example
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To demonstrate LabelHarmonization we used three datasets: 1) Gut Cell Atlas (https://www.sciencedirect.com/science/article/pii/S1534580720308868) 2) Fetal intestinal data (https://www.sciencedirect.com/science/article/pii/S0092867421005316) from Dr. Spence's Lab 3) Fetal intestine data from STAR-FINDer (https://www.sciencedirect.com/science/article/pii/S009286742031686X). Data shown below is based on subsetting 200 cells per celltype in each dataset to harmonize atlas consisting of ~125k cells.
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### Harmonized Atlas ~125k cells
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![Graph](Examples/HarmonizationExample_ELeFHAnt.png)
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To demonstrate LabelHarmonization we used three datasets: 1) E-MTAB-8901 (~21k cells) 2) E-MTAB-10187 (~77k cells) 3) GSE158702 (~20k cells). We integrated three datasets using Seurat's CCA based integration and then ran Label Harmonization (downsample = TRUE, downsample_to = 500) on the integrated object (~112k cells). Left panel shows all ~120 cell types whereas right panel shows 33 granular celltypes assigned after harmonization.
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### Harmonized Atlas ~112k cells
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![Graph](Examples/LabelHarmonization.png)
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We can clearly see that after integration showing Celltypes from each dataset on UMAP is very difficult to interpret. Whereas using LabelHarmonization from ELeFHAnt each cluster after integration has a clear celltype assignment, making interpretation much clearer.
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