diff --git a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
index 8e8f7e34ee..ce302a6394 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
@@ -335,7 +335,6 @@ def check_headers(**kwargs):
         )
         ec2.describe_instances()
 
-        request_id = "fdcdcab1-ae5c-489e-9c33-4637c5dda355"
 
         self.assertNotIn(MockTextMapPropagator.TRACE_ID_KEY, headers)
         self.assertNotIn(MockTextMapPropagator.SPAN_ID_KEY, headers)