diff --git a/instrumentation/opentelemetry-instrumentation-pymysql/pyproject.toml b/instrumentation/opentelemetry-instrumentation-pymysql/pyproject.toml
index 4018d29601..9e6028bf64 100644
--- a/instrumentation/opentelemetry-instrumentation-pymysql/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-pymysql/pyproject.toml
@@ -33,10 +33,6 @@ dependencies = [
 instruments = [
   "PyMySQL < 2",
 ]
-test = [
-  "opentelemetry-instrumentation-pymysql[instruments]",
-  "opentelemetry-test-utils == 0.45b0.dev",
-]
 
 [project.entry-points.opentelemetry_instrumentor]
 pymysql = "opentelemetry.instrumentation.pymysql:PyMySQLInstrumentor"
diff --git a/instrumentation/opentelemetry-instrumentation-pymysql/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-pymysql/test-requirements.txt
new file mode 100644
index 0000000000..1ddfa51e0b
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-pymysql/test-requirements.txt
@@ -0,0 +1,19 @@
+asgiref==3.7.2
+attrs==23.2.0
+Deprecated==1.2.14
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+packaging==23.2
+pluggy==1.4.0
+py==1.11.0
+py-cpuinfo==9.0.0
+PyMySQL==1.1.0
+pytest==7.1.3
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.9.0
+wrapt==1.16.0
+zipp==3.17.0
+-e ../../../opentelemetry-instrumentation
+-e ../../../instrumentation/opentelemetry-instrumentation-dbapi
+-e ../../../instrumentation/opentelemetry-instrumentation-pymysql
diff --git a/tox.ini b/tox.ini
index 74f785974d..ca711160ad 100644
--- a/tox.ini
+++ b/tox.ini
@@ -427,7 +427,7 @@ commands_pre =
 
   psycopg2: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi {toxinidir}/instrumentation/opentelemetry-instrumentation-psycopg2[test]
 
-  pymysql: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi {toxinidir}/instrumentation/opentelemetry-instrumentation-pymysql[test]
+  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pymysql
 
   pyramid: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-pyramid[test]
 
@@ -564,7 +564,7 @@ commands_pre =
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-requests[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-urllib[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-urllib3[test]
-  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pymysql[test]
+  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pymysql
   # prerequisite: follow the instructions here https://github.com/PyMySQL/mysqlclient#install
   # for your OS to install the required dependencies
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-mysqlclient[test]